Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,891,414 C→A 23.3% P23P (CCC→CCA ytfQ → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,891,4140CA23.3% 156.7 / 25.4 60P23P (CCC→CCAytfQpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (20/26);  new base A (8/6);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAA  >  minE/2891345‑2891483
                                                                     |                                                                     
tATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCt                                                                      >  1:3093084/1‑71 (MQ=255)
tATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCt                                                                      >  1:1833109/1‑71 (MQ=255)
  tgtgGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCtt                                                                     <  1:4095140/70‑1 (MQ=255)
    ttgAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTaa                                                                   >  1:3093909/3‑70 (MQ=255)
     ggAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCACCATTAAc                                                                  >  1:3568149/1‑70 (MQ=255)
      gAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCtt                                                                     <  1:2578522/66‑1 (MQ=255)
      gAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCg                                                                >  1:1881119/1‑71 (MQ=255)
       aaaCGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGt                                                               <  1:2681617/71‑1 (MQ=255)
         aCGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTg                                                             <  1:2560067/71‑1 (MQ=255)
             ttacttaTAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTgg                                                            <  1:1055019/68‑1 (MQ=255)
               acttaTAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGAt                                                          >  1:1871649/1‑68 (MQ=255)
                   ataGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGtt                                                              >  1:4131934/1‑60 (MQ=255)
                    taGTCTCTTCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCg                                                     <  1:3652129/68‑1 (MQ=255)
                         tctGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcg                                              >  1:1445969/1‑70 (MQ=255)
                         tctGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcg                                              >  1:2048302/1‑70 (MQ=255)
                         tctGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGtcg                                              >  1:1799777/1‑70 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCtt                                                                     >  1:1957575/1‑45 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAg                                                  >  1:1919304/1‑64 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcgga                                            >  1:3993418/1‑70 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcgga                                            >  1:3677317/1‑70 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcgg                                             <  1:779304/69‑1 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAAACGTTGGATTTTCGCAGGtcgg                                             <  1:3652250/69‑1 (MQ=255)
                           tGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTAGATTTTCGCAGGtcgg                                             <  1:1579903/69‑1 (MQ=255)
                             cAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGtcggat                                           >  1:3187088/1‑69 (MQ=255)
                               gTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcggatcgga                                       <  1:2054071/71‑1 (MQ=255)
                                  tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAAt                                     >  1:77200/1‑70 (MQ=255)
                                  tcGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAAt                                     >  1:577478/1‑70 (MQ=255)
                                  tcGGCAGCCATGTCGTCTATGGCATTGGCCGCTCCCTTAACCGTTg                                                             >  1:210372/1‑46 (MQ=255)
                                        gCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCg                                                     <  1:4116345/48‑1 (MQ=255)
                                         ccATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTgg                             <  1:2906447/71‑1 (MQ=255)
                                            tGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGAtt                                                         <  1:2159242/40‑1 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGAttt                                                        <  1:216925/39‑1 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAg                                                  >  1:3905089/1‑45 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcgg                                             <  1:1092101/50‑1 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCa                                   >  1:3177618/1‑60 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTg                         >  1:3468479/1‑70 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGAtttt                                                       >  1:3226671/1‑40 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGAttt                                                        <  1:894106/39‑1 (MQ=255)
                                              tcgtcTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGc                        >  1:1984812/1‑71 (MQ=255)
                                                gtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCg                      >  1:3339448/1‑71 (MQ=255)
                                                 tcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcggatcgga                                       <  1:700587/53‑1 (MQ=255)
                                                     tGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                  <  1:4079544/70‑1 (MQ=255)
                                                     tGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGa                  <  1:933882/70‑1 (MQ=255)
                                                      ggCGTTGGCCGCTCCCTTAACCGTTGAATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAg                   <  1:3396226/68‑1 (MQ=255)
                                                       gCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGaaa                <  1:2960024/70‑1 (MQ=255)
                                                       gCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGaaa                <  1:1785857/70‑1 (MQ=255)
                                                       gCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGaaa                <  1:2900838/70‑1 (MQ=255)
                                                       gCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAAc               >  1:3179265/1‑71 (MQ=255)
                                                       gCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAAc               >  1:4101825/1‑71 (MQ=255)
                                                        cGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAAcc              <  1:608461/71‑1 (MQ=255)
                                                        cGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAAc               >  1:1619006/1‑70 (MQ=255)
                                                          ttGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTgg                             <  1:2581691/54‑1 (MQ=255)
                                                          ttGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCaa            <  1:2685258/71‑1 (MQ=255)
                                                            ggCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAgg                                 >  1:124320/1‑48 (MQ=255)
                                                             gCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAAtgt         <  1:2800584/71‑1 (MQ=255)
                                                              ccGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGc                                <  1:3309025/47‑1 (MQ=255)
                                                              ccGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGc                                <  1:3661716/47‑1 (MQ=255)
                                                              ccGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGc                                <  1:2976447/47‑1 (MQ=255)
                                                                 cTCCCTTAACCGTTGTATTTTCGCAGGTCGGATCGGAATCAGGCTgg                             <  1:997683/47‑1 (MQ=255)
                                                                 cTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCa             <  1:342514/63‑1 (MQ=255)
                                                                   cccTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAAt           >  1:2165302/1‑63 (MQ=255)
                                                                    ccTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGaaa  <  1:2226918/71‑1 (MQ=255)
                                                                    ccTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGaa   <  1:1948653/70‑1 (MQ=255)
                                                                     cTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGaa   >  1:2308311/1‑69 (MQ=255)
                                                                     cTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGaa   >  1:2586005/1‑69 (MQ=255)
                                                                     cTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGaa   >  1:2140983/1‑69 (MQ=255)
                                                                     |                                                                     
TATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAA  >  minE/2891345‑2891483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: