| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,565,310 | 3 bp→AAG | intergenic (+6/+4) | yibT → / ← mtlR | hypothetical protein/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,565,310 | 0 | C | A | 99.8% | 1634.4 / ‑4.8 | 562 | intergenic (+6/+6) | yibT/mtlR | hypothetical protein/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (561/0); minor base T (1/0); total (562/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
| * | minE | 2,565,311 | 0 | G | A | 100.0% | 1634.8 / NA | 562 | intergenic (+7/+5) | yibT/mtlR | hypothetical protein/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (562/0); total (562/0) | |||||||||||
| * | minE | 2,565,312 | 0 | T | G | 99.8% | 1656.7 / NA | 562 | intergenic (+8/+4) | yibT/mtlR | hypothetical protein/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (561/0); minor base C (1/0); total (562/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 562 total aligned reads displayed.
CGGCTTTATCGGCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTT > minE/2565269‑2565332 ||| cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTATGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:222961/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAgg > 1:234206/3‑60 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:1007711/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:827567/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:590248/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:578258/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:254466/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:551108/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGc > 1:961440/3‑61 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:702547/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:711342/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:720898/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:517497/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:691187/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:680192/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:669194/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:732439/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:65246/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:645797/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:637413/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:624465/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:616236/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:611477/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:60166/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:56487/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:560026/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:539608/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:529710/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1012573/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:863204/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:98199/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:97680/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:967695/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:949454/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:942130/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:936566/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:920342/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:915196/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:899703/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:879496/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:871106/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:734553/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:855336/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:850811/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:83925/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:819973/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:810777/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:789020/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:780129/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:76600/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:760134/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:750688/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1128313/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:268421/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:260257/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:246278/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:19432/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:184592/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:176791/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:169882/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:157319/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:147761/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:130765/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:115735/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:488885/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1119399/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1109590/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1103944/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1096540/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1089770/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1085284/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1070524/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1066697/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1058498/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1034651/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:1024840/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:281251/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:503682/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:481718/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:465458/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:446452/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:431939/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:423213/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:414956/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:401217/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:391678/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:384370/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:373997/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:367326/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:357117/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:347092/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:340727/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:331719/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:328189/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:320890/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:308721/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:292093/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:288203/3‑62 (MQ=255)cagCTTTATCGTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:278860/3‑62 (MQ=255)cagCTTTATCCTCCGAAACAGGTAGAGGAGAAGTAAGC‑GAAAGTACTTACTTTTGAAAGGCt > 1:987432/3‑62 (MQ=255) ||| CGGCTTTATCGGCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTT > minE/2565269‑2565332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |