| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,823,628 | G→A | 12.6% | intergenic (+373/+585) | nrfG → / ← pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,823,628 | 0 | G | A | 12.6% | 289.9 / 26.3 | 103 | intergenic (+373/+585) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base G (61/29); new base A (6/7); total (67/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATG > minE/2823563‑2823694 | cGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCgatgat < 1:3150336/71‑1 (MQ=255) ggCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCg < 1:467427/58‑1 (MQ=255) cgcTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCcac > 1:3714782/1‑70 (MQ=255) cgcTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCcac > 1:2420438/1‑70 (MQ=255) ccATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAAcc > 1:3938496/1‑70 (MQ=255) cATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCg < 1:2255143/51‑1 (MQ=255) cATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTa > 1:2394670/1‑71 (MQ=255) ttGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATaa > 1:54233/1‑56 (MQ=255) ttGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAtt > 1:2463536/1‑71 (MQ=255) ttGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAtt > 1:853448/1‑71 (MQ=255) ttGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAtt > 1:4075233/1‑71 (MQ=255) tGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAttt > 1:230719/1‑71 (MQ=255) tGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAtt > 1:1520810/1‑70 (MQ=255) gAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATaa > 1:812392/1‑54 (MQ=255) gAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAtttt > 1:2013470/1‑71 (MQ=255) gAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAttt > 1:2165621/1‑70 (MQ=255) aGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAttttt < 1:3830551/71‑1 (MQ=255) gAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTAc > 1:1926336/1‑71 (MQ=255) gTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCAt < 1:2640135/71‑1 (MQ=255) tcattcatAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCAt > 1:2241750/1‑70 (MQ=255) cattcatAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAt > 1:913245/1‑60 (MQ=255) ttcatAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAAtt < 1:2092902/46‑1 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCgat > 1:1155329/1‑37 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCgat > 1:2899427/1‑37 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCga > 1:4040529/1‑36 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:1685532/70‑1 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:3386912/70‑1 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:623330/70‑1 (MQ=255) catAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:1392420/70‑1 (MQ=255) ataataGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCta > 1:4006042/1‑67 (MQ=255) ataataGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAg < 1:3692205/70‑1 (MQ=255) taataGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:3215030/68‑1 (MQ=255) aataGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATg > 1:1247454/1‑71 (MQ=255) ataGAAACTCATTCGAAAAGGGAATGATGCgatgat < 1:1624188/36‑1 (MQ=255) ataGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGa > 1:2743956/1‑68 (MQ=255) ataGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATgg > 1:1322619/1‑71 (MQ=255) ataGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATgg > 1:2132685/1‑71 (MQ=255) aGAAACTCATTCGAAAAGGGAATGATGCGATGATaa > 1:2838755/1‑36 (MQ=255) aGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAtttt > 1:199414/1‑53 (MQ=255) aGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCta > 1:3073178/1‑62 (MQ=255) aGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGt > 1:2762139/1‑71 (MQ=255) gAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAt > 1:2533659/1‑49 (MQ=255) gAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGa > 1:2829930/1‑65 (MQ=255) aaaCTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTa < 1:3595352/53‑1 (MQ=255) aaaCTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGAt > 1:453655/1‑65 (MQ=255) aaaCTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGttt < 1:3300544/71‑1 (MQ=255) aaCTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGttta < 1:2680768/71‑1 (MQ=255) aCTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTAcc > 1:2285278/1‑53 (MQ=255) aCTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTAATTTTACCATCTATAGATGGGtttat > 1:3574128/1‑71 (MQ=255) cTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:4126313/59‑1 (MQ=255) cTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttatt < 1:3113606/71‑1 (MQ=255) cTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATc < 1:2603234/55‑1 (MQ=255) tCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGAt < 1:2912635/61‑1 (MQ=255) tCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttatt > 1:3366720/1‑70 (MQ=255) cATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata > 1:260142/1‑57 (MQ=255) cATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttattta > 1:2067535/1‑71 (MQ=255) cATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttatt > 1:2206066/1‑69 (MQ=255) cATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttattta > 1:1872913/1‑71 (MQ=255) aTTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTAc > 1:3173241/1‑48 (MQ=255) aTTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCAt > 1:4064047/1‑51 (MQ=255) aTTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:3840847/56‑1 (MQ=255) aTTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCtata < 1:2609374/56‑1 (MQ=255) aTTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttattta < 1:637196/70‑1 (MQ=255) aTTCGAAAAGGGAATGATGCAATGATAATTGCCACAAcc < 1:575915/39‑1 (MQ=255) ttCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACa > 1:3196579/1‑71 (MQ=255) ttCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACa > 1:2074747/1‑71 (MQ=255) cGAAAAGGGAATGATGCGATGATAATTGCCACAACCt > 1:2369353/1‑37 (MQ=255) cGAAAAGGGAATGATGCGATGATAATTGCCACAACCTa > 1:507456/1‑38 (MQ=255) cGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAg > 1:684721/1‑54 (MQ=255) cGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACata > 1:3879769/1‑71 (MQ=255) cGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACata > 1:3570980/1‑71 (MQ=255) cGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACata > 1:498682/1‑71 (MQ=255) gAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACatat < 1:2422398/71‑1 (MQ=255) aaaaGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATc < 1:1612339/47‑1 (MQ=255) aaaGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACAtatta > 1:903100/1‑71 (MQ=255) aaaGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACAtatt > 1:2392906/1‑70 (MQ=255) aaaGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACAtatt > 1:2397561/1‑70 (MQ=255) aaGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGttt < 1:1354094/58‑1 (MQ=255) aGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTATATattatt < 1:905151/71‑1 (MQ=255) aGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATattatt < 1:2692151/71‑1 (MQ=255) aGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATattatt < 1:1890416/71‑1 (MQ=255) gggAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTg < 1:1482875/71‑1 (MQ=255) ggAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTgg > 1:115361/1‑71 (MQ=255) aaTGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACat > 1:1075386/1‑61 (MQ=255) aaTGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACat > 1:1493213/1‑61 (MQ=255) aaTGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTg < 1:2570664/71‑1 (MQ=255) aaTGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTg < 1:1478107/71‑1 (MQ=255) aaTGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTg < 1:1880529/71‑1 (MQ=255) atgatgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttattta > 1:3793422/1‑57 (MQ=255) tgatgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGtttatt < 1:1116996/54‑1 (MQ=255) gatgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGt > 1:2689650/1‑48 (MQ=255) gatgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGt < 1:1822740/67‑1 (MQ=255) atgCGATGATAATTGCCACAACCTATTTTTACCATCtata > 1:3353504/1‑40 (MQ=255) atgCGATGATAATTGCCACAACCTATTTTTACCATCtata > 1:1020016/1‑40 (MQ=255) atgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGa > 1:1969754/1‑42 (MQ=255) atgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACAtatta > 1:2861199/1‑61 (MQ=255) atgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATg > 1:3884283/1‑71 (MQ=255) tgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATattatt > 1:2422957/1‑62 (MQ=255) tgCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGaa > 1:1324286/1‑68 (MQ=255) tgCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATg > 1:2195709/1‑70 (MQ=255) | CGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGCGATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATG > minE/2823563‑2823694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |