| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,159 | A→C | 6.5% | L179L (CTT→CTG) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,159 | 0 | A | C | 6.5% | 162.3 / 6.5 | 62 | L179L (CTT→CTG) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (24/34); new base C (2/2); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAT > minE/2919089‑2919227 | ggCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTa < 1:1871865/71‑1 (MQ=255) gCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTaa < 1:1956572/71‑1 (MQ=255) ccGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCGAATCCAGACCGGTAAGcgcc > 1:3204820/1‑71 (MQ=255) ccGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgcc > 1:3102944/1‑71 (MQ=255) ccGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgcc > 1:2933541/1‑71 (MQ=255) ccGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgcc > 1:1547302/1‑71 (MQ=255) gTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgccg > 1:3976433/1‑70 (MQ=255) tAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgccgc < 1:1639513/70‑1 (MQ=255) ccAGCGCAGCTTCCGGCCAGCACGCTTGTTGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCt > 1:1133181/1‑71 (MQ=255) ccAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCt > 1:3464485/1‑71 (MQ=255) cAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCtt > 1:2574056/1‑71 (MQ=255) cAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCtt > 1:2202388/1‑71 (MQ=255) aGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTc < 1:415287/71‑1 (MQ=255) aGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTc < 1:2189107/71‑1 (MQ=255) cgcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTc > 1:871257/1‑69 (MQ=255) gcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGACGCAGCTTCGAg > 1:1889231/1‑71 (MQ=255) cAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTc > 1:2031368/1‑67 (MQ=255) cAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGc < 1:3771981/71‑1 (MQ=255) aGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGc < 1:118685/70‑1 (MQ=255) tcTTCCGGCCAGCAGGCTTTCGGGGCCAACAGGCGCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCAt > 1:800963/2‑71 (MQ=255) cTTCCGGCCAGCAGGCTTTCGGGGCCAACAGGCGCAGATCCAGCCCGGTCAGcgccgccgc > 1:1686392/1‑61 (MQ=255) cTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTc > 1:396156/1‑64 (MQ=255) tCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCtt > 1:2828900/1‑61 (MQ=255) tCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg > 1:1909483/1‑70 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGa < 1:3970053/70‑1 (MQ=255) ggCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAAtt < 1:2481428/71‑1 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:616015/71‑1 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt < 1:2825293/71‑1 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt < 1:1724167/71‑1 (MQ=255) ttGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:586403/56‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:1649156/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:672418/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATgttgtt < 1:3894669/70‑1 (MQ=255) tgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAc < 1:3365917/71‑1 (MQ=255) gcgcGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcgc > 1:4046496/1‑71 (MQ=255) gACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGt < 1:1252158/71‑1 (MQ=255) ccAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCa < 1:865859/71‑1 (MQ=255) acAGGCGCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATcg < 1:1698637/70‑2 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGa > 1:740496/1‑38 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:283685/44‑1 (MQ=255) cAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:3397154/44‑1 (MQ=255) aGACGCAAATCCAGACCGGTAAGCGCCGCATCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAcc > 1:636375/1‑71 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:2070343/71‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:3903685/71‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGa < 1:2652221/40‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGa < 1:278062/40‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTg > 1:2229634/1‑44 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTc < 1:2806903/64‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCACCTTCGAGCATCGAATTGCCCATGTTGTTAc > 1:3790474/1‑57 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:2841360/38‑1 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGTGCGTCACCTGCa > 1:3907997/1‑70 (MQ=255) cAAATCCAGACCGGTAAGAGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:1794130/1‑70 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:3457089/1‑39 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa < 1:3379729/71‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcg < 1:3282971/55‑1 (MQ=255) aTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:1036567/42‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1816826/66‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1135382/66‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:2544179/66‑1 (MQ=255) ccAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAt < 1:968806/43‑1 (MQ=255) cAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGAcc < 1:2526445/70‑1 (MQ=255) cGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTc < 1:714916/71‑1 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt > 1:2721947/1‑71 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt < 1:3430471/71‑1 (MQ=255) | GGCATTCCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAT > minE/2919089‑2919227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |