Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	177,202	Δ1 bp	coding (450/2433 nt)	yaeT →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	177,202	0	C	.	100.0%	100.5 / NA	24	coding (450/2433 nt)	yaeT	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/0);  total (24/0)

CAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTG  >  minE/177178‑177239                         |                                      cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:336541/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:980182/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:976774/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:907278/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:871141/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:76607/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:673132/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:636122/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:540186/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:472988/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:41400/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:355800/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:1014355/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:326442/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:295875/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:1848308/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:1627802/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:1411637/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:134524/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:1252328/1‑61 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgt   >  1:1631907/1‑60 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgt   >  1:1199684/1‑60 (MQ=255) cAGCGTCGGTAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCg    >  1:1231023/1‑59 (MQ=255) cAGCGTCGATAAATATAGCGCCAG‑GTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCgtg  >  1:1401369/1‑61 (MQ=255)                         |                                      CAGCGTCGGTAAATATAGCGCCAGCGTAAAAGCTGTCGTGACCCCGCTGCCGCGCAACCGTG  >  minE/177178‑177239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑