Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,376,832	T→C	N496S (AAC→AGC)	mglA ←	fused methyl‑galactoside transporter subunitsl and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,376,832	0	T	C	100.0%	84.2 / NA	23	N496S (AAC→AGC)	mglA	fused methyl‑galactoside transporter subunitsl and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/23);  total (0/23)

CTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCG  >  minE/1376778‑1376838                                                       |       cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1486398/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:715617/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:666194/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:293845/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:29287/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:197155/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1849936/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1842470/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1787410/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:161121/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1596214/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1548261/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1031712/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1463172/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:142135/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1392458/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1370298/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:122996/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1167551/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1041362/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1034677/61‑1 (MQ=255) cTCATGGGAAGCCCCTAATCTTAAAGGTACAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1216711/61‑1 (MQ=255)                           gTGCAAAGACGCAAGACGCAGAATTTCGCTTTGCg  <  1:1333282/35‑1 (MQ=255)                                                       |       CTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCG  >  minE/1376778‑1376838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑