| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,345,189 | C→A | intergenic (+445/+6) | glnG → / ← hemN | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)/coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,345,189 | 0 | C | A | 100.0% | 10.6 / NA | 5 | intergenic (+445/+6) | glnG/hemN | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)/coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) | |||||||||||
CTCAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAA > minE/2345137‑2345198 | ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1081627/62‑1 (MQ=255)ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1250288/62‑1 (MQ=255)ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1673360/62‑1 (MQ=255)ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1720659/62‑1 (MQ=255)ctcAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTAACTATTTaa < 1:1743658/62‑1 (MQ=255) | CTCAGCACATTGATGGCGCTTTCGTTTTCTACTTTGTAAACGAAGCGCCATTCACTATTTAA > minE/2345137‑2345198 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |