BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,429	G→A	5.2%	I167I (ATC→ATT)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,429	0	G	A	5.2%	385.9 / 6.9	114	I167I (ATC→ATT)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base G (46/62); new base A (2/4); total (48/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 117 total aligned reads displayed.

CCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTTGCCAGT  >  minE/2677361‑2677498

cccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAt                                                                     <  1:2768629/71‑1 (MQ=255)

   aacAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                                                                  <  1:867482/69‑1 (MQ=255)

    tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                                                       <  1:2665079/65‑1 (MQ=255)

    tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                                                                 >  1:3925326/1‑71 (MQ=255)

      aGGTCGCGTTGATGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                                                                    <  1:1152073/66‑1 (MQ=255)

      aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                                >  1:2628654/1‑70 (MQ=255)

      aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                                >  1:1449557/1‑70 (MQ=255)

      aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                                >  1:3342403/1‑70 (MQ=255)

         tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatc                                                            <  1:2789595/71‑1 (MQ=255)

          cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                             >  1:1064608/1‑69 (MQ=255)

          cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                             >  1:2633924/1‑69 (MQ=255)

          cgcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                                             >  1:3066812/1‑69 (MQ=255)

          cgcgCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                                           >  1:3869491/1‑70 (MQ=255)

           gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                                           <  1:649728/70‑2 (MQ=255)

           gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                                                          >  1:3474994/1‑71 (MQ=255)

            cgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                                         <  1:213280/71‑1 (MQ=255)

              ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                       >  1:579528/1‑71 (MQ=255)

               tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                      >  1:3965443/1‑71 (MQ=255)

                gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                      <  1:2432739/70‑1 (MQ=255)

                gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                      <  1:2946684/70‑1 (MQ=255)

                gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                                                      <  1:3796741/70‑1 (MQ=255)

                  cGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                                                >  1:2060623/1‑58 (MQ=255)

                   gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                       >  1:515905/1‑66 (MQ=255)

                   gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATg                                                     <  1:2833312/68‑1 (MQ=255)

                   gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                                   <  1:743027/70‑1 (MQ=255)

                   gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGAc                                                   <  1:3300100/70‑1 (MQ=255)

                     atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                                <  1:1742926/71‑1 (MQ=255)

                     atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                                <  1:226263/71‑1 (MQ=255)

                     atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                                >  1:949823/1‑71 (MQ=255)

                     atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                                <  1:2747591/71‑1 (MQ=255)

                     atatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGt                                                <  1:972013/71‑1 (MQ=255)

                        tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa                                                    >  1:1157143/1‑64 (MQ=255)

                        tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                              >  1:4068641/1‑70 (MQ=255)

                        tGTTTTTGAATTAACTCCAGCAGCGACATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                              >  1:253024/1‑70 (MQ=255)

                         gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGa                                              >  1:2343386/1‑69 (MQ=255)

                          tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                                                       <  1:133888/43‑1 (MQ=255)

                          tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcatca                                                           >  1:96637/1‑55 (MQ=255)

                          tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                                <  1:492058/66‑1 (MQ=255)

                           ttctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGTAATatc                                          >  1:2935972/4‑71 (MQ=255)

                            tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                                         <  1:3571142/55‑1 (MQ=255)

                            tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                       >  1:805829/1‑57 (MQ=255)

                            tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                                          <  1:3514089/70‑1 (MQ=255)

                            tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                                          <  1:2776165/70‑1 (MQ=255)

                             ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                                                       >  1:2054602/1‑56 (MQ=255)

                             ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatca                                         <  1:1885155/70‑1 (MQ=255)

                              tGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                                       >  1:2162209/1‑71 (MQ=255)

                                 aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                                    <  1:2642815/71‑1 (MQ=255)

                                 aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                                    >  1:3365182/1‑71 (MQ=255)

                                 aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                                    >  1:3741100/1‑71 (MQ=255)

                                  ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                                         <  1:305933/49‑1 (MQ=255)

                                  ttAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGc                                   >  1:1876252/1‑71 (MQ=255)

                                     aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                                <  1:1013238/71‑1 (MQ=255)

                                     aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                                <  1:1965513/71‑1 (MQ=255)

                                         cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                                <  1:642744/51‑1 (MQ=255)

                                         cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                                <  1:92304/51‑1 (MQ=255)

                                         cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                                <  1:886151/67‑1 (MQ=255)

                                         cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                                <  1:940192/67‑1 (MQ=255)

                                         cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt                             <  1:1429735/70‑1 (MQ=255)

                                         cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt                             <  1:1845628/70‑1 (MQ=255)

                                           gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          <  1:1155648/71‑1 (MQ=255)

                                           gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          >  1:1354640/1‑71 (MQ=255)

                                           gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          <  1:2711612/71‑1 (MQ=255)

                                           gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          >  1:1790572/1‑71 (MQ=255)

                                           gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          >  1:2598025/1‑71 (MQ=255)

                                           gcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          >  1:591913/1‑71 (MQ=255)

                                             agcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGa                        <  1:1081708/71‑1 (MQ=255)

                                              gcgcCATACGTCGGTGCTGCATGATTTAATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg                       >  1:3446834/1‑71 (MQ=255)

                                               cgcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg                      <  1:1393371/71‑1 (MQ=255)

                                                gcCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAgg                      >  1:3236335/1‑70 (MQ=255)

                                                  cATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg                                               <  1:4143273/43‑1 (MQ=255)

                                                  cATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa                    >  1:524081/1‑70 (MQ=255)

                                                   aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatca                                     <  1:109437/52‑1 (MQ=255)

                                                   aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCATTCAGCGGAAATGCGGAGGCATa                  <  1:3300270/71‑1 (MQ=255)

                                                   aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          <  1:1348536/63‑1 (MQ=255)

                                                   aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt                   >  1:1469550/1‑70 (MQ=255)

                                                   aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCAt                   >  1:3647826/1‑70 (MQ=255)

                                                   aTACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATa                  <  1:873938/71‑1 (MQ=255)

                                                    tACGTCGGTGCTGCATGATTTCATCATCAGGGATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa                 <  1:753398/71‑1 (MQ=255)

                                                    tACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAg                       <  1:757004/65‑1 (MQ=255)

                                                     aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGc                           <  1:3208772/60‑1 (MQ=255)

                                                     aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          <  1:465720/61‑1 (MQ=255)

                                                     aCGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCa                    <  1:23824/67‑1 (MQ=255)

                                                      cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                                       >  1:1810413/1‑47 (MQ=255)

                                                      cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat                                       >  1:3225989/1‑47 (MQ=255)

                                                      cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGc                     >  1:424480/1‑65 (MQ=255)

                                                      cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAg               >  1:1005280/1‑71 (MQ=255)

                                                       gTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGc              >  1:654495/1‑71 (MQ=255)

                                                          ggTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTg            <  1:1940919/70‑1 (MQ=255)

                                                          ggTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTg            <  1:2016281/70‑1 (MQ=255)

                                                           gTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATg                            <  1:938548/53‑1 (MQ=255)

                                                            tgctgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCt         <  1:3207245/71‑1 (MQ=255)

                                                              ctgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaa                               <  1:3026034/47‑1 (MQ=255)

                                                              ctgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCtt        <  1:2148178/70‑1 (MQ=255)

                                                               tgcATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa                <  1:791204/61‑1 (MQ=255)

                                                                 cATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCTGAGGCa                    <  1:4031949/55‑1 (MQ=255)

                                                                 cATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTTGc      >  1:55463/1‑69 (MQ=255)

                                                                   tGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCg                          <  1:1526326/47‑1 (MQ=255)

                                                                   tGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTTGCCAGt  <  1:911134/71‑1 (MQ=255)

                                                                   tGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTTGCCAGt  >  1:2121385/1‑71 (MQ=255)

                                                                    gATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATg                            >  1:1104521/1‑44 (MQ=255)

CCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAAGCTGCCTTGCCAGT  >  minE/2677361‑2677498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`