Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,687,817	+G	coding (462/1623 nt)	pck ←	phosphoenolpyruvate carboxykinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,687,814	1	.	G	100.0%	102.1 / NA	28	P155P (CCG→CCC)	pck	phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (0/28);  total (0/28)

AGTTCTTCATCGCTC‑GGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAC  >  minE/2687800‑2687858                |                                             acttcttcATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1045325/58‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAGAATGCGCCTGCCAGGCCAc  <  1:1380402/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGGCAc  <  1:269514/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1625780/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:762067/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:739528/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:637878/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:474367/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:46412/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:436689/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:262567/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:219003/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1886179/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1858171/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1716351/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1689874/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:157248/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1490260/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:141863/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1407042/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1388978/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:137182/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1229351/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1153283/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1141487/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1113741/60‑1 (MQ=255) aGTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1065742/60‑1 (MQ=255)  gTTCTTCATCGCTCGGGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAc  <  1:1407251/59‑1 (MQ=255)                |                                             AGTTCTTCATCGCTC‑GGGCGAATAAACATGTTTTTGACAAAATGCGCCTGCCAGGCCAC  >  minE/2687800‑2687858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑