Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,411,889	0	C	A	67.7%	25.3 / 26.8	31	Q9H (CAG→CAT)	rsuA	16S rRNA pseudouridylate 516 synthase
Reads supporting (aligned to +/- strand):  ref base C (10/0);  new base A (0/21);  total (10/21)
Fisher's exact test for biased strand distribution p-value = 2.25e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGA  >  minE/1411842‑1411942                                                |                                                      ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAttt                                         >  1:1059333/1‑62 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAttt                                         >  1:666364/1‑62 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAttt                                         >  1:552676/1‑62 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAttt                                         >  1:338894/1‑62 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAttt                                         >  1:1805003/1‑62 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAttt                                         >  1:1617787/1‑62 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAtt                                          >  1:1880040/1‑61 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAtt                                          >  1:1640105/1‑61 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAtt                                          >  1:1498986/1‑61 (MQ=255) ttGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAAtt                                          >  1:1479653/1‑61 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:961267/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1636794/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:784523/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:75469/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:732244/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:712825/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:689710/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1090100/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1114238/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:484382/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:411902/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1258129/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:316634/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:253334/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:212935/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:210677/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:104445/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1844966/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1771491/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1722736/62‑1 (MQ=255)                                        ccGAGTTGATGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGa  <  1:1698576/62‑1 (MQ=255)                                                |                                                      TTGCCGCGGATTTCACGCCCGGCAATAGCACGGCTAACGCCGAGTTGCTGTGCGATAAATTTATCAAGTCGCATGTGTGTGATTTTGCCTGTAAAAACGGA  >  minE/1411842‑1411942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑