Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,416,539	T→C	V244V (GTT→GTC)	yejM →	predicted hydrolase, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,416,539	0	T	C	100.0%	282.0 / NA	76	V244V (GTT→GTC)	yejM	predicted hydrolase, inner membrane
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/44);  total (32/44)

GATGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTG  >  minE/1416480‑1416593                                                            |                                                       gATGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGt                                              >  1:2850069/1‑70 (MQ=255) gATGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGt                                              >  1:1571592/1‑70 (MQ=255)       cAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGa                                       <  1:3972775/71‑1 (MQ=255)       cAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGa                                       <  1:3967165/71‑1 (MQ=255)       cAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGa                                       <  1:225202/71‑1 (MQ=255)       cAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGa                                       <  1:3440672/71‑1 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGaa                                      >  1:1124322/1‑70 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:1415695/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:3011229/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:4877976/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:1827019/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:1865062/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:3370825/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:3985782/1‑71 (MQ=255)         ggAGTATCAACGCCGTCTTATTGAGCAAAGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:855886/1‑71 (MQ=255)         gcaGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAAc                                     >  1:4082828/3‑71 (MQ=255)               tCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTgcgc                                >  1:3714498/1‑70 (MQ=255)               tCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTgcgc                                >  1:3196260/1‑70 (MQ=255)               tCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCt                               <  1:4884308/71‑1 (MQ=255)               tCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTGCCGTCCAGTATCCGTTAAGCGAACTgcgc                                >  1:339867/1‑70 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:885436/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:2849794/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:281625/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:2756752/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:255665/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:2458843/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:2199817/1‑71 (MQ=255)                 aaCGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTAt                             >  1:1779220/1‑71 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:4261830/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:4667049/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:4758055/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:4778705/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:606364/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:715653/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:869786/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:98337/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:2904083/71‑1 (MQ=255)                      cGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:1252737/71‑1 (MQ=255)                       gTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:4480897/70‑1 (MQ=255)                       gTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGa                        <  1:4032367/70‑1 (MQ=255)                                aGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCAc               <  1:2346477/70‑1 (MQ=255)                                aGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCAc               <  1:4535803/70‑1 (MQ=255)                                aGCAAGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCAc               <  1:1107979/70‑1 (MQ=255)                                   aaGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCggg           <  1:3008556/71‑1 (MQ=255)                                   aaGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCggg           <  1:818312/71‑1 (MQ=255)                                    aGGTAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCggg           <  1:2634231/70‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:3143673/71‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:624971/71‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:1400546/71‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:4407258/71‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:4097433/71‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:2578706/71‑1 (MQ=255)                                       tAATCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:3100963/71‑1 (MQ=255)                                        aaTCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:4235630/70‑1 (MQ=255)                                        aaTCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:109879/70‑1 (MQ=255)                                         aTCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACAGCGCTATCGCGATATGGGCACCGGGCAGAAt     >  1:2010742/1‑71 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGatat                     >  1:566542/1‑54 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATg                    >  1:8141/1‑55 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATg                    >  1:874786/1‑55 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATg                    >  1:1043473/1‑55 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATg                    >  1:1459717/1‑55 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATg                    >  1:1726303/1‑55 (MQ=255)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGCAAACTGCGCTATCGCGATATg                    >  1:3793671/1‑55 (MQ=39)                                          tCCAGACGCCGTTTCCGTCCAGTATCCGTTAAGAGAACTGCGCTATCGCGATATg                    >  1:4076407/1‑55 (MQ=39)                                            cAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTTCGCTATCGCGATATGGGCACCGGGCAGAAtgtg  <  1:3265033/71‑1 (MQ=255)                                            cAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAAtgtg  <  1:1448189/71‑1 (MQ=255)                                            cAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAAtgtg  <  1:2722349/71‑1 (MQ=255)                                            cAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAAtgtg  <  1:2880013/71‑1 (MQ=255)                                            cAGACGCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAAtgtg  <  1:949327/71‑1 (MQ=255)                                                 gCCGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAAtgtg  <  1:1604417/66‑1 (MQ=255)                                                  ccGTTTCCGTCCTGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:75167/60‑1 (MQ=39)                                                  ccGTTTCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGa       <  1:4412978/60‑1 (MQ=255)                                                      ttCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAg        <  1:4647823/55‑1 (MQ=255)                                                      ttCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAg        <  1:4527250/55‑1 (MQ=255)                                                      ttCCGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAg        <  1:257742/55‑1 (MQ=255)                                                        ccGTCCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAg        <  1:2883522/53‑1 (MQ=255)                                                            |                                                       GATGCGCAGGAGTATCAACGCCGTCTTATTGAGCAAGGTAATCCAGACGCCGTTTCCGTTCAGTATCCGTTAAGCGAACTGCGCTATCGCGATATGGGCACCGGGCAGAATGTG  >  minE/1416480‑1416593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑