Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE419,6991.A26.7% 438.9 / 236.8 210intergenic (+122/+32)ubiF/glnX2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol oxygenase/tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base . (153/1);  new base A (0/56);  total (153/57)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-21
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

AGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACG  >  minE/419649‑419743
                                                   |                                             
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGCACGCAGAAAAGCAAAAAGCTCg                                    >  1:156828/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTc                                     >  1:314099/1‑60 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTc                                     >  1:1842286/1‑60 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:478331/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:79032/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1276949/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:212439/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:778564/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1317347/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:756088/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1813822/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1454154/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:358334/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1708465/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:483617/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1549377/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1609074/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:585041/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:497346/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1085883/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1244806/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:795604/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1217764/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1199924/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1099969/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1048872/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:811324/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:828841/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:404383/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1248548/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:850377/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:1116243/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCg                                    >  1:284825/1‑61 (MQ=255)
aGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAAAGCAGAAAAGCAAAAAGCt                                      >  1:1557291/1‑59 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:356629/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1398199/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:175685/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:363502/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1864884/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:469354/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:436449/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:765307/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1024839/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:96961/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:939564/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1108850/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:89914/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1169900/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:788962/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1290146/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:528161/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:136374/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:736077/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:731011/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:676356/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:657117/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:622028/62‑1 (MQ=255)
                        ttCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:5597/62‑1 (MQ=255)
                         tCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1896047/61‑1 (MQ=255)
                         tCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1373042/61‑1 (MQ=255)
                         tCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1304483/61‑1 (MQ=255)
                         tCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1661322/61‑1 (MQ=255)
                         tCGACATTGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:1273323/61‑1 (MQ=255)
                         tCGACATCGAATGAACGCAGAAAAGCAAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTgg            <  1:690558/61‑1 (MQ=38)
                               ttcaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1258257/4‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCt                        >  1:1442062/1‑42 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTgggg      >  1:1193086/1‑60 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1236359/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1392277/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:749581/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1319039/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:79433/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:69442/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1136049/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1111031/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:916988/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1058955/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:952574/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1034717/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:986859/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1686812/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:379785/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:385087/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:387302/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:256439/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:227522/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1008542/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1734026/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:51586/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1649891/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1627816/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1623277/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:616095/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1509252/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1484633/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:1464428/1‑61 (MQ=255)
                               ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGt     >  1:688435/1‑61 (MQ=255)
                               ttGAATGAACGC‑‑AAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACg  >  1:843554/1‑62 (MQ=38)
                               ttGAAAGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTgggg      >  1:1189806/1‑60 (MQ=255)
                                              aaaGCATAAAAGCTCTCAGAAGCTTGCTTTTTTAATGTgg            <  1:350705/40‑1 (MQ=255)
                                                   |                                             
AGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACG  >  minE/419649‑419743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: