| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,673 | G→A | 9.6% | L400L (CTC→CTT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,673 | 0 | G | A | 9.6% | 228.5 / 10.8 | 73 | L400L (CTC→CTT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (35/30); new base A (2/5); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01 | |||||||||||
TCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCC > minE/311605‑311740 | tcttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:286891/1‑71 (MQ=255)tcttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:2272152/1‑71 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:2593503/1‑70 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:2022659/70‑1 (MQ=255) ccGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGc < 1:158362/68‑1 (MQ=255) cGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCAcc < 1:2438285/70‑1 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCg < 1:2766905/59‑1 (MQ=255) aaCACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATc > 1:495542/1‑67 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGa > 1:999226/1‑58 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa > 1:2754868/1‑71 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa > 1:4083210/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa < 1:277212/71‑1 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa < 1:1344686/71‑1 (MQ=255) cGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCg > 1:1691291/1‑54 (MQ=255) cGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg < 1:1523054/66‑1 (MQ=255) gCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAtt > 1:1042670/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCa > 1:2775837/1‑55 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:249664/1‑71 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCa < 1:3409455/54‑1 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc < 1:1421849/71‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCTAGCACCATCCCGAACATTGTTAGc < 1:866525/69‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc < 1:2887343/69‑1 (MQ=255) tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGtt > 1:4134395/1‑71 (MQ=255) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga < 1:2056628/70‑3 (MQ=9) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa < 1:1212382/60‑1 (MQ=255) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGttt < 1:1844710/68‑1 (MQ=255) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa < 1:1766885/69‑1 (MQ=255) acaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:3972220/71‑1 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc > 1:2141354/1‑68 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCACGAACATTGTTAGCGTGttt > 1:1351879/1‑65 (MQ=255) aaCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga < 1:2470879/71‑1 (MQ=255) aCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag > 1:988806/1‑69 (MQ=255) aCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag > 1:2723166/1‑69 (MQ=255) gATGGCGTCATCCACCATCAGGCCGATGGCGAGCTCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAg < 1:4105831/70‑1 (MQ=255) gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa < 1:2358204/67‑1 (MQ=9) gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:3728942/4‑70 (MQ=9) tCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGtt < 1:1742676/45‑1 (MQ=12) cATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa < 1:2515136/54‑1 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa > 1:3510059/1‑44 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa > 1:304189/1‑44 (MQ=255) ccaccaACAGGCCGATGGCGAGCACCATCCCGAACa < 1:1693656/36‑1 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg < 1:78444/46‑1 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag > 1:1643518/1‑55 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc > 1:1610436/1‑69 (MQ=255) caccaACAGGCCGATGGCAAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc > 1:2142968/1‑69 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg > 1:3044013/1‑71 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAg > 1:389126/1‑38 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:1331522/1‑71 (MQ=255) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag < 1:2436159/50‑1 (MQ=255) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:176189/1‑71 (MQ=255) cAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg > 1:1848640/1‑40 (MQ=255) cAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGc > 1:420902/1‑53 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag < 1:779333/48‑1 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa < 1:1791344/67‑1 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc > 1:190568/1‑70 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc > 1:3299922/1‑70 (MQ=255) gcccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGtt < 1:648351/38‑1 (MQ=21) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:93436/1‑56 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:3879823/1‑56 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2469561/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1182417/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1963441/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2540081/71‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaaa < 1:3136104/53‑1 (MQ=12) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:2602027/69‑1 (MQ=255) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa > 1:2581942/1‑69 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa > 1:2716595/1‑68 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:2415528/71‑1 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:774543/1‑71 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:934981/71‑1 (MQ=255) gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:2622223/60‑1 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt < 1:2688022/71‑1 (MQ=255) ggggAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:1622186/4‑71 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:3103408/1‑67 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:2852074/1‑67 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:1548159/1‑71 (MQ=255) | TCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCC > minE/311605‑311740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |