| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,877,709 | T→A | 25.8% | intergenic (+112/‑197) | fklB → / → cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,877,709 | 0 | T | A | 25.8% | 109.8 / 40.5 | 62 | intergenic (+112/‑197) | fklB/cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (22/24); new base A (10/6); total (32/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
CAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTA > minE/2877641‑2877778 | cAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTAttttt > 1:1598629/1‑71 (MQ=255) cAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTtgt > 1:517612/1‑70 (MQ=255) aaaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCt > 1:3710726/1‑71 (MQ=255) aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCt < 1:1791081/70‑1 (MQ=255) aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCt < 1:218082/70‑1 (MQ=255) cccTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGtt > 1:1603885/1‑71 (MQ=255) ttCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTc > 1:3050059/1‑70 (MQ=255) cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTcgc < 1:1212365/70‑1 (MQ=255) aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGt < 1:2225178/71‑1 (MQ=255) aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGt > 1:3970668/1‑71 (MQ=255) tttGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTa > 1:2045108/1‑71 (MQ=255) tGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTAt > 1:1449040/1‑70 (MQ=255) ccAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTc < 1:1961778/61‑1 (MQ=255) cAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCAcc > 1:172171/1‑71 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:3637281/71‑1 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1682942/71‑1 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTCGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1738159/71‑1 (MQ=255) ggCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1022774/70‑1 (MQ=255) cgcTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:3080940/71‑1 (MQ=255) cgcTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:2717779/71‑1 (MQ=255) cgcTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:3736481/71‑1 (MQ=255) cTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:3628240/56‑1 (MQ=255) cTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGa < 1:2242853/71‑1 (MQ=255) cTATTTGGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATg > 1:1863/1‑70 (MQ=255) tATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGt < 1:1401159/54‑1 (MQ=255) aTTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGata > 1:3215279/1‑66 (MQ=255) aTTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa > 1:724179/1‑64 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGtt < 1:3675735/46‑1 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATc < 1:1544117/56‑1 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:3231060/1‑70 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:1016952/1‑70 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact < 1:1090014/70‑1 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:239918/1‑70 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:3603268/1‑70 (MQ=255) ttGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTc < 1:2111259/41‑1 (MQ=255) tGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACtt > 1:3802192/1‑69 (MQ=255) gCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTAt > 1:2010108/1‑46 (MQ=255) gCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTg > 1:485373/1‑35 (MQ=255) gCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTg > 1:1464709/1‑35 (MQ=255) aaGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATc < 1:2346269/44‑1 (MQ=255) gACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGa > 1:1470635/1‑49 (MQ=255) cccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCAc > 1:2083138/1‑41 (MQ=255) cccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaaaa > 1:3616581/1‑71 (MQ=255) ccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGt < 1:2057776/43‑1 (MQ=255) cGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt < 1:2780623/71‑1 (MQ=255) cGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGATATCACCGTGATATGACACTCACTTTAAACATAAAAtt < 1:3394056/71‑1 (MQ=255) cGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt < 1:469065/71‑1 (MQ=255) cGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt < 1:3383899/71‑1 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCAGCGTGATATGACACTCACTTTAAACATAAAATTa < 1:3792592/71‑1 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt > 1:3131546/1‑70 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTa < 1:1236299/71‑1 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTa < 1:3141727/71‑1 (MQ=255) gTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt > 1:1260076/1‑70 (MQ=255) gTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAt > 1:2129382/1‑69 (MQ=255) tAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa > 1:2699043/1‑71 (MQ=255) aaGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa > 1:589204/1‑70 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGac > 1:1473974/1‑46 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:96367/1‑71 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:1991821/1‑71 (MQ=255) gtgtATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACttt > 1:2870420/1‑52 (MQ=255) gtgtATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaaaa < 1:292139/62‑1 (MQ=255) tgtATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATCACACTCACTTTAAACATAAAATTAACATTAg < 1:2445451/71‑1 (MQ=255) tATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt > 1:680340/1‑66 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactcac > 1:218358/1‑42 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:1410271/1‑39 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:1298536/1‑39 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATc > 1:2727979/1‑68 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTa > 1:2846874/1‑70 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTa > 1:2364418/1‑70 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTa > 1:1280091/1‑70 (MQ=255) | CAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTA > minE/2877641‑2877778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |