Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,568,107	C→T	G407R (GGG→AGG)	mntH ←	manganese/divalent cation transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,568,107	0	C	T	100.0%	249.4 / NA	77	G407R (GGG→AGG)	mntH	manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/40);  total (37/40)

TTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCG  >  minE/1568042‑1568173                                                                  |                                                                   tttACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCtcacc                                                                >  1:3821276/1‑70 (MQ=255)   tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACcaa                                                              >  1:3533359/1‑70 (MQ=255)   tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACcaa                                                              >  1:4018791/1‑70 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:108629/71‑1 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:4187599/71‑1 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:638360/71‑1 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:3990574/71‑1 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:2434529/71‑1 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:695857/71‑1 (MQ=255)          cATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:1891806/71‑1 (MQ=255)           aTCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa                                                      <  1:357269/70‑1 (MQ=255)              cGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGata                                                    >  1:4058035/1‑69 (MQ=255)              cGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATAtt                                                  >  1:3278999/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCa                                                >  1:1966983/1‑70 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:2909414/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:4695704/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               <  1:3750263/71‑1 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:2768170/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               <  1:4167937/71‑1 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:4511152/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               <  1:4383546/71‑1 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:1612085/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:1572965/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:1521468/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:1512501/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:900165/1‑71 (MQ=255)                 cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAg                                               >  1:1138353/1‑71 (MQ=255)                      gTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGcc                                           <  1:2091674/70‑1 (MQ=255)                      gTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGcc                                           <  1:19991/70‑1 (MQ=255)                         ccAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGa                                       >  1:3362926/1‑71 (MQ=255)                            gATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACcaac                                                             >  1:34762/1‑46 (MQ=255)                            gATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGAc                                      >  1:664119/1‑69 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCa                                          >  1:413396/1‑62 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGca                                 >  1:3141025/1‑71 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGca                                 >  1:4309816/1‑71 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGca                                 >  1:2561706/1‑71 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGca                                 >  1:4916975/1‑71 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGca                                 >  1:1983387/1‑71 (MQ=255)                               gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGca                                 >  1:4088601/1‑71 (MQ=255)                                cGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                >  1:4564969/1‑71 (MQ=255)                                 gACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                               <  1:3106294/71‑1 (MQ=255)                                 gACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                               <  1:3066897/71‑1 (MQ=255)                                 gACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                               <  1:4457000/71‑1 (MQ=255)                                 gACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                               <  1:713610/71‑1 (MQ=255)                                    gCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcaccac                             >  1:3069418/1‑70 (MQ=255)                                    gCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGcaccac                             >  1:1093763/1‑70 (MQ=255)                                      tCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAAt                          >  1:1615756/1‑71 (MQ=255)                                                aCAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCct                >  1:2675390/1‑71 (MQ=255)                                                      ccAGCTCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          >  1:4590514/1‑71 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  >  1:427877/1‑63 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          >  1:4392553/1‑71 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          >  1:989203/1‑71 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          >  1:55234/1‑71 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          <  1:4139397/71‑1 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          >  1:3981479/1‑71 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          >  1:1912315/1‑71 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          <  1:2788140/71‑1 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          <  1:2825468/71‑1 (MQ=255)                                                      ccAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt          <  1:2891530/71‑1 (MQ=255)                                                           gcCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAc     <  1:228561/71‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:3284724/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:1286081/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:1403068/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:1439528/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:1613698/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:405133/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:2147498/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:2223292/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:2512522/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:3415308/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:2870196/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:4279185/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:308770/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                  <  1:3407610/55‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAcgcg  <  1:4709980/71‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAcgcg  <  1:3785319/71‑1 (MQ=255)                                                              gTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAcgcg  <  1:621035/71‑1 (MQ=255)                                                               tCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCATCCAGc                  <  1:4513892/54‑1 (MQ=39)                                                                  |                                                                   TTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCG  >  minE/1568042‑1568173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑