| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,144,422 | G→A | K231K (AAG→AAA) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,422 | 0 | G | A | 99.8% | 1713.9 / NA | 637 | K231K (AAG→AAA) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (0/636); minor base T (0/1); total (0/637) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 637 total aligned reads displayed.
CTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCC > minE/2144404‑2144466 | ttGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATc < 1:95811/61‑1 (MQ=255) ggtaTGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:206593/58‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTTCGCTTTGAGCAAGGTCATcc < 1:124159/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:313690/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:253576/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:310560/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:308890/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:307210/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:303842/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:299933/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:297204/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:294582/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:291729/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:288573/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:284570/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:283698/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:28295/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:279891/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:279186/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:275910/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:270508/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:267272/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:26379/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:260529/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:258429/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:256640/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:252538/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:61988/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:92380/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:88662/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:84453/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:81794/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:78901/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:76367/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:75105/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:72987/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:69702/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:65737/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:64946/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:314128/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:58806/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:55356/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:53882/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:4963/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:47075/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:44952/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:43108/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:411/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:38521/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:36764/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:32562/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:138599/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:100501/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:177881/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:174732/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:171434/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:168122/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:164576/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:162908/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:161921/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:157633/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:148631/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:142491/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:178837/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:136130/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:134527/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:132840/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:130496/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:127963/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:126174/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:123016/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:120074/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:115909/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:110869/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:102524/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:183812/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:24858/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:24658/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:242799/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:238441/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:236740/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:235318/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:230690/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:228488/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:226069/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:222674/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:220767/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:216451/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:213169/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:210349/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:203789/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:199768/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:194500/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:190460/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:18745/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:181696/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGAAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:223968/61‑1 (MQ=255) ggATGAGAGCGTGAAAATCCCGGCTGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:271731/60‑1 (MQ=255) ggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:109171/60‑1 (MQ=255) gATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:106925/59‑1 (MQ=255) | CTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCC > minE/2144404‑2144466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |