| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,691 | T→C | 8.2% | T394T (ACA→ACG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,691 | 0 | T | C | 8.2% | 235.4 / 10.2 | 85 | T394T (ACA→ACG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (41/37); new base C (2/5); total (43/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACG > minE/311621‑311760 | gCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAtt > 1:1042670/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:249664/1‑71 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc < 1:1421849/71‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCTAGCACCATCCCGAACATTGTTAGc < 1:866525/69‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc < 1:2887343/69‑1 (MQ=255) tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGtt > 1:4134395/1‑71 (MQ=255) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga < 1:2056628/70‑3 (MQ=9) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa < 1:1212382/60‑1 (MQ=255) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGttt < 1:1844710/68‑1 (MQ=255) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa < 1:1766885/69‑1 (MQ=255) acaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:3972220/71‑1 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc > 1:2141354/1‑68 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCACGAACATTGTTAGCGTGttt > 1:1351879/1‑65 (MQ=255) aaCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga < 1:2470879/71‑1 (MQ=255) aCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag > 1:988806/1‑69 (MQ=255) aCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag > 1:2723166/1‑69 (MQ=255) gATGGCGTCATCCACCATCAGGCCGATGGCGAGCTCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAg < 1:4105831/70‑1 (MQ=255) gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa < 1:2358204/67‑1 (MQ=9) gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:3728942/4‑70 (MQ=9) tCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGtt < 1:1742676/45‑1 (MQ=12) cATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTa < 1:2515136/54‑1 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa > 1:304189/1‑44 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTa > 1:3510059/1‑44 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg < 1:78444/46‑1 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag > 1:1643518/1‑55 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc > 1:1610436/1‑69 (MQ=255) caccaACAGGCCGATGGCAAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc > 1:2142968/1‑69 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg > 1:3044013/1‑71 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAg > 1:389126/1‑38 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:1331522/1‑71 (MQ=255) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag < 1:2436159/50‑1 (MQ=255) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACg > 1:176189/1‑71 (MQ=255) cAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg > 1:1848640/1‑40 (MQ=255) cAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGc > 1:420902/1‑53 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgag < 1:779333/48‑1 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa < 1:1791344/67‑1 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc > 1:3299922/1‑70 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc > 1:190568/1‑70 (MQ=255) gcccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGtt < 1:648351/38‑1 (MQ=21) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:3879823/1‑56 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:93436/1‑56 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1963441/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2469561/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:2540081/71‑1 (MQ=255) gCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1182417/71‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaaa < 1:3136104/53‑1 (MQ=12) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:2602027/69‑1 (MQ=255) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa > 1:2581942/1‑69 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa > 1:2716595/1‑68 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:934981/71‑1 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:774543/1‑71 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:2415528/71‑1 (MQ=255) gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:2622223/60‑1 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGt < 1:2688022/71‑1 (MQ=255) ggggAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:1622186/4‑71 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:3103408/1‑67 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg > 1:2852074/1‑67 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:1548159/1‑71 (MQ=255) aGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTccctaa > 1:2973426/1‑68 (MQ=12) gCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAg < 1:2314351/44‑1 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa < 1:1970270/71‑1 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa < 1:3817477/71‑1 (MQ=255) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCa < 1:2346159/69‑1 (MQ=255) cATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAAt < 1:3493206/70‑1 (MQ=255) aTCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:2067735/1‑40 (MQ=255) aTCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:1158617/1‑40 (MQ=255) aTCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:3587175/66‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:573019/1‑48 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:1937508/54‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:3905647/53‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAgg < 1:2078536/57‑1 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATac > 1:697547/1‑70 (MQ=255) cccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATacca > 1:3915903/1‑71 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCaa > 1:1206526/1‑43 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:1870261/1‑46 (MQ=255) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa > 1:3004284/1‑65 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCa < 1:4087896/39‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCa < 1:2427382/39‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg > 1:2811118/1‑70 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg < 1:3528148/70‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGt < 1:2309965/71‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:4055097/39‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCaa > 1:1072439/1‑60 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAAt > 1:798581/1‑61 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTa > 1:2668064/1‑71 (MQ=255) aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc > 1:1860586/1‑71 (MQ=255) ttGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACg < 1:716954/71‑1 (MQ=255) ttGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACg < 1:1957162/71‑1 (MQ=255) | GCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACG > minE/311621‑311760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |