| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,144,455 | A→G | E242E (GAA→GAG) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,144,455 | 0 | A | G | 99.8% | 2009.7 / NA | 645 | E242E (GAA→GAG) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (0/643); total (0/645) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
Only 100 of 645 total aligned reads displayed.
CTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCC > minE/2144404‑2144466 | ttGGGATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATc < 1:95811/61‑1 (MQ=255) ggtaTGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:206593/58‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:310014/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:253576/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:308463/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:306640/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:303577/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:299615/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:296934/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:294574/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:291633/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:288413/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:284487/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:283664/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:282076/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:279773/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:278192/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:274542/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:271458/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:269089/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:267272/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:263788/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:260529/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:258429/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:256640/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:252538/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:58806/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:92380/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:88662/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:84453/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:80585/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:78901/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:76341/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:75091/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:71821/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:68930/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:65737/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:64656/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:61988/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:3135/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:55356/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:53684/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:4963/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:46884/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:44952/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:43108/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:40646/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:38521/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:35324/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:317989/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:31412/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:134527/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:100501/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:174868/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:168122/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:165071/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:163302/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:161921/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:157871/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:148631/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:143386/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:138599/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:136130/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:178057/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:132840/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:130496/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:128039/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:126224/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:124205/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:123148/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:120074/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:116309/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:110869/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:102524/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:222674/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:24858/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:24658/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:242799/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:237686/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:236792/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:235318/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:231073/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:228488/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:226069/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:224044/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:179975/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:220767/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:216451/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:213169/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:210349/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:203195/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:199768/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:194500/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:190460/61‑1 (MQ=255) gggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:181836/61‑1 (MQ=255) ggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:171978/60‑1 (MQ=255) ggATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:109171/60‑1 (MQ=255) gATGAGAGCGTGAAAATCCCGGCAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:106925/59‑1 (MQ=255) tagagCGTGAAAATCCCGGCAGAAGAAGTCAGAGTTCGCTTTGAGCAAGGTCATcc < 1:187862/55‑1 (MQ=255) cAGAAGAAGTCACAGTACGCTTTGAGCAAGGTCATcc < 1:185255/37‑1 (MQ=255) | CTGGGATGAGAGCGTGAAGATCCCGGCAGAAGAAGTCACAGTACGCTTTGAACAAGGTCATCC > minE/2144404‑2144466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |