Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE426,6600A.28.3% 868.8 / inf 364coding (253/801 nt)nagBglucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/261);  new base . (103/0);  total (103/261)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 457 total aligned reads displayed.

ATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGT  >  minE/426637‑426711
                       |                                                   
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGctc                                      >  1:1110888/1‑38 (MQ=38)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:986475/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:1658306/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:1704141/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:1732034/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:175575/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:183706/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:1842517/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:205478/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:420814/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:661530/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:1058528/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:898169/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:79806/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:1156311/1‑54 (MQ=255)
aTTACGGTGCATAAAGCTGTAGT‑GCTTTCCGGATGCTCTTTCGGCAGACCGACa                      >  1:678445/1‑54 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:280219/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1860289/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:966436/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1784921/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:789257/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1868120/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:22285/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:237630/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:300081/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:314898/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:360671/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:37219/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:735407/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:494773/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:505691/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:547358/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:59433/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:637970/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:705622/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1094064/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1640766/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1128593/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1619561/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1205865/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1241521/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1251941/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1325101/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1478372/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:1546315/62‑1 (MQ=255)
             aaGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGGCCATGTTGAAGGt  <  1:1690264/62‑1 (MQ=255)
               gCTGTGGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:951822/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCTGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:457636/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:172097/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1569436/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:476738/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1309082/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:530181/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:571532/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:6192/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:687914/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1188512/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1831042/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1122346/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:822845/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:847679/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:878999/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:891353/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1676534/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:930562/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:973883/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1803634/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1794848/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:292316/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1880732/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1516557/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1419814/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:326073/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1825348/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:408836/53‑1 (MQ=255)
               gCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1348001/53‑1 (MQ=255)
                cTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1290165/52‑1 (MQ=255)
                cTGTAGTAGCTTCCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGt         <  1:1493341/52‑1 (MQ=255)
                    agtagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  <  1:440512/55‑1 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTg       >  1:1744275/1‑48 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAgg   >  1:37875/1‑52 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1029975/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1453875/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:233866/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1002367/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1527781/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1070444/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:754325/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:748828/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1170208/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:699084/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1231256/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1587272/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1412852/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:177208/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1404763/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:562166/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1271132/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:339343/1‑53 (MQ=255)
                      tagCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGt  >  1:1375062/1‑53 (MQ=255)
                       |                                                   
ATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGT  >  minE/426637‑426711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑