| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,906,377 | G→T | 6.5% | R396R (CGC→CGA) | treB ← | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,906,377 | 0 | G | T | 6.5% | 460.1 / 8.3 | 135 | R396R (CGC→CGA) | treB | fused trehalose(maltose)‑specific enzyme IIBC component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (57/69); new base T (5/4); total (62/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 138 total aligned reads displayed.
CCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGG > minE/2906307‑2906445 | cATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGc < 1:4062955/71‑1 (MQ=255) aTAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGc > 1:2073305/1‑70 (MQ=255) tAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGa < 1:1833382/71‑1 (MQ=255) tAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGa < 1:4035108/71‑1 (MQ=255) aCGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGata > 1:3613535/1‑71 (MQ=255) aCGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGata > 1:2127205/1‑71 (MQ=255) gCCGTTCAGGTCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGata > 1:1643843/1‑69 (MQ=255) gCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAtt > 1:367556/1‑71 (MQ=255) cGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAttt > 1:755013/1‑70 (MQ=255) cGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAttt > 1:2210452/1‑70 (MQ=255) cGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAttt < 1:2856599/70‑1 (MQ=255) ttCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGat > 1:2335898/1‑64 (MQ=255) ttCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAttt < 1:2164481/68‑1 (MQ=255) tCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAATCGATATTTCAgg > 1:3968000/1‑71 (MQ=255) cAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAATCGATATTTCAGGt > 1:1384134/1‑71 (MQ=255) cAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt > 1:1510077/1‑71 (MQ=255) cAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt > 1:2779118/1‑71 (MQ=255) aGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt > 1:2611987/1‑71 (MQ=255) gCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGa > 1:3367068/1‑58 (MQ=255) gCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGa < 1:123861/71‑1 (MQ=255) ccGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGata > 1:447889/1‑59 (MQ=255) gCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCa < 1:3966348/62‑1 (MQ=255) cATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGcc > 1:2526248/1‑71 (MQ=255) cATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGcc < 1:3489080/71‑1 (MQ=255) aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGat < 1:593391/54‑1 (MQ=255) aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCg > 1:674973/1‑71 (MQ=255) aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCg < 1:3083623/71‑1 (MQ=255) aTAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCg > 1:360263/1‑71 (MQ=255) tAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAATCGATATTTCa < 1:1654019/59‑1 (MQ=255) tAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATAttt < 1:2459873/57‑1 (MQ=255) tAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCg > 1:1415784/1‑70 (MQ=255) gCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa > 1:3216564/1‑70 (MQ=255) gCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa < 1:1412273/70‑1 (MQ=255) gCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa < 1:3618352/70‑1 (MQ=255) gCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa > 1:921401/1‑70 (MQ=255) gCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa < 1:728127/70‑1 (MQ=255) gCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa > 1:2060170/1‑70 (MQ=255) aaTCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATg > 1:48624/1‑63 (MQ=255) aaTCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACAt < 1:1153302/71‑1 (MQ=255) aTCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGatat > 1:716299/1‑50 (MQ=255) aTCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGatat > 1:3992389/1‑50 (MQ=255) tCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAATCGa < 1:1073390/46‑1 (MQ=255) tCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt < 1:2856729/57‑1 (MQ=255) tCCTGCCAGACCAGAACCAATCATCGCGCAAAGCATCGGGAATCGa < 1:3224508/46‑1 (MQ=255) ccTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGcc > 1:2719176/1‑62 (MQ=255) cTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCg > 1:2178675/1‑62 (MQ=255) cTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTg > 1:1966235/1‑69 (MQ=255) cTGCCAGACCAGAACCAATCACCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTg > 1:1606235/1‑69 (MQ=255) gCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt > 1:1732372/1‑52 (MQ=255) ccagacgagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGc < 1:1378676/71‑1 (MQ=255) ccagaccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGc < 1:1673173/71‑1 (MQ=255) cagaccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCt < 1:1731817/71‑1 (MQ=255) agaccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:1879/71‑1 (MQ=255) gaccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt < 1:2792984/49‑1 (MQ=255) accagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCa < 1:179904/44‑1 (MQ=255) ccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt < 1:3986205/47‑1 (MQ=255) ccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt < 1:2658809/71‑1 (MQ=255) ccagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt > 1:740332/1‑71 (MQ=255) cagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGtt > 1:3903181/1‑46 (MQ=255) cagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa < 1:2928472/55‑1 (MQ=255) cagaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt > 1:749893/1‑70 (MQ=255) agaACCAATCATCGCGCACAGCATCGGGAAGCGATAtt > 1:1306891/1‑38 (MQ=255) agaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAgg > 1:2280640/1‑43 (MQ=255) gaACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATg > 1:3863660/1‑48 (MQ=255) aaCCAATCATCGCGCACAGCATCGGGAAGCGTTAttt < 1:949497/37‑1 (MQ=255) aaCCAATCATCGCGCACAGCATCGGGAAGCGATAttt < 1:2961678/37‑1 (MQ=255) aaCCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa < 1:335184/52‑1 (MQ=255) aCCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGAccc < 1:2094660/71‑1 (MQ=255) ccAATCATCGCGCACAGCATCGGGAATCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGAccc > 1:1087645/1‑70 (MQ=255) ccAATCATCGCGCACAGCATCGGGAATCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGAccc > 1:3661995/1‑70 (MQ=255) ccAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGAccc > 1:2809551/1‑70 (MQ=255) aaTCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAg > 1:2572666/1‑56 (MQ=255) atcatcGCGCACAGCATCGGGAATCGATATTTCAGGTTGa > 1:2128791/1‑40 (MQ=255) tcatcGCGCACAGCATCGGGAAGCGATATTTCAGGtt < 1:2770766/37‑1 (MQ=255) tcatcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCt < 1:3285208/57‑1 (MQ=255) tcatcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc < 1:1072350/71‑1 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGcc < 1:760296/70‑1 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:3182537/1‑69 (MQ=255) atcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGc > 1:486933/1‑69 (MQ=255) tcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCa < 1:430414/56‑1 (MQ=255) tcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt < 1:3995028/58‑1 (MQ=255) tcGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt < 1:2239045/58‑1 (MQ=255) cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1620756/71‑1 (MQ=255) cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:2283516/71‑1 (MQ=255) cacaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:1929155/71‑1 (MQ=255) acaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTc < 1:1666166/49‑1 (MQ=255) acaGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGga < 1:3153850/70‑1 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGt < 1:2392606/51‑1 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCg > 1:2274904/1‑67 (MQ=255) caGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGgcga < 1:1781932/71‑1 (MQ=255) cATCGGGAAGCGATATTTCAGGTTGATGCCGTACAtt > 1:1370179/1‑37 (MQ=255) aTCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt < 1:762802/68‑1 (MQ=255) tCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCt < 1:1512447/42‑1 (MQ=255) tCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCaa < 1:3450022/54‑1 (MQ=255) tCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGAt > 1:2200779/1‑67 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAg > 1:3440855/1‑43 (MQ=255) gggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCgg > 1:3725199/1‑70 (MQ=255) ggAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggc < 1:2322545/70‑1 (MQ=255) aaGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggcagg < 1:262679/71‑1 (MQ=255) gCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggcagg < 1:4098737/69‑1 (MQ=255) | CCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGG > minE/2906307‑2906445 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |