| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 365,751 | G→C | V547V (GTC→GTG) | fepA ← | iron‑enterobactin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 365,751 | 0 | G | C | 99.8% | 1378.0 / ‑5.8 | 457 | V547V (GTC→GTG) | fepA | iron‑enterobactin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (0/456); minor base A (0/1); total (0/457) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 457 total aligned reads displayed.
CTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCC > minE/365721‑365768 | tGCGATAATCGTTACGGGACCAGGTCACGCCCGCCAGCCAacc < 1:110601/43‑1 (MQ=37) tGCGATAATCGTTACGGAACCAGGTCTCGCCCGCCAGCCAacc < 1:292324/43‑1 (MQ=255) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:271944/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:308284/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:304850/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:298694/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:296463/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:295021/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:286399/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:284961/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:282927/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:281034/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:279213/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:275761/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:310374/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:270291/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:266092/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:264371/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:259574/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:25617/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:253956/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:252108/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:249957/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:244495/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:242134/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:52983/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:91742/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:88732/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:82900/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:80679/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:78620/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:77660/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:74139/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:72032/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:69196/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:61491/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:58744/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:103918/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:51120/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:48701/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:46776/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:43849/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:42942/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:38314/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:36525/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:34583/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:318678/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:314518/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:140543/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:171210/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:168928/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:167894/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:166844/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:164715/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:160679/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:158613/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:149924/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:148044/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:145062/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:237410/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:136493/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:132830/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:13186/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:12713/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:123948/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:121204/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:115835/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:108195/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:107177/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:100178/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:200075/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:218064/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:224672/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:228252/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:233970/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:213344/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:212056/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:234439/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:206708/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:202890/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:239505/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:195026/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:193076/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:191684/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:189668/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:184436/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:181804/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:177086/43‑1 (MQ=38) tGCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:174625/43‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:302206/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:105814/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:9542/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:262267/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:64514/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:272675/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:197216/42‑1 (MQ=38) gCGATAATCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:155196/42‑1 (MQ=38) cGATAATCGTTACGTAACCAGGTCACGCCCGCCAGCCAacc < 1:117327/41‑1 (MQ=25) aaTCGTTAGGGAACCAGGTCACGCCCGCCAGCCAacc < 1:133915/37‑1 (MQ=25) aaTCGTTACGGAACCAGGTCACGCCCGCCAGCCAacc < 1:66390/37‑1 (MQ=37) | CTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCC > minE/365721‑365768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |