| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,207 | A→G | 18.0% | G163G (GGT→GGC) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,207 | 0 | A | G | 18.0% | 201.9 / 38.5 | 89 | G163G (GGT→GGC) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (41/32); new base G (8/8); total (49/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
CCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCG > minE/2919137‑2919277 | ccAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCa < 1:865859/71‑1 (MQ=255) aGACGCAAATCCAGACCGGTAAGCGCCGCATCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAcc > 1:636375/1‑71 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:2070343/71‑1 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg < 1:3903685/71‑1 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGTGCGTCACCTGCa > 1:3907997/1‑70 (MQ=255) cAAATCCAGACCGGTAAGAGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:1794130/1‑70 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa < 1:3379729/71‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:2544179/66‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1135382/66‑1 (MQ=255) tCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:1816826/66‑1 (MQ=255) cAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGAcc < 1:2526445/70‑1 (MQ=255) cGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTc < 1:714916/71‑1 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt > 1:2721947/1‑71 (MQ=255) gTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt < 1:3430471/71‑1 (MQ=255) tcaGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCAtc > 1:3373137/3‑70 (MQ=255) aGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc > 1:73158/1‑71 (MQ=255) aGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTc > 1:2368877/1‑71 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCa < 1:1818742/60‑1 (MQ=255) gcgcCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCa < 1:1950686/60‑1 (MQ=255) cgccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:3649841/1‑63 (MQ=255) ccgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGtt > 1:929025/1‑70 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCAcc < 1:3324485/45‑1 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa < 1:1296743/71‑1 (MQ=255) cgcAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGa < 1:2055691/71‑1 (MQ=255) cAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:632986/1‑47 (MQ=255) cAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:1252383/1‑47 (MQ=255) gCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa < 1:3639030/45‑1 (MQ=255) gCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGc > 1:866859/1‑71 (MQ=255) ttCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:1838680/1‑45 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCa > 1:2707584/1‑36 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGc > 1:3163876/1‑41 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:2039151/1‑42 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:444015/1‑42 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:3159334/1‑42 (MQ=255) tCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt > 1:2638871/1‑71 (MQ=255) ccaGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTg > 1:2298655/3‑71 (MQ=255) ccaGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTg > 1:573383/3‑71 (MQ=255) cGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCttt < 1:3383346/70‑1 (MQ=255) gAGCATCGAATTGCCCATGTTGTTAGGCGTGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc < 1:1053910/71‑1 (MQ=255) gAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc > 1:3029943/1‑71 (MQ=255) aGCATCGAATTGCCCATGTTGTTAAGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGcc < 1:2289205/71‑1 (MQ=255) gCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCg < 1:902106/71‑1 (MQ=255) cATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGct < 1:959661/37‑2 (MQ=16) cATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGc < 1:3393915/68‑1 (MQ=255) tCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGttaa > 1:3008743/1‑55 (MQ=255) tCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg < 1:1649990/56‑1 (MQ=255) tCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTg < 1:3627368/56‑1 (MQ=255) gagttGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCa < 1:1098310/67‑1 (MQ=255) gAATTGCCCATGTTGTTACGCTCGTCACCTGCATAGACCAGGGTCATTTCGTTGAACGCTTTGCCGGGCaa < 1:4103696/71‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATa > 1:1329128/1‑35 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGaa > 1:547197/1‑56 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:2921634/70‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa > 1:481889/1‑70 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:132593/70‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCa < 1:598495/70‑1 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGAGTCATTTCGTTGAACGCTTTGCCGGGCa > 1:2397820/1‑70 (MQ=255) gAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACAAGCGTCATTTCGTTGAACGCTTTGCCGGGCa > 1:93207/1‑70 (MQ=255) gttGCCCATGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGtta < 1:3533291/51‑2 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:393616/1‑42 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:1146533/1‑42 (MQ=255) aTTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGc > 1:1354108/1‑67 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCa > 1:1323160/1‑43 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCa > 1:1934258/1‑43 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATg > 1:110867/1‑71 (MQ=255) ttGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATg > 1:1127789/1‑71 (MQ=255) gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg > 1:635454/1‑62 (MQ=255) gCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCgg > 1:2797244/1‑62 (MQ=255) cATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAt > 1:1138474/1‑39 (MQ=255) aTGTTGTTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTg < 1:2449036/71‑1 (MQ=255) aTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGc < 1:3455239/66‑1 (MQ=255) aTGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTg < 1:2386096/71‑1 (MQ=255) tgttgtTGCGCGCATCGCCCGCGTAGACCAGCGTc < 1:2322340/35‑1 (MQ=12) tgttgtTGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTg > 1:2568003/1‑70 (MQ=255) tgttgtTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTg > 1:1100509/1‑70 (MQ=255) gttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCt > 1:398805/1‑68 (MQ=255) ttgttgCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGttaa > 1:3163357/1‑41 (MQ=16) ttgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTc > 1:1663463/1‑65 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa > 1:364041/1‑69 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa > 1:359370/1‑69 (MQ=255) tgttACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATg > 1:2263890/1‑71 (MQ=255) gttGCGCGCATCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATgg > 1:94299/1‑71 (MQ=255) tACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGt > 1:2504116/1‑70 (MQ=255) aCGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCa > 1:2431715/1‑65 (MQ=255) cgcgcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag < 1:1368559/71‑1 (MQ=255) cgcgcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag < 1:1267869/71‑1 (MQ=255) cgcgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGtcat < 1:2559838/71‑4 (MQ=255) cgcgcaTCGCCCGCGTAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGtcat < 1:649083/71‑4 (MQ=255) gcgcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTg < 1:2697759/61‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTg < 1:915770/42‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTg < 1:3266232/42‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag > 1:41487/1‑68 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCaaa > 1:3445416/1‑46 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc < 1:3590668/70‑1 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc < 1:331130/70‑1 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg > 1:789079/1‑71 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg > 1:2325574/1‑71 (MQ=255) | CCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCG > minE/2919137‑2919277 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |