Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 862,345 +A coding (172/882 nt) yciV → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE862,3421.A100.0% 387.5 / NA 116E57K (GAA→AAA) yciVconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/116);  total (0/116)

Only 100 of 116 total aligned reads displayed.

CTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCC  >  minE/862319‑862379
                        |                                     
cTGCAATCGCGCCAGCAAGATAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:647098/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCTTTGAATCTTATTcc  <  1:1054209/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:29168/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:495249/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:470270/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:439080/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:428757/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:425307/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:398221/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:397344/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:38799/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:380324/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:376702/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:361290/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:524814/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:285757/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:261158/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:255226/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:251599/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:232698/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:223260/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:209808/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:20442/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:200913/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:194396/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1016819/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:707866/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:967986/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:960540/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:939976/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:922402/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:904165/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:886206/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:844515/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:829674/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:826937/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:779342/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:774550/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:504482/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:681546/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:658673/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:634396/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:62924/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:608937/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:551390/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:548112/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:547430/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:541817/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:528270/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1893189/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1159896/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1878860/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1402466/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1364417/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1319477/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1286349/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1246107/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1211894/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1204528/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1179343/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1175867/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1162916/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1420714/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1153429/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1141149/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1101555/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1069958/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1062277/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1059086/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1049356/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1041296/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1029834/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1019910/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1628421/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1855101/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1834046/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1751407/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1866077/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1720363/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1662866/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1662358/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1650456/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1644692/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1453648/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:159892/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1579304/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1514719/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1473602/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1462269/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:187418/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATATTATTcc  <  1:1609226/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTCGCGTTGAATCTTATTcc  <  1:1482631/62‑1 (MQ=255)
cTGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACCGGCGTTGAATCTTATTcc  <  1:556426/62‑1 (MQ=255)
 ttcAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:30495/59‑1 (MQ=255)
 tGCTATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1389331/61‑1 (MQ=255)
 tGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:163948/61‑1 (MQ=255)
 tGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1026602/61‑1 (MQ=255)
 tGCAATCGCGCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1425689/61‑1 (MQ=255)
       cgcgCCAGCAAGAGAGGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1739831/55‑1 (MQ=255)
                   agagGAAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTcc  <  1:1554492/43‑1 (MQ=38)
                        |                                     
CTGCAATCGCGCCAGCAAGAGAGGAAATTTCACGTTCGGGACTGGCGTTGAATCTTATTCC  >  minE/862319‑862379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: