| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,183 | A→G | 10.8% | N171N (AAT→AAC) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,183 | 0 | A | G | 10.8% | 202.4 / 14.6 | 74 | N171N (AAT→AAC) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (27/39); new base G (5/3); total (32/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAA > minE/2919115‑2919251 | ggCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAAtt < 1:2481428/71‑1 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:616015/71‑1 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt < 1:2825293/71‑1 (MQ=255) cGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgt < 1:1724167/71‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:672418/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:1649156/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATgttgtt < 1:3894669/70‑1 (MQ=255) tgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAc < 1:3365917/71‑1 (MQ=255) gcgcGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcgc > 1:4046496/1‑71 (MQ=255) gACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGt < 1:1252158/71‑1 (MQ=255) ccAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCa < 1:865859/71‑1 (MQ=255) acAGGCGCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATcg < 1:1698637/70‑2 (MQ=255) aGACGCAAATCCAGACCGGTAAGCGCCGCATCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAcc > 1:636375/1‑71 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:3903685/71‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:2070343/71‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTg > 1:2229634/1‑44 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTc < 1:2806903/64‑1 (MQ=255) gCAAATCCAGACCGGTAAGCGCCGCACCTTCGAGCATCGAATTGCCCATGTTGTTAc > 1:3790474/1‑57 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGTGCGTCACCTGCa > 1:3907997/1‑70 (MQ=255) cAAATCCAGACCGGTAAGAGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:1794130/1‑70 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:3457089/1‑39 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa < 1:3379729/71‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcg < 1:3282971/55‑1 (MQ=255) aTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:1036567/42‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1816826/66‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:2544179/66‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1135382/66‑1 (MQ=255) ccAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAt < 1:968806/43‑1 (MQ=255) cAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGAcc < 1:2526445/70‑1 (MQ=255) cGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTc < 1:714916/71‑1 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt < 1:3430471/71‑1 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt > 1:2721947/1‑71 (MQ=255) tcaGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCAtc > 1:3373137/3‑70 (MQ=255) aGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcgcgc > 1:2090193/1‑44 (MQ=255) aGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc > 1:2368877/1‑71 (MQ=255) aGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc > 1:73158/1‑71 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCa < 1:1950686/60‑1 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCa < 1:1818742/60‑1 (MQ=255) cgccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:3649841/1‑63 (MQ=255) ccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGtt > 1:929025/1‑70 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAcc < 1:3324485/45‑1 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa < 1:2055691/71‑1 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa < 1:1296743/71‑1 (MQ=255) cAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:632986/1‑47 (MQ=255) cAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:1252383/1‑47 (MQ=255) gCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:3639030/45‑1 (MQ=255) gCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGc > 1:866859/1‑71 (MQ=255) ttCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:1838680/1‑45 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCa > 1:2707584/1‑36 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGc > 1:3163876/1‑41 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:3159334/1‑42 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:444015/1‑42 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:2039151/1‑42 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt > 1:2638871/1‑71 (MQ=255) ccaGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTg > 1:2298655/3‑71 (MQ=255) ccaGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTg > 1:573383/3‑71 (MQ=255) cGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt < 1:3383346/70‑1 (MQ=255) gAGCATCGAATTGCCCATGTTGTTAGGCGTGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc < 1:1053910/71‑1 (MQ=255) gAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc > 1:3029943/1‑71 (MQ=255) aGCATCGAATTGCCCATGTTGTTAAGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGcc < 1:2289205/71‑1 (MQ=255) gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCg < 1:902106/71‑1 (MQ=255) cATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGct < 1:959661/37‑2 (MQ=16) cATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc < 1:3393915/68‑1 (MQ=255) tCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGttaa > 1:3008743/1‑55 (MQ=255) tCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg < 1:1649990/56‑1 (MQ=255) tCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg < 1:3627368/56‑1 (MQ=255) gAATTGCCCATGTTGTTACGCTCGTCACCTGCATAGACCAGGGTCATTTCGTTGAACGCTTTGCCGGGCaa < 1:4103696/71‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:1329128/1‑35 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGaa > 1:547197/1‑56 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:598495/70‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa > 1:481889/1‑70 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:2921634/70‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:132593/70‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGAGTCATTTCGTTGAACGCTTTGCCGGGCa > 1:2397820/1‑70 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACAAGCGTCATTTCGTTGAACGCTTTGCCGGGCa > 1:93207/1‑70 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:393616/1‑42 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:1146533/1‑42 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGc > 1:1354108/1‑67 (MQ=255) | GGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAA > minE/2919115‑2919251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |