Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,427,572 A→C P303P (CCT→CCG rffE ← UDP‑N‑acetyl glucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,427,5720AC100.0% 33.1 / NA 10P303P (CCT→CCGrffEUDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/10);  total (0/10)

GACGCTCAGTGGTATCGCGCATCACCAGCACAGGTTTCCCCAGCGAAGGCGC  >  minE/2427541‑2427592
                               |                    
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:1332451/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:236259/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:240261/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:279156/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:292840/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:317166/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:619459/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:775737/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:786082/52‑1 (MQ=255)
gACGTTCGGTGGTGTCGCGCATCACCAGGACCGGCTTGCCGAGCGATGgcgc  <  1:916499/52‑1 (MQ=255)
                               |                    
GACGCTCAGTGGTATCGCGCATCACCAGCACAGGTTTCCCCAGCGAAGGCGC  >  minE/2427541‑2427592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: