| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,792,242 | C→T | 38.0% | T118I (ACC→ATC) | ubiA → | p‑hydroxybenzoate octaprenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,792,242 | 0 | C | T | 38.0% | 71.6 / 83.9 | 79 | T118I (ACC→ATC) | ubiA | p‑hydroxybenzoate octaprenyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (32/17); new base T (16/14); total (48/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCT > minE/2792175‑2792311 | gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCAt < 1:1329881/71‑1 (MQ=255) gcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCtg > 1:2239041/1‑71 (MQ=255) gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCAt < 1:3193442/65‑1 (MQ=255) gcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCtgttgt < 1:1601927/71‑1 (MQ=255) tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCtgttgt > 1:2511165/1‑69 (MQ=255) tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTc > 1:2563368/1‑70 (MQ=255) tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCt < 1:3890691/62‑1 (MQ=255) tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCg < 1:84106/69‑1 (MQ=255) tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCg < 1:2918852/69‑1 (MQ=255) ttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGAcc < 1:3520629/56‑1 (MQ=255) ttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCa > 1:429520/1‑57 (MQ=255) gTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAgc > 1:4074660/1‑71 (MQ=255) aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAgcgc > 1:3708224/1‑71 (MQ=255) aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAgcgc > 1:546495/1‑71 (MQ=255) aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAg > 1:4039783/1‑68 (MQ=255) cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGa > 1:3918082/1‑55 (MQ=255) cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTc > 1:3375641/1‑46 (MQ=255) cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAgcgc < 1:865090/70‑1 (MQ=255) cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCt > 1:2593891/1‑71 (MQ=255) cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCt > 1:3395797/1‑71 (MQ=255) gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGa > 1:1045068/1‑53 (MQ=255) acttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGa > 1:2191128/3‑52 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTc > 1:3843228/1‑43 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCgcgc > 1:158579/1‑61 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCgcgc > 1:675237/1‑61 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGa > 1:3169847/1‑52 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCt > 1:3550586/1‑68 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTgg < 1:4138066/70‑1 (MQ=255) aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATCCTGTTGTCGATTGCCGCGCTAGCGCTgg < 1:893493/70‑1 (MQ=255) tttCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTc < 1:2977406/49‑1 (MQ=255) tttCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTc < 1:3099614/49‑1 (MQ=255) tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCg < 1:621737/50‑1 (MQ=255) ttCGTTTTTACTGGTGCTGACGCTGAATACGATGATCAt < 1:2817630/39‑1 (MQ=255) ttCGTTTTTACTGGTGCTGACGCTGAATACGATGATCAt < 1:3693721/39‑1 (MQ=255) ttCGTTTTTACTGGTGCTGACCCTGAATACGATGACCATTCTGTTGTCGa > 1:3143047/1‑50 (MQ=255) tCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGa > 1:1170710/1‑49 (MQ=255) tCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGcc > 1:1863092/1‑54 (MQ=255) tCGTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGc < 1:2178955/53‑1 (MQ=255) tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCtgt > 1:2636958/1‑43 (MQ=255) tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCtgt > 1:3529102/1‑43 (MQ=255) tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGAt > 1:1046245/1‑50 (MQ=255) cGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAg < 1:1980400/60‑1 (MQ=255) gTTTTTACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTAGa > 1:275610/1‑47 (MQ=38) tttttACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt < 1:554663/67‑1 (MQ=255) tttttACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt < 1:554717/67‑1 (MQ=255) tttACTGGTGCTGACGCTGAATACGATGATCATTCtgtt > 1:422831/1‑39 (MQ=255) tttACTGGTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAgc < 1:1584852/57‑1 (MQ=255) tttACTGGTGCTGACGCTGAATACGATGACCATTCtgt > 1:354845/1‑38 (MQ=255) gTGCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGtt > 1:2996428/1‑71 (MQ=255) gTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGtt > 1:1067605/1‑71 (MQ=255) gTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGtt > 1:722056/1‑71 (MQ=255) gTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGtt > 1:570782/1‑71 (MQ=255) tGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCg < 1:479589/56‑1 (MQ=255) cgCTGGCGCTGAATACGATGACCATTCTGTTGTCGATTGcc > 1:2699063/2‑41 (MQ=255) gCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTggg > 1:1629527/1‑59 (MQ=255) gCTGACGCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc < 1:3959411/64‑1 (MQ=255) gCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt > 1:1192006/1‑56 (MQ=255) gCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg > 1:1427386/1‑61 (MQ=255) cTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAgc < 1:1696680/47‑1 (MQ=255) cTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgt > 1:3125004/1‑59 (MQ=255) cTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt > 1:1765735/1‑71 (MQ=255) cTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt > 1:2912339/1‑71 (MQ=255) aCGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa < 1:1438867/70‑1 (MQ=255) gCTGAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc < 1:1802686/71‑1 (MQ=255) gCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc > 1:2745277/1‑71 (MQ=255) gAATACGATGATCATTCTGTTGTCGATTGCCGCGCTa > 1:1576547/1‑37 (MQ=255) gAATACGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg > 1:3241632/1‑52 (MQ=255) aaTACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg < 1:749084/69‑1 (MQ=255) aaTACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGta > 1:2973190/1‑71 (MQ=255) aTACGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGta > 1:2726191/1‑70 (MQ=255) aTACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg < 1:2568756/68‑1 (MQ=255) tACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTgg > 1:3614047/1‑46 (MQ=255) tACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTgg > 1:1642171/1‑46 (MQ=255) cGATGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg < 1:1276386/65‑1 (MQ=255) cGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGAGTGGGTGTACCCGTTTATGAAGCGGTATAca > 1:3995939/1‑70 (MQ=255) aTGATCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCa < 1:1567026/71‑1 (MQ=255) aTGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAccc > 1:328508/1‑50 (MQ=255) aCCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCg < 1:1298472/48‑1 (MQ=255) aCCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCt < 1:1019839/71‑1 (MQ=255) aCCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCt < 1:2290864/71‑1 (MQ=255) | GCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCT > minE/2792175‑2792311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |