| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,021 | A→G | 5.2% | G225G (GGT→GGC) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,021 | 0 | A | G | 5.2% | 266.2 / 5.5 | 96 | G225G (GGT→GGC) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (45/45); new base G (3/2); total (49/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01 | |||||||||||
CAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCGG > minE/2918951‑2919090 | caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCa > 1:1749111/1‑71 (MQ=255)caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCa > 1:788911/1‑71 (MQ=255) aatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAc < 1:1655502/71‑1 (MQ=255) atCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAcc > 1:1118060/1‑71 (MQ=255) tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCt > 1:1191696/1‑71 (MQ=255) ccGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATAAAGTCCGCGccct > 1:3953663/1‑69 (MQ=255) cGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTc < 1:3473949/71‑1 (MQ=255) ttCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCaa < 1:491313/71‑1 (MQ=255) ttCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCaa < 1:2543313/71‑1 (MQ=255) ccATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCt > 1:4072501/1‑71 (MQ=255) ccATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCt > 1:4035780/1‑71 (MQ=255) cATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCtt > 1:207836/1‑71 (MQ=255) cATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCtt > 1:3879067/1‑71 (MQ=255) aTTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTc > 1:1376554/1‑71 (MQ=255) tttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCa < 1:2886486/61‑1 (MQ=255) ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgc > 1:55714/1‑71 (MQ=255) ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgc > 1:1179593/1‑71 (MQ=255) ttCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgc > 1:3351464/1‑71 (MQ=255) ttCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATAAAGTCCGCGCCCTTAAcg < 1:3108798/63‑2 (MQ=255) tctcTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgc < 1:389013/70‑1 (MQ=255) tctcTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATAAAGTCCGCGccct > 1:1783061/1‑55 (MQ=255) ctctTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgcg < 1:3346098/70‑1 (MQ=255) ctctTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgcg < 1:2465857/70‑1 (MQ=255) tctTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa > 1:912239/1‑70 (MQ=255) ttttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa > 1:1207318/1‑68 (MQ=255) ttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCt > 1:1708280/1‑71 (MQ=255) ttGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCt > 1:906231/1‑71 (MQ=255) tGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCtt > 1:2821745/1‑71 (MQ=255) cccccATCGACACCCACACATCGGTATAGATAAAGTCAGCAcc > 1:2565414/1‑43 (MQ=255) cccccATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagt > 1:187321/1‑70 (MQ=255) cccccATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagt > 1:232742/1‑70 (MQ=255) ccATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTcc > 1:892692/1‑49 (MQ=255) aTCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt < 1:3874861/71‑1 (MQ=255) cGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa > 1:1279946/1‑71 (MQ=255) cGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa > 1:1888978/1‑71 (MQ=255) gACACCGACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa > 1:533363/1‑70 (MQ=255) gACACCCACACATCGGTATAGATATAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa < 1:2831453/70‑1 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa > 1:2628101/1‑52 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGAc < 1:950714/53‑1 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGAc < 1:1431769/53‑1 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCg > 1:1866518/1‑67 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa > 1:567329/1‑70 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa > 1:1843052/1‑70 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa < 1:2329144/70‑1 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa < 1:25592/70‑1 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa > 1:1419252/1‑70 (MQ=255) gACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAat < 1:3871625/71‑1 (MQ=255) gACACACACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa > 1:4143229/1‑52 (MQ=255) cacCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCTACATCTTCAGTCAGCGTAat > 1:1353509/1‑69 (MQ=255) cacCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa > 1:2165919/1‑50 (MQ=255) cacCCACACATCGGTATAGATAAAGTCAGCACATTc > 1:525436/1‑36 (MQ=255) acCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATAtt < 1:3445834/71‑1 (MQ=255) acCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATAtt < 1:2641345/71‑1 (MQ=255) cccACACATCGGTATAGATAAAGTCAGCACCTTCa < 1:819463/35‑1 (MQ=255) cccACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCt < 1:1630634/42‑1 (MQ=255) cccACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGa < 1:2442358/48‑1 (MQ=255) cacacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTc > 1:1366151/1‑42 (MQ=255) cacacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGAc > 1:1417169/1‑47 (MQ=255) cacacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt < 1:3474849/62‑1 (MQ=255) cacacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTcc > 1:814860/1‑70 (MQ=255) acacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTa < 1:3881004/62‑1 (MQ=255) cacgTCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTAATTTTcc > 1:2494269/1‑68 (MQ=255) cacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtc < 1:625418/51‑1 (MQ=255) cacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCccac > 1:2873968/1‑71 (MQ=255) cacaTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCccac > 1:2303700/1‑71 (MQ=255) acaTCGGTATAGATAAAGTCAGCCCCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCcca > 1:3146804/1‑69 (MQ=255) acaTCGGTATAGATAAAGTCAGCACCTTCAACTccc < 1:277066/36‑1 (MQ=255) caTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAata > 1:1440053/1‑62 (MQ=255) caTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCccacca < 1:3201278/71‑1 (MQ=255) gtCGGTATAGATAAAGTCCGCGCCCTTAACGCCTGCCGCCACGTCTTCCGTCAGAGTAATTTTCccgccg < 1:2594638/69‑2 (MQ=255) aTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCAt < 1:117986/71‑1 (MQ=255) aTCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCAt < 1:2323555/71‑1 (MQ=255) tCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTcgcg < 1:435819/39‑1 (MQ=255) cGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagtc > 1:1639524/1‑50 (MQ=255) ggTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCAtttt < 1:3512839/71‑1 (MQ=255) tataGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCg < 1:3975790/51‑1 (MQ=255) ataGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagtca < 1:2388900/47‑1 (MQ=255) ataGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCt > 1:3518149/1‑71 (MQ=255) gATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTcc > 1:1268009/1‑56 (MQ=255) gATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGACAGCGTAATATTCCCACCATTTTGCtgt < 1:286310/70‑1 (MQ=255) aTAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa < 1:1023977/49‑1 (MQ=255) aTAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa < 1:851402/49‑1 (MQ=255) tAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTtcagt > 1:1902287/1‑40 (MQ=255) tAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGcc < 1:3676623/71‑1 (MQ=255) aaaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGc > 1:1057399/1‑69 (MQ=255) aaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGt < 1:2252295/44‑1 (MQ=255) aaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCa < 1:601629/70‑1 (MQ=255) aaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCa < 1:691638/70‑1 (MQ=255) aaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAg > 1:4007706/1‑71 (MQ=255) aaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAg > 1:1264572/1‑71 (MQ=255) aaGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAg < 1:1350344/71‑1 (MQ=255) tCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTaa < 1:3160720/43‑1 (MQ=255) tCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGcc < 1:1180054/66‑1 (MQ=255) cAGCACCTTCAACTCCCTTCGCGACATGTTCAGTCAGCGTAATATTCCCACCATTTTGCtg > 1:481170/1‑61 (MQ=255) cACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCgg < 1:540574/71‑1 (MQ=255) cACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCgg < 1:1036299/71‑1 (MQ=255) cACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCgg < 1:3839254/71‑1 (MQ=255) cACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTCCTGTGCCAGGGCGCgg < 1:3144181/71‑1 (MQ=255) | CAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTCAACTCCCTTCGCGACATCTTCAGTCAGCGTAATATTCCCACCATTTTGCTGTGCCAGGGCGCGG > minE/2918951‑2919090 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |