BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	967,816	G→T	A190D (GCT→GAT)	ydeV ←	predicted sugar kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	967,816	0	G	T	100.0%	437.6 / NA	133	A190D (GCT→GAT)	ydeV	predicted sugar kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (74/59); total (74/59)

Only 100 of 133 total aligned reads displayed.

CAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGAT  >  minE/967751‑967874

cagccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTa                                                       <  1:2445215/71‑1 (MQ=255)

cagccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTa                                                       <  1:4782966/71‑1 (MQ=255)

cagccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTa                                                       <  1:1867264/71‑1 (MQ=255)

cagccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTa                                                       <  1:3930402/71‑1 (MQ=255)

   ccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTagag                                                    <  1:98202/71‑1 (MQ=255)

   ccaTATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTagag                                                    <  1:3997188/71‑1 (MQ=255)

        tCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATc                                               <  1:656631/71‑1 (MQ=255)

        tCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATc                                               <  1:3214309/71‑1 (MQ=255)

        tCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATc                                               <  1:3812682/71‑1 (MQ=255)

        tCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGAATc                                               >  1:1704233/1‑71 (MQ=255)

        tCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGAATc                                               >  1:3257857/1‑71 (MQ=255)

        tCCAGCAATGCAGGTTTCCAGTCACGGGTGGGTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATc                                               <  1:2420065/71‑1 (MQ=255)

          cAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATcc                                              <  1:391357/70‑1 (MQ=255)

          cAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATcc                                              <  1:373452/70‑1 (MQ=255)

          cAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATcc                                              <  1:3340288/70‑1 (MQ=255)

          cAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATcc                                              <  1:1788236/70‑1 (MQ=255)

          cAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATcc                                              <  1:2471911/70‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:3152472/71‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:4164048/71‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:4674613/71‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:4748918/71‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:2790890/71‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:746463/71‑1 (MQ=255)

            gCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:2294814/71‑1 (MQ=255)

             cAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAcc                                           <  1:4310249/70‑1 (MQ=255)

                  cAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAg                                      <  1:457265/70‑1 (MQ=255)

                  cAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAg                                      <  1:456984/70‑1 (MQ=255)

                  cAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAg                                      <  1:3805105/70‑1 (MQ=255)

                       ttCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTcgcc                                >  1:2280998/1‑71 (MQ=255)

                       ttCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTcgcc                                >  1:4875520/1‑71 (MQ=255)

                       ttCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTcgc                                 >  1:1952791/1‑70 (MQ=255)

                          cAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAc                                            >  1:2480966/1‑56 (MQ=255)

                          cAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAc                                            >  1:3702154/1‑56 (MQ=255)

                          cAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAc                                            >  1:2587396/1‑56 (MQ=255)

                          cAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCAc                                            >  1:1290466/1‑56 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:3693859/1‑69 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:1746430/1‑69 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:4581833/1‑69 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:3377986/1‑69 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:1775667/1‑69 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:1099923/1‑69 (MQ=255)

                            gTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCt                             >  1:3068985/1‑69 (MQ=255)

                             tCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          <  1:2364934/71‑1 (MQ=255)

                             tCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          <  1:4344463/71‑1 (MQ=255)

                             tCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          <  1:1537112/71‑1 (MQ=255)

                             tCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          <  1:2574639/71‑1 (MQ=255)

                             tCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGAATCCACCGCCAGTTCGCCGCTGAg                          <  1:4249109/71‑1 (MQ=255)

                              cACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          >  1:1036996/1‑70 (MQ=255)

                              cACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          <  1:1232340/70‑1 (MQ=255)

                              cACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          >  1:1649896/1‑70 (MQ=255)

                              cACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          >  1:2881825/1‑70 (MQ=255)

                               aCGGGTTGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:2182999/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGAt                                                >  1:3811677/1‑47 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:3175739/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:1067837/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:2141047/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:113815/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:2838184/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:2112368/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:4377416/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:2900905/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:1434446/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:3951181/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:1566131/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:2544088/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGc                                         >  1:2684270/1‑54 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCTGCTGAGCa                        >  1:4630061/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:3788448/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:4873006/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:4798979/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:1933296/71‑1 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:2825982/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:4581075/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:2450514/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:4410372/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:1000708/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:1388502/1‑71 (MQ=255)

                               aCGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        >  1:674485/1‑71 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:2744725/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:3755114/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:2629210/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:699475/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:4488922/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:4349833/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:2675442/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCa                        <  1:3472026/64‑1 (MQ=255)

                                      gtTAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGgc                 <  1:2133572/71‑1 (MQ=255)

                                       ttAGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAg                          <  1:3238951/61‑1 (MQ=255)

                                         aGATCAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATc                                               <  1:3573208/38‑1 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:549732/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:925522/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:281438/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:3340159/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:2455083/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:3565324/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:2013557/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:3078633/1‑71 (MQ=255)

                                             cAAGAAGTCCCGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGccagccag          >  1:3107162/1‑71 (MQ=255)

                                                      ccGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGAt  >  1:20198/1‑70 (MQ=255)

                                                      ccGTGGTGCCATCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGAt  >  1:352493/1‑70 (MQ=255)

CAGCCATATCCAGCAATGCAGGTTTCCAGTCACGGGTGGTTAGATCAAGAAGTCCCGTGGTGCCAGCGTTAGAGGGATCCACCGCCAGTTCGCCGCTGAGCATATAGGCCAGCCAGTCGCTGAT  >  minE/967751‑967874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`