Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 478,221 C→G A103A (GCC→GCG)  cydA → cytochrome d terminal oxidase, subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE478,2210CG99.8% 804.2 / ‑5.9 440A103A (GCC→GCG) cydAcytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (0/439);  minor base T (0/1);  total (0/440)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 440 total aligned reads displayed.

CGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTC  >  minE/478196‑478257
                         |                                    
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:554760/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:667822/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:655136/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:651386/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:640944/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:635897/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:62267/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:615236/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:593126/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:588240/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:571536/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:68757/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:548756/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:539836/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1004005/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:518958/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:513235/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:489708/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:481709/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:45878/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:44041/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:825168/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:989835/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:983706/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:954877/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:935540/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:921978/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:912503/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:878678/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:874569/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:860180/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:84422/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:530249/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:813025/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:805132/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:800656/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:782571/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:768973/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:755179/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:75137/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:718796/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:696634/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:288598/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:129444/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:127102/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1139410/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:192711/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1130359/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1111590/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1087545/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:225287/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:24353/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:257121/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:277481/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:29559/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:330878/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:379196/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:43078/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:435550/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:418352/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:102887/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1036881/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1058118/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1052256/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:353987/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:347988/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGtccttc  <  1:562973/62‑1 (MQ=12)
cgcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGACCTGttcttc  <  1:1059346/62‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAGGCGACCTTCGTCGGCCTGttcttc  <  1:155896/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1069584/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:885267/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:961674/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:831189/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:968344/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:820737/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1011971/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:1058410/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:915493/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:174943/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:472225/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:504045/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:391566/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:385266/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:360234/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:341218/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:302983/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:610337/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:270427/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:234422/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:67851/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:211210/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:20217/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:736033/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:739255/61‑1 (MQ=12)
 gcTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:162648/61‑1 (MQ=12)
  cTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:8931/60‑1 (MQ=12)
  cTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:943666/60‑1 (MQ=12)
  cTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:727234/60‑1 (MQ=12)
  cTGGCCATCGAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:40647/60‑1 (MQ=12)
           gAAGGCCTGATGGCGTTCTTCCTCGAAGCGACCTTCGTCGGCCTGttcttc  <  1:851886/51‑1 (MQ=2)
                         |                                    
CGCTGGCAATCGAAGGTCTGATGGCCTTCTTCCTCGAATCCACCTTTGTAGGTCTGTTCTTC  >  minE/478196‑478257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑