| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,870,829 | G→A | 23.4% | intergenic (+72/‑108) | rnr → / → rlmB | exoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,870,829 | 0 | G | A | 23.4% | 185.9 / 54.7 | 94 | intergenic (+72/‑108) | rnr/rlmB | exoribonuclease R, RNase R/23S rRNA (Gm2251)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (30/42); new base A (12/10); total (42/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
AATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAA > minE/2870760‑2870896 | aaTACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:2423053/70‑1 (MQ=255)aaTACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:566109/70‑1 (MQ=255) aTACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa < 1:4114383/71‑1 (MQ=255) tACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc > 1:1041946/1‑71 (MQ=255) tACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAAc > 1:3206774/1‑71 (MQ=255) ccTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc < 1:1296359/71‑1 (MQ=255) ctctTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc > 1:708021/1‑70 (MQ=255) tttAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:3900017/61‑1 (MQ=255) tttAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:739766/69‑1 (MQ=255) ttAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc > 1:910373/1‑71 (MQ=255) ttAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc > 1:1140225/1‑71 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTAt < 1:1943384‑M2/23‑1 (MQ=255) gcgtaggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATc > 1:3671455‑M2/48‑71 (MQ=255) tAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:3770162/59‑1 (MQ=255) tAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:1184460/68‑1 (MQ=255) tAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc > 1:464967/1‑71 (MQ=255) tAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc > 1:454735/1‑71 (MQ=255) cgtaggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTaaa > 1:654835‑M2/47‑61 (MQ=255) gtaggcctgataagacgcggcaagcgtcgcatcaggcgttgatgtCGGATGCGGCGTAAACGCCTTATCCg < 1:1276829‑M2/26‑1 (MQ=255) aggcctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTaaa > 1:2150327‑M2/44‑58 (MQ=255) aagaagAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa > 1:446532/1‑58 (MQ=255) ggcctgataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGAcc < 1:3522756‑M2/29‑1 (MQ=255) gaagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:3589001/54‑1 (MQ=255) gaagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt > 1:2439585/1‑62 (MQ=255) gaagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc < 1:3105312/69‑1 (MQ=255) aagaGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg > 1:84484/1‑66 (MQ=255) ctgataagacgcggcaagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTa > 1:2123991‑M2/40‑70 (MQ=255) ataagacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTAcaa < 1:3291702‑M2/34‑2 (MQ=255) ggTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATaa < 1:888289/71‑1 (MQ=255) gTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAAt < 1:3427475/71‑1 (MQ=255) agacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAAt > 1:1847499‑M2/34‑70 (MQ=255) tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:3023671/45‑1 (MQ=255) tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:2285132/45‑1 (MQ=255) tAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCa > 1:3923109/1‑71 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATc > 1:2399343‑M2/33‑70 (MQ=255) gacgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATc < 1:3374261‑M2/38‑1 (MQ=255) acgcgttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCAc > 1:675455‑M2/32‑71 (MQ=255) aGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa > 1:3544240/1‑46 (MQ=255) aTTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:3381010/51‑1 (MQ=255) tGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGaa > 1:3700094/1‑42 (MQ=255) tGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGc < 1:869695/45‑1 (MQ=255) tGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCa > 1:2262234/1‑70 (MQ=255) tGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCa > 1:1983495/1‑70 (MQ=255) gttaagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGc > 1:2794429‑M2/28‑69 (MQ=255) cTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCaaa < 1:2552033/70‑1 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACgctaaat > 1:529918‑M2/25‑69 (MQ=255) aagcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACgctaaat > 1:1651814‑M2/25‑69 (MQ=255) aagcgtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATCCGACCTACAAAATCACgctaaat > 1:1748216‑M2/25‑69 (MQ=255) gACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAAtt > 1:3104871/1‑70 (MQ=255) gcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaa < 1:3022600‑M2/46‑2 (MQ=255) gcgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGctaaa < 1:2349645‑M2/46‑2 (MQ=255) aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAAtt < 1:2459684/69‑1 (MQ=255) aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTc < 1:780167/70‑1 (MQ=255) aCAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCa < 1:386602/71‑1 (MQ=255) cgtcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATc < 1:923694‑M2/38‑1 (MQ=255) gtcgcatcaggcattgatgtCGGATGCGGCGTAAACGCCTTATccgac > 1:405859‑M2/21‑46 (MQ=255) aaaaTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACATaa > 1:2977763/1‑56 (MQ=255) aaaaTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:1856755/42‑1 (MQ=38) aaaaTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATa > 1:2457055/1‑55 (MQ=40) aaaaTGCGCTTTGTTCATGCCGGAAGCGGCGTGAACGCCTTa < 1:1851950/42‑1 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAatcaggc < 1:429367‑M2/52‑4 (MQ=255) tcgcatcaggcgttgatgtCGGATGCGGCATAAACGCCTTATCCGACCTACAAAATCACGCTAAatcaggc < 1:688005‑M2/52‑4 (MQ=255) aaaTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAata < 1:3025956/71‑1 (MQ=255) aaTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCa > 1:3154997/1‑57 (MQ=255) aaTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTc < 1:918536/66‑1 (MQ=255) tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAata < 1:3970162/68‑1 (MQ=255) cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa < 1:1643017/71‑1 (MQ=255) cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa < 1:3756211/71‑1 (MQ=255) cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa < 1:714569/71‑1 (MQ=255) cTTTGTTCATGCCGGATGCGGCATGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTg > 1:394698/1‑69 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAata < 1:3280597/63‑1 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa < 1:3334544/70‑1 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa < 1:3373258/70‑1 (MQ=255) ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatatat > 1:1006746/1‑65 (MQ=255) tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa > 1:3084404/1‑68 (MQ=255) tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa > 1:138408/1‑68 (MQ=255) gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATAtt < 1:2707301/64‑1 (MQ=255) ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa > 1:3830489/1‑66 (MQ=255) ttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAgaga < 1:371141/70‑1 (MQ=255) ctCATGCCGGATGCGGCATGAGTACCATATCCTTCCT‑GAAAATCGCGCAAATTCTATATATTGCAGAGAt > 1:2779281/2‑70 (MQ=2) tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATg < 1:789907/71‑1 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATg < 1:1598197/71‑1 (MQ=255) gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATg > 1:1312621/1‑70 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa > 1:1453972/1‑60 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa > 1:3671550/1‑60 (MQ=255) cGGATGCGGCGTGAACGCCTGATCCGGCCTACATAATCACGCAAATTCaa < 1:3465948/50‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCa < 1:61650/47‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGc > 1:3537357/1‑71 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGc < 1:2253416/71‑1 (MQ=255) gATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGc > 1:3081884/1‑71 (MQ=255) tGCCGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAata < 1:775932/48‑1 (MQ=255) ggcgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatat < 1:333423/46‑1 (MQ=255) gcgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCaa < 1:1412442/42‑1 (MQ=255) cgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATaa < 1:3122007/71‑1 (MQ=255) cgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATaa < 1:920698/71‑1 (MQ=255) cgTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGCCCGGATaa < 1:3544543/71‑1 (MQ=255) tGAACGCCTTATCCGGCCTACATAATCACGCAAATTc < 1:2420706/37‑1 (MQ=255) tGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAatat < 1:459681/42‑1 (MQ=255) tGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCa < 1:343781/48‑1 (MQ=255) tGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAgaga < 1:2491526/52‑1 (MQ=255) | AATACCCTCTTTAAAAGAAGAGGGTTAGATTGCTGACAAAATGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAATCACGCAAATTCAATATATTGCAGAGATCATGTAGGCCGGATAA > minE/2870760‑2870896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |