Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,039,506 C→T V137I (GTT→ATT)  ribB ← 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,039,5060CT100.0% 414.1 / NA 126V137I (GTT→ATT) ribB3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/90);  total (36/90)

Only 100 of 126 total aligned reads displayed.

ATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAA  >  minE/2039438‑2039571
                                                                    |                                                                 
aTCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGt                                                                 <  1:3941102/71‑1 (MQ=255)
aTCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGt                                                                 >  1:1129706/1‑71 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:4729029/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:3969425/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:844216/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:3693773/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:2949218/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:335857/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:567409/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:4332696/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:2247542/1‑70 (MQ=255)
          gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGAc                                                        >  1:390210/1‑70 (MQ=255)
              cAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGAtt                                                    >  1:1080568/1‑70 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:2378077/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:170038/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:821916/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:2295491/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:4357650/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:315776/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:1450952/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:3896058/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:2556954/71‑1 (MQ=255)
                gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg                                                 <  1:1181840/71‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:294940/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:2539550/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:4151199/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:507344/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:1543336/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:381446/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:885891/70‑1 (MQ=255)
                     aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt                                             <  1:1197523/70‑1 (MQ=255)
                            cgcgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGttt                                     <  1:3558889/71‑1 (MQ=255)
                            cgcgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGttt                                     <  1:2459485/71‑1 (MQ=255)
                            cgcgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGttt                                     <  1:2832667/71‑1 (MQ=255)
                            cgcgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGttt                                     <  1:443611/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:4652608/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:4072488/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:3963905/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:2870912/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:3840227/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:2484817/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:784978/71‑1 (MQ=255)
                                  aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGcc                               <  1:2322174/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:2813433/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:1226199/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:1541284/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:1576838/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:3724640/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:4055385/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:3629512/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:800711/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:3473903/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:4476024/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:4421190/71‑1 (MQ=255)
                                   gTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCa                              <  1:2435397/71‑1 (MQ=255)
                                      caccacCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCg                           >  1:4689152/1‑71 (MQ=255)
                                          accTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCaa                       >  1:3302676/1‑71 (MQ=255)
                                          accTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCaa                       >  1:1371555/1‑71 (MQ=255)
                                          accTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCaa                       >  1:1033238/1‑71 (MQ=255)
                                             tgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg                    >  1:308625/1‑71 (MQ=255)
                                             tgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg                    >  1:1785264/1‑71 (MQ=255)
                                             tgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg                    >  1:2494799/1‑71 (MQ=255)
                                             tgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg                    >  1:2338967/1‑71 (MQ=255)
                                             tgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg                    >  1:1359894/1‑71 (MQ=255)
                                             tgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg                    >  1:2761230/1‑71 (MQ=255)
                                               cctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTg                                            >  1:4397372/1‑45 (MQ=255)
                                               cctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCt                  >  1:1683633/1‑71 (MQ=255)
                                               cctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCt                  >  1:1118988/1‑71 (MQ=255)
                                               cctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCt                  >  1:3906471/1‑71 (MQ=255)
                                                    gcgcGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCAt                             >  1:913269/1‑55 (MQ=255)
                                                    gcgcGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCAt                             >  1:1945600/1‑55 (MQ=255)
                                                    gcgcGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGcgcg             <  1:416097/71‑1 (MQ=255)
                                                    gcgcGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGcgcg             <  1:205644/71‑1 (MQ=255)
                                                    gcgcGAAGTGGGAAAATGTGGCCAGGACGATACAGATCTGACGGTATTGCGCCAt                             >  1:370648/1‑55 (MQ=255)
                                                     cgcgAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGcgcg             <  1:1560787/70‑1 (MQ=255)
                                                     cgcgAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGcgcg             <  1:3364147/70‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:3779697/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:106087/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:709920/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:4897141/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:2591047/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:2687835/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGt       <  1:2932937/71‑1 (MQ=255)
                                                          aGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGAGCGAACGGt       <  1:3705997/71‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:1503741/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:4600408/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:2530357/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:4040647/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:1683974/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:2109231/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:588512/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:3626671/70‑1 (MQ=255)
                                                              ggAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:2048374/70‑1 (MQ=255)
                                                               gAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt    <  1:4212659/69‑1 (MQ=255)
                                                               gAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa  <  1:3816068/71‑1 (MQ=255)
                                                               gAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa  <  1:4353856/71‑1 (MQ=255)
                                                               gAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa  <  1:2406667/71‑1 (MQ=255)
                                                               gAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa  <  1:3327791/71‑1 (MQ=255)
                                                               gAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa  <  1:3039505/71‑1 (MQ=255)
                                                                aaaaTGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa  <  1:2383788/70‑1 (MQ=255)
                                                                    |                                                                 
ATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAA  >  minE/2039438‑2039571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑