| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,823,871 | A→G | 34.9% | intergenic (+616/+342) | nrfG → / ← pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,823,871 | 0 | A | G | 34.9% | 60.4 / 62.2 | 63 | intergenic (+616/+342) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base A (15/26); new base G (11/11); total (26/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
AAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGT > minE/2823811‑2823938 | aaaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTg < 1:3252171/71‑1 (MQ=255)aaaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTg < 1:2785730/71‑1 (MQ=255) aaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGc < 1:3122313/71‑1 (MQ=255) cAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGc > 1:2897820/1‑70 (MQ=255) aGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCa < 1:2670419/70‑1 (MQ=255) aaaTAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCg < 1:1632918/71‑1 (MQ=255) aataatCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGa < 1:1813912/71‑1 (MQ=255) tGGAAAATTGTCCTGAGCACTTACTATTTTAATGGATTATTAGTc < 1:2068250/45‑1 (MQ=255) gAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACAtt > 1:935496/1‑71 (MQ=255) gAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACAtt > 1:4105579/1‑71 (MQ=255) gAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACAtt > 1:1257557/1‑71 (MQ=255) gAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGa > 1:2310077/1‑52 (MQ=255) gAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACAtt > 1:113030/1‑71 (MQ=255) gAAAATAGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGa > 1:539873/1‑52 (MQ=255) aTTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTtctc < 1:1487202/71‑1 (MQ=255) tGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCtt > 1:297748/1‑71 (MQ=255) gTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCAt > 1:3226369/1‑43 (MQ=255) tCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTg < 1:3493600/71‑1 (MQ=255) ccGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTg > 1:1625107/1‑70 (MQ=255) cGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGa > 1:3962235/1‑42 (MQ=255) cGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCttt < 1:3095038/68‑1 (MQ=255) cGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGtt > 1:1469610/1‑71 (MQ=255) ggAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCtt < 1:2916013/66‑1 (MQ=255) ggAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGa < 1:3023725/49‑1 (MQ=255) ggAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTtc < 1:1032707/71‑1 (MQ=255) gAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCAt < 1:3026155/38‑1 (MQ=255) gAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTtct < 1:4023132/71‑1 (MQ=255) gCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCa > 1:1206669/1‑71 (MQ=255) cTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGaa > 1:3119468/1‑44 (MQ=255) ttACTATTTTAATGGATTGTTAGTCTTTGCATGAGCa < 1:2847175/37‑1 (MQ=255) ttACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAgag < 1:1695497/41‑3 (MQ=255) ttACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCg < 1:1115706/41‑1 (MQ=255) ttACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCAtt > 1:2451593/1‑69 (MQ=255) ttACTATTTTAATGGATTATTAGTCTTTGCATGAGCa < 1:1287468/37‑1 (MQ=255) ttACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGa < 1:2621874/47‑1 (MQ=255) ttACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGa < 1:360704/47‑1 (MQ=255) ttACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGa < 1:1945963/47‑1 (MQ=255) tACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCtt < 1:2306150/58‑1 (MQ=255) aCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCa > 1:704261/1‑69 (MQ=255) aCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAg > 1:3155230/1‑70 (MQ=255) aCTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAg > 1:1938506/1‑70 (MQ=255) aCTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAg > 1:696225/1‑70 (MQ=255) cTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGa > 1:4091320/1‑44 (MQ=255) cTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGa > 1:253670/1‑44 (MQ=255) aTTTTAATGGATTATTAGTCTTTGCATGAGCAAGCg < 1:2258078/36‑1 (MQ=255) ttttAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTctct < 1:544870/52‑1 (MQ=255) ttttAATGGATTATTAGTCTTTGCATGAGCAAGCg < 1:1111254/35‑1 (MQ=255) ttttAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACAt < 1:2225576/46‑1 (MQ=255) ttttAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGaaa < 1:342800/69‑1 (MQ=255) ttttAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGaaa < 1:4101150/69‑1 (MQ=255) ttAATGGATTGTTAGTCTTTGCATGAGCAAGCTaa > 1:2423822/1‑35 (MQ=38) tAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGaaaa > 1:3747606/1‑67 (MQ=255) tAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtct < 1:2793272/70‑1 (MQ=255) caTGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACAttt > 1:1747821/2‑44 (MQ=255) aaTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtctc < 1:2343694/70‑1 (MQ=255) aTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcat < 1:720501/71‑1 (MQ=255) aTGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcat < 1:197432/71‑1 (MQ=255) tGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACAttt < 1:1751292/42‑1 (MQ=255) tGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACAttt < 1:818566/42‑1 (MQ=255) gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatca < 1:487634/70‑1 (MQ=255) gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatca < 1:2032456/70‑1 (MQ=255) gATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAg > 1:2126155/1‑71 (MQ=255) gATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAg > 1:187345/1‑71 (MQ=255) attattAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGaa < 1:3097386/59‑1 (MQ=255) ttattaGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGt > 1:3452272/1‑70 (MQ=255) ttattaGTCTTTGCATGAGCAAGCGAACTGATACATATCTCTTTGTTCTCATTCAGAAAATCtca < 1:3953464/65‑1 (MQ=255) | AAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGT > minE/2823811‑2823938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |