| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,827,357 | G→A | 52.0% | A298V (GCA→GTA) | dcuA ← | C4‑dicarboxylate antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,827,357 | 0 | G | A | 52.0% | ‑2.1 / 110.5 | 75 | A298V (GCA→GTA) | dcuA | C4‑dicarboxylate antiporter |
| Reads supporting (aligned to +/- strand): ref base G (18/18); new base A (14/25); total (32/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01 | |||||||||||
GTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGA > minE/2827288‑2827423 | gTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGc < 1:3940276/71‑1 (MQ=255) cGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGc > 1:3525425/1‑69 (MQ=255) cGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGc > 1:2561874/1‑69 (MQ=255) cAGAATGAACAGCCCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCagag > 1:49350/1‑71 (MQ=255) aGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGc < 1:936149/65‑1 (MQ=255) aGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGc < 1:3517165/65‑1 (MQ=255) aaTGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCa > 1:2013617/1‑71 (MQ=255) tGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATc < 1:3249699/71‑1 (MQ=255) aaCAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCAt > 1:1115424/1‑68 (MQ=255) aaCAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCgg > 1:2816025/1‑71 (MQ=255) cagaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCa > 1:477650/1‑71 (MQ=255) agaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCAt < 1:2382624/71‑1 (MQ=255) gacgagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCAt < 1:568435/70‑1 (MQ=255) gaccagacACCGCAGCGAAAGAAGCAACAGGGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATc < 1:2838293/71‑1 (MQ=255) gaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATc < 1:3505826/71‑1 (MQ=255) gaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCAt > 1:460566/1‑70 (MQ=255) gaccagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCAt > 1:465492/1‑70 (MQ=255) cagacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGt > 1:148141/1‑71 (MQ=255) gacACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGc < 1:3360491/71‑1 (MQ=255) acacCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGc < 1:1789858/55‑1 (MQ=255) acacCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCt < 1:1265864/71‑1 (MQ=255) cacCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCtt < 1:1263857/71‑1 (MQ=255) acCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCa > 1:817863/1‑55 (MQ=255) tcAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCATTACCAGAGc < 1:3978942/49‑1 (MQ=38) gCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCa > 1:27663/1‑52 (MQ=255) gCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCa > 1:1664361/1‑61 (MQ=255) cAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCg < 1:760327/71‑1 (MQ=255) gCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCtt < 1:1086509/64‑1 (MQ=255) cGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGtt > 1:2285654/1‑71 (MQ=255) gAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg < 1:1187818/71‑1 (MQ=255) gAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg > 1:326627/1‑71 (MQ=255) aaaGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg > 1:660755/1‑70 (MQ=255) aaaGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATc > 1:436301/1‑48 (MQ=255) aaaGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg > 1:2639429/1‑70 (MQ=255) aaaGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGc < 1:373301/71‑1 (MQ=255) aagaagCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCttt > 1:1996985/1‑61 (MQ=255) agaagCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCa > 1:3530644/1‑50 (MQ=255) agaagCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAg < 1:2740174/71‑1 (MQ=255) agaagCAACAGCGGTCAGCGCTGAAACGTTCAGTGCCAGAGCCATCGGCa > 1:3525875/1‑50 (MQ=255) gaagCAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGc < 1:1418006/71‑1 (MQ=255) gCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTg < 1:2547253/71‑1 (MQ=255) cAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTga < 1:744151/71‑1 (MQ=255) cAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGt < 1:1454339/61‑1 (MQ=255) cAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAg < 1:1754437/66‑1 (MQ=255) cAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAg < 1:3402266/66‑1 (MQ=255) cAACAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTga < 1:740465/71‑1 (MQ=255) aaCAGCGGTCAGCTGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTga < 1:834654/70‑1 (MQ=255) aCAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCAt < 1:1542013/44‑1 (MQ=255) aCAGCGGTCAGCGGTGAAACGTTCAGTACCAGAGCCATCGGCATCAGt < 1:1304057/48‑1 (MQ=255) cagcggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagag < 1:54505/71‑1 (MQ=255) cagcggtcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagag < 1:3355769/71‑1 (MQ=255) cagcggtcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagag < 1:1412708/71‑1 (MQ=255) cagcggtcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagag < 1:2322638/71‑1 (MQ=255) agcggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGt > 1:1778448/1‑46 (MQ=255) agcggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGt < 1:1303808/71‑1 (MQ=255) agcggtcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGagag < 1:1358983/70‑1 (MQ=255) gcggtcagcgttGAAACGTTCAGTACCAGAGCCATCGGCATCa > 1:1542326/1‑43 (MQ=38) gcggtcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTa > 1:1509770/1‑71 (MQ=255) ggtcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCttt > 1:467041/1‑48 (MQ=255) tcagcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGc < 1:4055964/49‑1 (MQ=255) tcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCtt < 1:3824400/45‑1 (MQ=255) tcagcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTa > 1:1813675/1‑67 (MQ=255) agcggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTga > 1:119673/1‑60 (MQ=255) gcggtGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTg < 1:1283334/45‑1 (MQ=255) ggtGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACagcagag < 1:3173733/70‑1 (MQ=255) tGAAACGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACagcagagcag < 1:3861806/71‑1 (MQ=255) gAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCa > 1:1617009/1‑48 (MQ=255) gAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCa > 1:3518380/1‑48 (MQ=255) aaaCGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTg > 1:3466354/1‑52 (MQ=255) aaaCGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACagcagagcag > 1:2531554/1‑69 (MQ=255) aCGTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCt < 1:3552552/49‑1 (MQ=255) gTTCAGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCt < 1:3064720/47‑1 (MQ=255) cAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCaatg > 1:1166505/1‑68 (MQ=255) aGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAaaga < 1:3419423/70‑1 (MQ=255) aGTACCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAaaga < 1:1404452/70‑1 (MQ=255) | GTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGA > minE/2827288‑2827423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |