| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,269 | G→T | 7.6% | L143I (CTC→ATC) ‡ | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,269 | 0 | G | T | 7.6% | 259.2 / 5.0 | 78 | L143I (CTC→ATC) ‡ | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base G (38/34); new base T (4/2); total (42/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACG > minE/2919199‑2919330 | cgcgcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag < 1:1267869/71‑1 (MQ=255)cgcgcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag < 1:1368559/71‑1 (MQ=255) gcgTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTgag > 1:41487/1‑68 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc < 1:331130/70‑1 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATcc < 1:3590668/70‑1 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg > 1:2325574/1‑71 (MQ=255) aCCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCg > 1:789079/1‑71 (MQ=255) cTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGcc < 1:1040683/71‑1 (MQ=255) gCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcag > 1:1286198/1‑71 (MQ=255) cATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcag > 1:3527105/1‑70 (MQ=255) cATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCcag > 1:4013411/1‑70 (MQ=255) gtAGACCAGCGTCATCTCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCcagca < 1:2285979/70‑1 (MQ=255) aGACCAGAGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCagcagc > 1:2015197/1‑71 (MQ=255) aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg > 1:2004543/1‑71 (MQ=255) aCCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTg < 1:2004264/71‑1 (MQ=255) cAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCg < 1:85451/71‑1 (MQ=255) gCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTg > 1:1666852/1‑71 (MQ=255) gCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTg < 1:1871423/71‑1 (MQ=255) cGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTgg < 1:2751769/71‑1 (MQ=255) cGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTgg > 1:789520/1‑71 (MQ=255) gTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTgg > 1:3600264/1‑70 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATg < 1:3168188/71‑1 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATg > 1:990945/1‑71 (MQ=255) aTTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTCGGATg > 1:2055453/1‑71 (MQ=255) tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaaga < 1:497683/47‑1 (MQ=255) tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaag < 1:307900/46‑1 (MQ=255) tttCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg < 1:2113160/71‑1 (MQ=255) tCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGAt > 1:2744971/1‑67 (MQ=255) tCGTTAAACGCCTTGCCCGGCAGGTGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGaa > 1:2685177/1‑71 (MQ=255) cGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaa > 1:3043588/1‑42 (MQ=255) cGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaa > 1:485610/1‑42 (MQ=255) cGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaa > 1:733461/1‑42 (MQ=255) gTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGagaa > 1:956752/1‑41 (MQ=255) gTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTgg > 1:2624806/1‑62 (MQ=255) ttGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTc < 1:3418106/71‑1 (MQ=255) ttGAACGCTTTGCCGGGCAAATGCTCCTCCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTc < 1:2043968/71‑1 (MQ=255) tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa > 1:2506129/1‑67 (MQ=255) tGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa > 1:1535395/1‑67 (MQ=255) gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa < 1:2427816/70‑1 (MQ=255) gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt > 1:2388981/1‑71 (MQ=255) gAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCAt > 1:1358819/1‑71 (MQ=255) aaCGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa < 1:2860077/65‑1 (MQ=255) aCGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTc > 1:2311023/1‑67 (MQ=255) gCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTTGGATGGAACTCATTGGt < 1:932480/71‑1 (MQ=255) cTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCa < 1:2773059/65‑1 (MQ=255) cTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTgg > 1:3077225/1‑69 (MQ=255) cTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTg > 1:1958010/1‑68 (MQ=255) tttGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGt > 1:3385894/1‑69 (MQ=255) ttGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAg > 1:1611566/1‑71 (MQ=255) tGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCa < 1:974414/69‑1 (MQ=255) tGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAgg > 1:2211170/1‑71 (MQ=255) ccGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGcc < 1:757283/71‑1 (MQ=255) cGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCa > 1:3480953/1‑66 (MQ=255) cGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCa > 1:2964642/1‑66 (MQ=255) ggCAAATGCTCCTGCAT‑GTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc < 1:545322/71‑1 (MQ=255) gCAAATGCTCCTGCATTGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc < 1:2475523/71‑1 (MQ=255) gCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc < 1:3169777/71‑1 (MQ=255) gCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc < 1:2361766/71‑1 (MQ=255) cAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTc > 1:2085531/1‑70 (MQ=255) aggtGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCa > 1:98435/4‑71 (MQ=255) aaaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTg > 1:3569019/1‑57 (MQ=255) aaaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTcc < 1:1389860/70‑1 (MQ=255) aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg < 1:582538/47‑1 (MQ=255) aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg < 1:2956864/47‑1 (MQ=255) aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg < 1:1632614/47‑1 (MQ=255) aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCa < 1:2202348/70‑1 (MQ=255) aaTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCa > 1:1553601/1‑70 (MQ=255) aTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTgg < 1:425009/56‑1 (MQ=255) aTGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTgg < 1:228071/56‑1 (MQ=255) tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCa > 1:2689462/1‑68 (MQ=255) tGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATAc < 1:1137237/71‑1 (MQ=255) tGCTCCTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAgcc > 1:1982011/1‑59 (MQ=255) gCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACt < 1:1621294/48‑1 (MQ=255) gCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCATGCCATTCCATAcc > 1:647500/1‑71 (MQ=255) cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCgg < 1:54855/69‑1 (MQ=255) cTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGc < 1:2539398/70‑1 (MQ=255) cTGCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGTTGGTCAGCCCGTTCCACACCgg < 1:1283027/69‑1 (MQ=255) tGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATgg < 1:3690060/39‑1 (MQ=255) gCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCAc > 1:3675509/1‑70 (MQ=255) gCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACg > 1:2395215/1‑71 (MQ=255) gCATGGTCATCAGGTCCGCCAGCAGCTGGGTCGGGTGGAACTCGtt > 1:1988023/1‑46 (MQ=12) cATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa > 1:321127/1‑39 (MQ=255) cATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa > 1:1828206/1‑39 (MQ=255) cATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa > 1:4136648/1‑39 (MQ=255) cATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCAc < 1:1229525/69‑1 (MQ=255) gTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGaa > 1:716041/1‑35 (MQ=255) gTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGc > 1:2418342/1‑49 (MQ=255) agaagaTCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCa > 1:3191303/1‑53 (MQ=255) | CGCGCGTCACCTGCATAGACCAGCGTCATTTCGTTGAACGCTTTGCCGGGCAAATGCTCCTGCATGGTGAGAAGATCCGCCAGCAGCTGCGTGGGATGGAACTCATTGGTCAGGCCATTCCATACCGGCACG > minE/2919199‑2919330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |