Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,919,248	G→A	Q205Q (CAG→CAA)	tas →	predicted oxidoreductase, NADP(H)‑dependent aldo‑keto reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,919,248	0	G	A	100.0%	293.1 / NA	90	Q205Q (CAG→CAA)	tas	predicted oxidoreductase, NADP(H)‑dependent aldo‑keto reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/38);  total (52/38)

GGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGG  >  minE/1919186‑1919315                                                               |                                                                    ggCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCtt                                                              <  1:2288217/70‑1 (MQ=255) ggCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCtt                                                              <  1:2248080/70‑1 (MQ=255) ggCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCtt                                                              <  1:3870150/70‑1 (MQ=255) ggCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCtt                                                              <  1:3691654/70‑1 (MQ=255) ggCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCtt                                                              <  1:2794867/70‑1 (MQ=255)  gCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTa                                                             <  1:1636732/70‑1 (MQ=255)         gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:3109423/1‑71 (MQ=255)         gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:592865/1‑71 (MQ=255)         gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:2165065/1‑71 (MQ=255)         gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:2664701/1‑71 (MQ=255)         gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:1807956/1‑71 (MQ=255)         gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:2980979/1‑71 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:3692611/1‑70 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:4124369/1‑70 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:3945310/1‑70 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:1963994/1‑70 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:3671045/1‑70 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:3365904/1‑70 (MQ=255)          cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt                                                     >  1:704839/1‑70 (MQ=255)           gcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:4302678/71‑1 (MQ=255)           gcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:3786506/71‑1 (MQ=255)           gcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:4759881/71‑1 (MQ=255)           gcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:1917426/71‑1 (MQ=255)           gcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:4249579/71‑1 (MQ=255)           gcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:1380927/71‑1 (MQ=255)                 aggaTCTGGCGGAAAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTa                                                   <  1:1328602/62‑1 (MQ=255)                  gCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAACACCCTTACAGt                                                         >  1:3664788/1‑58 (MQ=39)                  gCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCg                                              >  1:44891/1‑69 (MQ=255)                  gCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCg                                              >  1:4783558/1‑69 (MQ=255)                  gCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCg                                              >  1:2568666/1‑69 (MQ=255)                  gCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCg                                              >  1:326271/1‑69 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGtt                                                    >  1:2007873/1‑57 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:4205922/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:101401/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:1354505/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:1467230/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:3027142/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:4497657/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:1645258/1‑70 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGATAAACCGCAGtt                                         >  1:2664516/1‑68 (MQ=255)                        ggCGGACAAACACGATCTGCCGCGTAATGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGtttt                                       >  1:2894206/1‑70 (MQ=255)                            gACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGt                                  <  1:4451297/71‑1 (MQ=255)                            gACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGt                                  <  1:2422625/71‑1 (MQ=255)                                    cGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGttt                                        >  1:1821064/1‑57 (MQ=255)                                    cGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGttt                                        >  1:4733127/1‑57 (MQ=255)                                    cGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGttt                                        >  1:4225725/1‑57 (MQ=255)                                           ccGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt                   <  1:3783317/71‑1 (MQ=255)                                           ccGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt                   <  1:4275245/71‑1 (MQ=255)                                           ccGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt                   <  1:2950225/71‑1 (MQ=255)                                           ccGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt                   <  1:1185928/71‑1 (MQ=255)                                           ccGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGGCTGGCAGAAGt                   <  1:1452603/71‑1 (MQ=255)                                                tATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg                       <  1:971556/62‑1 (MQ=255)                                                tATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg                       <  1:1506066/62‑1 (MQ=255)                                                tATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg                       <  1:1714353/62‑1 (MQ=255)                                                tATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg                       <  1:1196093/62‑1 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:2260462/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:4700314/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:4669145/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:1597794/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:1483772/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:3244016/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:1935667/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:4143294/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:1123545/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:2510034/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:931873/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:2669692/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:2829038/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:3033038/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa                                    >  1:956654/1‑45 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTa          >  1:3902379/1‑71 (MQ=255)                                                    gTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTa          >  1:3033695/1‑71 (MQ=255)                                                     tCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTa          <  1:2922165/70‑1 (MQ=255)                                                     tCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTa          <  1:1576027/70‑1 (MQ=255)                                                       aCCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATg        <  1:2769062/70‑1 (MQ=255)                                                       aCCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATg        <  1:3329327/70‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:1779488/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:632085/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:4829354/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:1073796/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:4585327/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:1639182/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:174667/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:4275880/36‑1 (MQ=255)                                                           ttCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:3062623/36‑1 (MQ=255)                                                            tCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTg                                      <  1:2641417/35‑1 (MQ=255)                                                            tCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAg            >  1:1990103/1‑61 (MQ=255)                                                            tCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAgggg  >  1:2060185/1‑71 (MQ=255)                                                            tCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAgggg  >  1:4115706/1‑71 (MQ=255)                                                            tCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAgggg  >  1:3292104/1‑71 (MQ=255)                                                               |                                                                    GGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGG  >  minE/1919186‑1919315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑