| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,748,276 | A→G | 13.2% | S117G (AGC→GGC) | secY → | preprotein translocase membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,748,276 | 0 | A | G | 13.2% | 176.5 / 18.0 | 68 | S117G (AGC→GGC) | secY | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base A (32/27); new base G (6/3); total (38/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CTGACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGTCTGC > minE/2748210‑2748345 | ctgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGc < 1:3632677/69‑1 (MQ=255) tgACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc > 1:2396993/1‑68 (MQ=255) gACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg > 1:2978706/1‑70 (MQ=255) cGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg < 1:2057322/68‑1 (MQ=255) cGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTac < 1:539045/71‑1 (MQ=255) ggtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGcc < 1:2340247/65‑1 (MQ=255) ggtggtTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTac < 1:2967821/70‑1 (MQ=255) gtggtTAACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTacac < 1:1062179/71‑1 (MQ=255) ttCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGc < 1:3164683/59‑1 (MQ=255) ttCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg < 1:2384452/62‑1 (MQ=255) cACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTa > 1:1002815/1‑71 (MQ=255) cACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTa > 1:1975001/1‑71 (MQ=255) cccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACg > 1:2656171/1‑71 (MQ=255) ccAACGTTGGCATAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACgg < 1:810896/71‑1 (MQ=255) ccAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACgg > 1:2657072/1‑71 (MQ=255) aCGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCACTACACCCGCTACGGTAc > 1:3195060/1‑71 (MQ=255) gTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTActc > 1:534881/1‑71 (MQ=255) ttGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCa > 1:1821289/1‑50 (MQ=255) ttGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCGGCCa > 1:2137591/1‑50 (MQ=255) ttGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg < 1:1633777/51‑1 (MQ=255) ttGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTActct < 1:3297872/71‑1 (MQ=255) ttGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTActct > 1:3315979/1‑71 (MQ=255) ggCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTgg > 1:3945134/1‑71 (MQ=255) gCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGc < 1:770055/45‑1 (MQ=255) gCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGc < 1:2379727/45‑1 (MQ=255) gAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg < 1:1034960/45‑1 (MQ=255) aaaTTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTg > 1:1641247/1‑71 (MQ=255) aaTTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg > 1:1360029/1‑43 (MQ=255) aaTTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAg > 1:2831571/1‑43 (MQ=255) aagaaagaAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa < 1:364418/70‑1 (MQ=255) agaaagaaGGGGAGTCTGGTCGTCGTAAGATCAGc < 1:3485145/35‑1 (MQ=255) agaagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGc < 1:2229779/63‑1 (MQ=255) agaagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTc > 1:3507060/1‑70 (MQ=255) agaagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTc > 1:1997679/1‑70 (MQ=255) agaagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTAAACCCGCTACGGTACTCTgg > 1:2896881/1‑56 (MQ=255) aagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg > 1:3582700/1‑71 (MQ=255) aagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg > 1:2255660/1‑71 (MQ=255) aagGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAg > 1:2779774/1‑71 (MQ=255) cggggAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGc < 1:345509/59‑1 (MQ=255) agGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAata < 1:76315/64‑1 (MQ=255) ggggAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTc > 1:2724940/1‑71 (MQ=255) gggAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGc < 1:163418/38‑1 (MQ=255) gggAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCg < 1:2814779/71‑1 (MQ=255) gggAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCg < 1:2225973/71‑1 (MQ=255) gggAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCg < 1:1899008/71‑1 (MQ=255) tCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAatat < 1:186347/58‑1 (MQ=255) tCTGGTCGTCGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAatat < 1:3059902/58‑1 (MQ=255) tCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTg < 1:2574092/48‑1 (MQ=255) tCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCgg < 1:834474/71‑1 (MQ=255) tGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCa > 1:109416/1‑60 (MQ=255) gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATAtt > 1:863251/1‑55 (MQ=255) gtcgtcGTAAGATCGGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTa > 1:2238774/1‑69 (MQ=255) gtcgtcGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCg < 1:2487941/62‑1 (MQ=255) tcgtcgTAAGATCAGCCAGTACACCCGCTACGGTActc < 1:997474/38‑1 (MQ=255) gtcgtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGt > 1:1471062/1‑40 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCaa > 1:2580474/1‑45 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAata > 1:191803/1‑47 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTa > 1:3271658/1‑63 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAcc > 1:1424302/1‑71 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAcc > 1:1382923/1‑71 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAcc > 1:1378169/1‑71 (MQ=255) gtAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTAcc > 1:2864117/1‑71 (MQ=255) tAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCg < 1:2456347/71‑1 (MQ=255) aaGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCgg > 1:3575348/1‑71 (MQ=255) aGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGt > 1:1809753/1‑71 (MQ=255) tCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCt > 1:3045089/1‑62 (MQ=255) tCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGTCTg > 1:371118/1‑71 (MQ=255) cAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGTCTGc > 1:378427/1‑71 (MQ=255) | CTGACGGTGGTTCACCCAACGTTGGCAGAAATTAAGAAAGAAGGGGAGTCTGGTCGTCGTAAGATCAGCCAGTACACCCGCTACGGTACTCTGGTGCTGGCAATATTCCAGTCGATCGGTATTGCTACCGGTCTGC > minE/2748210‑2748345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |