| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,694,258 | C→T | 41.7% | E124K (GAA→AAA) | yrfF ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,694,258 | 0 | C | T | 41.7% | 49.5 / 100.6 | 84 | E124K (GAA→AAA) | yrfF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (23/26); new base T (23/12); total (46/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
AAATAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTAGA > minE/2694192‑2694327 | aaaTAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTc > 1:1892661/1‑67 (MQ=255) aaTAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGa < 1:2244884/71‑1 (MQ=255) aaTAACCAGCGGCAGCGAATCAGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGa < 1:812739/71‑1 (MQ=255) aTAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGa > 1:2809193/1‑70 (MQ=255) aTAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGa > 1:562708/1‑70 (MQ=255) aTAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGa > 1:97513/1‑70 (MQ=255) aaCCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAg > 1:753006/1‑71 (MQ=255) aCCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:528971/1‑69 (MQ=255) aCCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:1645815/1‑69 (MQ=255) aCCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaa > 1:2450331/1‑68 (MQ=255) aCCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAgg > 1:1982441/1‑71 (MQ=255) gCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAggggg > 1:1661255/1‑70 (MQ=255) ggCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAg < 1:3675182/71‑1 (MQ=255) gCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAgg < 1:244265/71‑1 (MQ=255) gCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAgg > 1:2736972/1‑71 (MQ=255) gCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGa < 1:2038554/60‑1 (MQ=255) gCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAgg > 1:666811/1‑71 (MQ=255) aGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTg < 1:1340754/71‑1 (MQ=255) aGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTg < 1:2154592/71‑1 (MQ=255) gCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTg > 1:990529/1‑70 (MQ=255) cGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGa > 1:2059313/1‑71 (MQ=255) gAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGa > 1:1591368/1‑70 (MQ=255) gAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGAc > 1:3416137/1‑71 (MQ=255) aaTCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACt > 1:3597109/1‑71 (MQ=255) aTCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAg > 1:1732298/1‑56 (MQ=255) aTCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAg > 1:686939/1‑56 (MQ=255) tCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAggggg < 1:638844/59‑1 (MQ=255) ggTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTc > 1:742373/1‑45 (MQ=255) ggTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTa > 1:3414894/1‑71 (MQ=255) tATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAATGGGGCAGGTGGACTTCTAcc < 1:3028824/71‑1 (MQ=255) tGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaa < 1:3478996/47‑1 (MQ=255) tGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCg < 1:3305551/70‑1 (MQ=255) aaTCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAAt > 1:840066/1‑71 (MQ=255) cAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCg < 1:385438/70‑1 (MQ=255) aGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:1049634/1‑42 (MQ=255) aGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:1287041/1‑42 (MQ=255) aGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:734605/1‑42 (MQ=255) aGTTCAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAg > 1:2001489/1‑71 (MQ=255) aGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCa > 1:1021109/1‑38 (MQ=255) gTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCg < 1:3591184/63‑1 (MQ=255) gTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAgg < 1:3974459/71‑1 (MQ=255) gTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAgg < 1:3834560/71‑1 (MQ=255) ttCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAgg < 1:3138413/42‑1 (MQ=255) gaaCGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgtag > 1:3042192/2‑71 (MQ=255) cAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAggg > 1:1322733/1‑41 (MQ=255) cAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGAc > 1:3159500/1‑52 (MQ=255) cAACGGTATTCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgta < 1:505403/70‑1 (MQ=255) cAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAgg > 1:3258748/1‑68 (MQ=255) cAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgtag > 1:1337984/1‑71 (MQ=255) aaCGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCg < 1:3595948/59‑1 (MQ=255) cGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:2470081/1‑35 (MQ=255) cGGTATTCTCATCGTTGATGTACTGTTTCCAGaaa > 1:3785055/1‑35 (MQ=255) ttCTCATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgtagta < 1:326741/65‑1 (MQ=255) ttCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGa < 1:1539968/53‑1 (MQ=255) ctcATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCAtt > 1:3413063/1‑71 (MQ=255) ctcATCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCAtt > 1:2562158/1‑71 (MQ=255) ctcATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgt > 1:3162253/1‑59 (MQ=255) ctcATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgt > 1:1000358/1‑59 (MQ=255) ctcATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCAtt > 1:143239/1‑71 (MQ=255) tcATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTaa > 1:3089855/1‑63 (MQ=255) aTCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGa < 1:3947122/48‑1 (MQ=255) aTCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTa < 1:2052324/70‑1 (MQ=255) aTCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTAt > 1:1039223/1‑71 (MQ=255) tCGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTAtt > 1:1201338/1‑71 (MQ=255) tCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTAtt < 1:354524/71‑1 (MQ=255) cGTTGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTc > 1:1936108/1‑71 (MQ=255) gTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTc < 1:3688976/70‑1 (MQ=255) gTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGAAGTAACGCCATTTATTc < 1:3828716/70‑1 (MQ=255) ttGATGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCg < 1:622119/70‑1 (MQ=255) gATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGa < 1:3997730/70‑1 (MQ=255) tGTACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGa < 1:3110362/40‑1 (MQ=255) tGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGa > 1:36979/1‑45 (MQ=255) tGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTaa < 1:2768851/53‑1 (MQ=255) gTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATc < 1:3381061/42‑1 (MQ=255) tACTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGAtcgtc > 1:2575376/1‑71 (MQ=255) tACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgta < 1:2838418/47‑1 (MQ=255) aCTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGAtc > 1:1216741/1‑67 (MQ=255) aCTGTTCCCAGAAAGGGGGCAGGTGGACTTATACCGAATCGAGGTAGTAACGCCATTTATTCGGAtcgtcg > 1:2565569/1‑71 (MQ=255) cTGTTTCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCgg < 1:2793854/71‑1 (MQ=255) cTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCgg < 1:2289528/71‑1 (MQ=255) cTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCgg > 1:1537991/1‑71 (MQ=255) cTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCgg < 1:392486/71‑1 (MQ=255) ttCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGgt < 1:556490/41‑1 (MQ=255) ttCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTag < 1:647874/71‑1 (MQ=255) ttCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTag < 1:3876857/71‑1 (MQ=255) ttCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTa < 1:926584/70‑1 (MQ=255) cccATAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTaga < 1:2848583/70‑1 (MQ=255) cccAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTaga < 1:3938370/70‑1 (MQ=255) | AAATAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTATTCTCATCGTTGATGTACTGTTCCCAGAAAGGGGGCAGGTGGACTTCTACCGAATCGAGGTAGTAACGCCATTTATTCGGATCGTCGGTAGA > minE/2694192‑2694327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |