Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	962,083	A→G	Y214H (TAT→CAT)	yddB ←	predicted porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	962,083	0	A	G	100.0%	352.5 / NA	93	Y214H (TAT→CAT)	yddB	predicted porin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (62/31);  total (62/31)

ATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGA  >  minE/962021‑962133                                                               |                                                   aTCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTc                                            >  1:266047/1‑71 (MQ=255) aTCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTc                                            >  1:4471659/1‑71 (MQ=255) aTCAGCGAGATCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTc                                            >  1:1978578/1‑71 (MQ=255)      cGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         >  1:3218376/1‑69 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGcc                                  <  1:3956903/70‑1 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCt                                 >  1:1031808/1‑71 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCt                                 >  1:37469/1‑71 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCt                                 >  1:2355082/1‑71 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCt                                 >  1:2787639/1‑71 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCt                                 >  1:1461904/1‑71 (MQ=255)            ccTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCt                                 >  1:1024821/1‑71 (MQ=255)             cTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCtt                                <  1:3374834/71‑1 (MQ=255)             cTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCtt                                <  1:332958/71‑1 (MQ=255)               gATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGa                              >  1:464472/1‑71 (MQ=255)               gATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGa                              >  1:1757118/1‑71 (MQ=255)               gATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGa                              >  1:1318936/1‑71 (MQ=255)               gATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGa                              >  1:1919683/1‑71 (MQ=255)               gATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGa                              >  1:3333148/1‑71 (MQ=255)               gATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGa                              >  1:444425/1‑71 (MQ=255)                   aaaCGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           >  1:1397416/1‑70 (MQ=255)                   aaaCGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           >  1:3942644/1‑70 (MQ=255)                   aaaCGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           >  1:4739930/1‑70 (MQ=255)                   aaaCGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           >  1:4333925/1‑70 (MQ=255)                    aaCGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTaaa                         >  1:3607456/1‑71 (MQ=255)                            ggTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCtt                                <  1:1647110/56‑1 (MQ=255)                              tataAAAGTTCTTTTTAATATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTg               <  1:2877573/71‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:3445394/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:3436961/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:2743746/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:2368091/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:3074701/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:2117791/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:1104516/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:1263736/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:2164615/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:1414340/45‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTg               <  1:62026/71‑1 (MQ=255)                              tataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCGCTTg               <  1:1810268/71‑1 (MQ=255)                               ataAAAGTTCTTTTTAAAATCTTGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:4804218/1‑71 (MQ=255)                               ataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:2477172/1‑71 (MQ=255)                               ataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:1158340/1‑71 (MQ=255)                               ataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:2006036/1‑71 (MQ=255)                               ataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:2204659/1‑71 (MQ=255)                               ataAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:3835818/1‑71 (MQ=255)                                taAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:816511/1‑70 (MQ=255)                                taAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:3209677/1‑70 (MQ=255)                                taAAAGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTCAAGAGCCTTGATTAAATGCGCTCTTGt              >  1:71785/1‑70 (MQ=255)                                  aaaGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt           >  1:2928088/1‑71 (MQ=255)                                  aaaGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt           >  1:3746105/1‑71 (MQ=255)                                  aaaGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt           >  1:227929/1‑71 (MQ=255)                                  aaaGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt           >  1:2114554/1‑71 (MQ=255)                                  aaaGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt           >  1:2658351/1‑71 (MQ=255)                                  aaaGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt           >  1:1653447/1‑71 (MQ=255)                                    aGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtc         >  1:2157134/1‑71 (MQ=255)                                    aGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtc         >  1:2867774/1‑71 (MQ=255)                                    aGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtc         >  1:2683831/1‑71 (MQ=255)                                    aGTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtc         >  1:4372618/1‑71 (MQ=255)                                     gTTCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtct        >  1:1050417/1‑71 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTg                                         <  1:656926/37‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           <  1:846934/51‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           <  1:435515/51‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           <  1:2568366/51‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa                           <  1:3094724/51‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtctc       <  1:2053129/71‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtctc       <  1:2367909/71‑1 (MQ=255)                                      ttCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtctc       <  1:1241398/71‑1 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtctc       <  1:3406056/70‑1 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:4384044/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:4879114/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:4094084/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:4254940/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:4248258/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:3099017/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:3143470/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:2813597/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:3710348/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:3718790/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:3761014/1‑71 (MQ=255)                                       tCTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCa      >  1:4086318/1‑71 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:2541579/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:1815238/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:3650329/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:1637127/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:2577682/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:156042/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:630288/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:2872087/1‑64 (MQ=255)                                        cTTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa            >  1:4246660/1‑64 (MQ=255)                                            ttAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGa  <  1:2308091/70‑1 (MQ=255)                                            ttAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGa  <  1:4454688/70‑1 (MQ=255)                                            ttAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGa  <  1:1580353/70‑1 (MQ=255)                                            ttAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGa  <  1:3289125/70‑1 (MQ=255)                                             tAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGa  <  1:4427456/69‑1 (MQ=255)                                                               |                                                   ATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTCTTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGA  >  minE/962021‑962133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑