| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,708,913 | G→A | 22.2% | E54E (GAG→GAA) | yhfZ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,708,913 | 0 | G | A | 22.2% | 207.6 / 54.2 | 99 | E54E (GAG→GAA) | yhfZ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (34/43); new base A (12/10); total (46/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
ATAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGAA > minE/2708845‑2708981 | aTAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGt > 1:146153/1‑70 (MQ=255) tAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGt > 1:2950888/1‑69 (MQ=255) tAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTc > 1:4109908/1‑70 (MQ=255) tAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCa < 1:1860611/71‑1 (MQ=255) aGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCgg < 1:205267/70‑1 (MQ=255) gCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAg < 1:105933/66‑1 (MQ=255) cTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGc < 1:2570202/71‑1 (MQ=255) gCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCg > 1:3155246/1‑69 (MQ=255) gCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGa > 1:1515502/1‑70 (MQ=255) aaaTGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATAc > 1:2706802/1‑71 (MQ=255) aatgaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACg < 1:475255/71‑1 (MQ=255) aatgaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACg < 1:2739228/71‑1 (MQ=255) tgaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGa > 1:3283824/1‑71 (MQ=255) gaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGAAACGGAt > 1:966581/1‑71 (MQ=255) aatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCaa > 1:2382129/1‑54 (MQ=255) atgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTg < 1:3878530/71‑1 (MQ=255) gTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCgg < 1:3833675/52‑1 (MQ=255) ttCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGcc < 1:1176151/71‑1 (MQ=255) tCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGcc > 1:250537/1‑70 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGGGGAGCGATACGGATTGAACGCCGt > 1:1756292/1‑71 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGt > 1:3724457/1‑71 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGa > 1:1375940/1‑60 (MQ=255) aTCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGAt < 1:1284677/54‑1 (MQ=255) aTCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGAt < 1:788844/54‑1 (MQ=255) tCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGAtt < 1:1509512/60‑1 (MQ=255) ccGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTg < 1:2214552/60‑1 (MQ=255) cGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTgg > 1:2672779/1‑69 (MQ=255) cGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgc > 1:31357/1‑71 (MQ=255) cGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgc > 1:2903755/1‑71 (MQ=255) cGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgc > 1:3725484/1‑71 (MQ=255) cGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGaa > 1:2909924/1‑61 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:2040263/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:3154134/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:3677519/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:2259726/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGGGGgcgc < 1:36450/71‑1 (MQ=255) ggCCTGACGCAGGCGGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCaa > 1:1504774/1‑71 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTc > 1:557081/1‑35 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGa > 1:1943040/1‑52 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCaa > 1:1253655/1‑71 (MQ=255) gCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACg < 1:3026154/49‑1 (MQ=255) gCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAAt < 1:3407387/71‑1 (MQ=255) ccTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATg < 1:2462936/71‑1 (MQ=255) cTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGAtt > 1:923096/1‑51 (MQ=255) tGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATgg > 1:1916551/1‑70 (MQ=255) tGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATgg > 1:2629406/1‑70 (MQ=255) gACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATgg > 1:4048491/1‑69 (MQ=255) aCGCTGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:1392994/63‑1 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCa < 1:444106/70‑1 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAg < 1:1094105/71‑1 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCa < 1:3040757/70‑1 (MQ=255) cGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATg > 1:1904813/1‑66 (MQ=255) cGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:1601438/1‑71 (MQ=255) cGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt < 1:273320/71‑1 (MQ=255) gCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt < 1:3598109/70‑1 (MQ=255) aGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTAt < 1:2904745/71‑1 (MQ=255) ggCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATc < 1:809831/71‑1 (MQ=255) ggCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATc < 1:3741221/71‑1 (MQ=255) gCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATc > 1:888524/1‑70 (MQ=255) ccGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCaa > 1:1421141/1‑58 (MQ=255) ccGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTg > 1:1558413/1‑71 (MQ=255) cgcgTTGAAAACGCTGGAGTCAGGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTgg > 1:3666824/1‑71 (MQ=255) cgcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTAt > 1:386572/1‑67 (MQ=255) gcgTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGt > 1:2201822/1‑71 (MQ=255) gcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATc < 1:2943590/67‑1 (MQ=255) gTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCg < 1:2222932/71‑1 (MQ=255) gTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTc > 1:1990978/1‑70 (MQ=255) gTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTc > 1:437269/1‑70 (MQ=255) gTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTc > 1:36353/1‑70 (MQ=255) tGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGtt < 1:2294338/60‑1 (MQ=255) gAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:3785943/49‑1 (MQ=255) gAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATc > 1:2404091/1‑62 (MQ=255) gAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg > 1:4018181/1‑70 (MQ=255) gAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg > 1:3002208/1‑70 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTgg > 1:3916998/1‑65 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg < 1:2620358/70‑1 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg < 1:621377/70‑1 (MQ=255) gAAAACGATGGAGTCAAGCGGAGCGATACGGATTGAACg > 1:190533/1‑39 (MQ=255) aaaaCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGa < 1:1524085/71‑1 (MQ=255) aaaaCGCTCGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGa < 1:1311578/71‑1 (MQ=255) aaaCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTAt > 1:1104349/1‑59 (MQ=255) aaaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAgat < 1:637390/70‑3 (MQ=255) aaCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGaa > 1:4142287/1‑70 (MQ=255) aaCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAat < 1:3416209/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:2944265/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:628937/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:1681288/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:2505037/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:1073749/71‑1 (MQ=255) cGCTGGAGTCAAGCGTAGCGATACGGATTGAACACCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAatat < 1:3900053/71‑1 (MQ=255) cGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAatat < 1:3762785/71‑1 (MQ=255) cGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAatat < 1:455362/71‑1 (MQ=255) cGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAatat < 1:3644871/71‑1 (MQ=255) cGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAatat < 1:3087029/71‑1 (MQ=255) gCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTgg < 1:169084/39‑1 (MQ=255) tGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATAc > 1:62294/1‑70 (MQ=255) gAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACg > 1:2372440/1‑69 (MQ=255) gAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGaa < 1:1140455/71‑1 (MQ=255) gAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGaa < 1:289195/71‑1 (MQ=255) | ATAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGAA > minE/2708845‑2708981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |