BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,423	C→T	6.9%	Q169Q (CAG→CAA)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,423	0	C	T	6.9%	370.4 / 12.1	115	Q169Q (CAG→CAA)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base C (46/61); new base T (2/6); total (48/67)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 121 total aligned reads displayed.

CCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTT  >  minE/2677356‑2677492

ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                                     <  1:3055109/70‑1 (MQ=255)

ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                                     <  1:23658/70‑1 (MQ=255)

 cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                                   <  1:1888385/71‑1 (MQ=255)

  aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                                  <  1:2273773/71‑1 (MQ=255)

  aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                                  >  1:213696/1‑71 (MQ=255)

  aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                                  <  1:3037099/71‑1 (MQ=255)

   gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                                  >  1:686605/1‑70 (MQ=255)

   atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt                                                                  <  1:2748717/68‑1 (MQ=255)

        aacAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                                                            <  1:867482/69‑1 (MQ=255)

        aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                                                                <  1:3594158/67‑1 (MQ=255)

         tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                                                           >  1:3925326/1‑71 (MQ=255)

           aGGTCGCGTTGATGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                                                              <  1:1152073/66‑1 (MQ=255)

           aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                                                  <  1:2743089/62‑1 (MQ=255)

           aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                          >  1:2628654/1‑70 (MQ=255)

           aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                          >  1:3342403/1‑70 (MQ=255)

           aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                          >  1:1449557/1‑70 (MQ=255)

             atCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                                                 <  1:1673469/60‑2 (MQ=255)

              tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatc                                                      <  1:2789595/71‑1 (MQ=255)

               cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                       >  1:3066812/1‑69 (MQ=255)

               cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                       >  1:1064608/1‑69 (MQ=255)

               cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                       >  1:2633924/1‑69 (MQ=255)

               cgcgCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                                     >  1:3869491/1‑70 (MQ=255)

                gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                                     <  1:649728/70‑2 (MQ=255)

                gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                                                    >  1:2643547/1‑55 (MQ=255)

                gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                                     <  1:643106/70‑1 (MQ=255)

                 cgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                                   <  1:213280/71‑1 (MQ=255)

                   ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                 >  1:579528/1‑71 (MQ=255)

                    tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                >  1:3965443/1‑71 (MQ=255)

                     gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                >  1:2668514/1‑70 (MQ=255)

                     gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                <  1:2432739/70‑1 (MQ=255)

                       cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                          >  1:2060623/1‑58 (MQ=255)

                        gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                 >  1:515905/1‑66 (MQ=255)

                        gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATg                                               <  1:2833312/68‑1 (MQ=255)

                        gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                             <  1:2928519/70‑1 (MQ=255)

                        gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                             <  1:743027/70‑1 (MQ=255)

                         aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                             <  1:1756823/69‑1 (MQ=255)

                          atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                                                   >  1:1036151/1‑46 (MQ=255)

                          atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                          <  1:226263/71‑1 (MQ=255)

                          atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                          <  1:1742926/71‑1 (MQ=255)

                          atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                          >  1:949823/1‑71 (MQ=255)

                          atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                          <  1:2747591/71‑1 (MQ=255)

                          atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                          <  1:972013/71‑1 (MQ=255)

                           tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg                                         <  1:3971231/71‑1 (MQ=255)

                             tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa                                              >  1:1157143/1‑64 (MQ=255)

                             tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                        >  1:4068641/1‑70 (MQ=255)

                             tGTTTTTGAATTAACTCCAGCAGCGACATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                        >  1:253024/1‑70 (MQ=255)

                               tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                                                 <  1:133888/43‑1 (MQ=255)

                               tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                                     >  1:96637/1‑55 (MQ=255)

                               tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                          <  1:492058/66‑1 (MQ=255)

                                ttctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGTAATatc                                    >  1:2935972/4‑71 (MQ=255)

                                 tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                                   <  1:3571142/55‑1 (MQ=255)

                                 tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                                    <  1:3514089/70‑1 (MQ=255)

                                 tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                                    <  1:2776165/70‑1 (MQ=255)

                                  ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                 >  1:2054602/1‑56 (MQ=255)

                                  ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatca                                   <  1:1885155/70‑1 (MQ=255)

                                   tGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                                 >  1:2162209/1‑71 (MQ=255)

                                      aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                              <  1:2642815/71‑1 (MQ=255)

                                      aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                              >  1:3741100/1‑71 (MQ=255)

                                      aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                              >  1:3365182/1‑71 (MQ=255)

                                       ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                                   <  1:305933/49‑1 (MQ=255)

                                       ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGc                             >  1:1876252/1‑71 (MQ=255)

                                          aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                          <  1:1013238/71‑1 (MQ=255)

                                          aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                          <  1:1965513/71‑1 (MQ=255)

                                            tCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                            <  1:2435502/35‑1 (MQ=255)

                                              cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                          <  1:642744/51‑1 (MQ=255)

                                              cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                          <  1:92304/51‑1 (MQ=255)

                                              cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                          <  1:886151/67‑1 (MQ=255)

                                              cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                          <  1:940192/67‑1 (MQ=255)

                                              cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt                       <  1:1429735/70‑1 (MQ=255)

                                               agcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc                     <  1:13675/71‑1 (MQ=255)

                                                gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    >  1:927319/1‑71 (MQ=255)

                                                gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    <  1:1155648/71‑1 (MQ=255)

                                                gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    >  1:591913/1‑71 (MQ=255)

                                                gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    >  1:1354640/1‑71 (MQ=255)

                                                gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    >  1:1790572/1‑71 (MQ=255)

                                                gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    <  1:2711612/71‑1 (MQ=255)

                                                  agcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGa                  <  1:1081708/71‑1 (MQ=255)

                                                   gcgcCATACGTCGGTGCTGCATGATTTAATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg                 >  1:3446834/1‑71 (MQ=255)

                                                    cgcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg                <  1:1393371/71‑1 (MQ=255)

                                                     gcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg                >  1:3236335/1‑70 (MQ=255)

                                                        aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatca                               <  1:1130800/52‑1 (MQ=255)

                                                        aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCATTCAGCGGAAATGCGGAGGCATa            <  1:3300270/71‑1 (MQ=255)

                                                        aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt             >  1:3647826/1‑70 (MQ=255)

                                                        aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATa            <  1:873938/71‑1 (MQ=255)

                                                         tACGTCGGTGCTGCATGATTTCATCATCAGGGATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa           <  1:753398/71‑1 (MQ=255)

                                                         tACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg                 <  1:757004/65‑1 (MQ=255)

                                                          aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaaa                        >  1:794964/1‑57 (MQ=255)

                                                          aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc                     <  1:3208772/60‑1 (MQ=255)

                                                          aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                    <  1:465720/61‑1 (MQ=255)

                                                          aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa              <  1:23824/67‑1 (MQ=255)

                                                           cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                                 >  1:1810413/1‑47 (MQ=255)

                                                           cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                                 >  1:3225989/1‑47 (MQ=255)

                                                           cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGc               >  1:424480/1‑65 (MQ=255)

                                                           cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAg         >  1:1005280/1‑71 (MQ=255)

                                                               ggTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTg      <  1:2016281/70‑1 (MQ=255)

                                                               ggTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTg      <  1:1940919/70‑1 (MQ=255)

                                                               ggTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGc     >  1:3309732/1‑71 (MQ=255)

                                                                 tgctgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCt   <  1:3207245/71‑1 (MQ=255)

                                                                   ctgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaa                         <  1:3026034/47‑1 (MQ=255)

                                                                   ctgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCtt  <  1:2148178/70‑1 (MQ=255)

CCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTT  >  minE/2677356‑2677492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`