| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,529 | T→C | 5.3% | V448V (GTA→GTG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,529 | 0 | T | C | 5.3% | 278.0 / 5.6 | 95 | V448V (GTA→GTG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (52/38); new base C (3/2); total (55/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCA > minE/311461‑311599 | gagaACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAc > 1:2879698/1‑71 (MQ=9) agaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc < 1:2908535/71‑1 (MQ=255) cTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATcg > 1:3074943/1‑71 (MQ=255) gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc > 1:2217854/1‑70 (MQ=255) gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc > 1:2164353/1‑70 (MQ=255) gcGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa < 1:57355/70‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:2122659/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:135907/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3675328/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:2649993/69‑1 (MQ=255) agatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc > 1:203348/1‑70 (MQ=255) atagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:440967/62‑1 (MQ=255) atagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:136807/62‑1 (MQ=255) agCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAc > 1:1004187/1‑69 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2320443/1‑61 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:3208573/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:1857996/71‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCag < 1:304715/71‑1 (MQ=255) aCCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc < 1:1521534/52‑1 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAcc > 1:3144201/1‑49 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc > 1:81596/1‑48 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:3139594/1‑71 (MQ=255) tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3356690/52‑1 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:3841881/1‑70 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:1238850/1‑71 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3635217/50‑1 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2131698/1‑52 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa > 1:551963/1‑61 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:3752865/1‑69 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3172011/1‑70 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:3295307/1‑70 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:1065092/1‑70 (MQ=255) aaCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:2121909/71‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:2506293/70‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:907978/70‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:307462/70‑1 (MQ=255) ccGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:2925843/1‑64 (MQ=255) ccGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:3389224/1‑64 (MQ=255) ccGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:3394083/1‑64 (MQ=255) cGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc < 1:2007500/71‑1 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc > 1:2684275/1‑39 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa > 1:1976328/1‑68 (MQ=255) cAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCAt > 1:859337/1‑40 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:917748/1‑62 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:1683033/64‑1 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTccc < 1:1776318/71‑1 (MQ=255) aagaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAc > 1:2206760/1‑50 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc > 1:3513594/1‑46 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc > 1:2018142/1‑46 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:1153421/51‑1 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGaa < 1:2970877/63‑1 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:718917/1‑40 (MQ=255) tgCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:1810850/70‑1 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:3198546/71‑1 (MQ=255) gCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:3344247/35‑1 (MQ=255) gCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCACATCGa < 1:942695/71‑1 (MQ=255) ccATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCaga > 1:3116835/1‑47 (MQ=255) ccATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGat > 1:3998371/1‑70 (MQ=255) cATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc < 1:1901301/52‑1 (MQ=255) cATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:282216/1‑53 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:2053496/1‑51 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:3682092/1‑51 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:2069669/71‑1 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:3627637/71‑1 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt > 1:1312938/1‑70 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc > 1:2068817/1‑54 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:2697424/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:3798475/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:3190938/1‑71 (MQ=255) aCGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg > 1:2551911/1‑70 (MQ=255) aCGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:2472916/1‑68 (MQ=255) gAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:3716955/59‑1 (MQ=255) aaTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc > 1:1409621/1‑48 (MQ=255) aaTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGa > 1:2374307/1‑59 (MQ=255) aaTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACtt < 1:3926985/62‑1 (MQ=255) aaTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGc > 1:3307058/1‑71 (MQ=255) aaTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGc > 1:65617/1‑71 (MQ=255) aTACCGCCGACAGTACCATCGCGATACCGCCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGct > 1:24267/1‑71 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:131555/43‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCCTCGACTTACGGGTAg > 1:3256523/1‑68 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGct > 1:3960291/1‑70 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAAt > 1:370888/1‑43 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttct < 1:3643074/71‑1 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttct < 1:3223678/71‑1 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc < 1:326535/70‑1 (MQ=255) gccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCttt > 1:902879/1‑71 (MQ=255) ccgACAGTACCATCGCGATATCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:404071/1‑56 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:2314051/1‑56 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:3809441/1‑56 (MQ=255) ccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:366257/1‑56 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt < 1:82532/70‑1 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt < 1:834924/70‑1 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt < 1:3426185/70‑1 (MQ=255) cgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTg < 1:2786649/71‑1 (MQ=255) cAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcg > 1:114347/1‑71 (MQ=255) tACCATCGCGATACCGACCAGAGCGCCCTGAATCTACCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCa < 1:3499010/71‑1 (MQ=255) | GAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCA > minE/311461‑311599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |