Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,487,214 G→T P33Q (CCG→CAG)  nuoN ← NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,487,2140GT84.3% 962.8 / 261.1 470P33Q (CCG→CAG) nuoNNADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base G (74/0);  new base T (396/0);  total (470/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 470 total aligned reads displayed.

ATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCC  >  minE/1487198‑1487259
                |                                             
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTc                  >  1:833357/1‑46 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCg                 >  1:716705/1‑47 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:563743/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:694180/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:667359/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:661964/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:645087/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:631134/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:622415/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:618895/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:594842/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:586339/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:575992/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:713741/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:548622/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:535021/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:528990/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:508825/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:488877/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:477240/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:46949/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:462290/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:455703/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:809129/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:94604/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:931433/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:90745/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:89541/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:884435/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:864808/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:856153/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:850620/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:824978/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:705216/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:795693/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:792902/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:790328/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:768324/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:754169/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:741926/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:733313/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:729871/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:112801/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:229815/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:328170/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:325049/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:448433/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:310409/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:30385/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:299228/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:290360/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:27259/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:266285/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:253025/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:242730/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:319221/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:215875/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:20829/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:196673/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:185966/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:177419/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:173003/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:152811/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:13870/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:132900/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:124875/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:102265/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:335029/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:434761/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:421800/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:413510/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:404003/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:393399/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:391001/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:382348/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:368746/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:362042/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:353456/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:347679/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:337950/1‑62 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGc   >  1:654435/1‑61 (MQ=255)
ataGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACAAGcc  >  1:519801/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGc                    >  1:779389/1‑44 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:630371/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:438663/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:915383/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:431745/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:684902/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:871528/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:512827/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:410650/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:280565/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:816579/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:627222/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:222900/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:725157/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:775713/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:589288/1‑62 (MQ=255)
ataGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGcc  >  1:60554/1‑62 (MQ=255)
aaaGCCTTCAAGCCACTGGTAGGCGAAAGTACAGGTGGCGAGCCTCGCCAACAATACCAg    >  1:6340/3‑60 (MQ=255)
                |                                             
ATAGCCTTCAAGCCACGGGTAGGCGAAAGTACAGGTGGCGAGGCTCGCCAACAATACCAGCC  >  minE/1487198‑1487259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑