Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,042,482	C→T	M57I (ATG→ATA)	glgS ←	predicted glycogen synthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,042,482	0	C	T	100.0%	184.3 / NA	57	M57I (ATG→ATA)	glgS	predicted glycogen synthesis protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/33);  total (24/33)

CAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGT  >  minE/2042419‑2042546                                                                |                                                                 cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTatcatc                                                            <  1:131336/70‑1 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTatcatc                                                            <  1:3824809/70‑1 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTatcatc                                                            <  1:4230698/70‑1 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTatcatc                                                            <  1:4626538/70‑1 (MQ=255)     cacTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGtt                                                        >  1:4911698/1‑70 (MQ=255)      acTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGa                                                      <  1:1012647/71‑1 (MQ=255)      acTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGa                                                      <  1:335586/71‑1 (MQ=255)      acTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGa                                                      <  1:3518313/71‑1 (MQ=255)      acTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGa                                                      <  1:3837265/71‑1 (MQ=255)                        gCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtat                                                                >  1:778737/1‑43 (MQ=255)                        gCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtat                                                                >  1:3688084/1‑43 (MQ=255)                        gCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtat                                                                >  1:1516692/1‑43 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCTAGCTCTCTTGCCTGtatcat                                                             <  1:2317692/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4034036/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:2764044/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:2854911/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3905278/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3875743/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:711875/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3801493/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3773975/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3053951/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3328928/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3744444/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3162374/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3577004/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:1689186/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:2501578/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4154218/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4160395/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:420771/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4209828/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:1571859/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4298662/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:440894/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:2056109/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:2055456/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4454767/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4856748/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4769176/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:1811671/45‑1 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:1232877/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:3747181/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:4130490/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:2377633/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:248721/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:2865501/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:3126569/1‑55 (MQ=255)                         cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa                                                   >  1:3081334/1‑55 (MQ=255)                         cccGTTATATCACTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:4719483/45‑1 (MQ=38)                          ccGTTATATGAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:3306219/44‑1 (MQ=38)                          ccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:1596992/44‑1 (MQ=255)                           cGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatcat                                                             <  1:416057/43‑1 (MQ=255)                               atatCAGTGCTCTAACTGCAGCTATCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa                              <  1:4061823/70‑1 (MQ=255)                               atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGTaa                              <  1:555807/70‑1 (MQ=255)                               atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa                              <  1:3024399/70‑1 (MQ=255)                               atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa                              <  1:4871358/70‑1 (MQ=255)                               atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa                              <  1:2246174/70‑1 (MQ=255)                               atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGGGCGCaa                              <  1:2295/70‑1 (MQ=255)                                 atCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAc                           >  1:2297578/1‑71 (MQ=255)                                 atCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAc                           >  1:2791615/1‑71 (MQ=255)                                 atCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAc                           >  1:922478/1‑71 (MQ=255)                                  tCAGTGCTCTAACTCCAGCTCTCTTGCCTGTatcatc                                                            <  1:837587/37‑1 (MQ=255)                                  tCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAcc                          <  1:1447802/71‑1 (MQ=255)                                  tCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAcc                          <  1:3566835/71‑1 (MQ=255)                                  tCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAcc                          <  1:3864233/71‑1 (MQ=255)                                  tCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAcc                          <  1:382251/71‑1 (MQ=255)                                    aGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAAt                                                  >  1:2182459/1‑45 (MQ=255)                                    aGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATa                                                 >  1:2047825/1‑46 (MQ=255)                                    aGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATa                                                 >  1:2938660/1‑46 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:2359699/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:597473/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:3798463/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:2793798/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:48211/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:1823399/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:470802/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:796507/69‑1 (MQ=255)                                           tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt                   <  1:2878923/69‑1 (MQ=255)                                             aCTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCTAAACCAGGTTCTATGt               >  1:1293070/1‑71 (MQ=255)                                             aCTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGt               >  1:4814288/1‑71 (MQ=255)                                             aCTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGt               >  1:738412/1‑71 (MQ=255)                                                      ctctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATc       >  1:3230490/1‑70 (MQ=255)                                                      ctctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATc       >  1:2968758/1‑70 (MQ=255)                                                       tctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATcc      >  1:4279470/1‑70 (MQ=255)                                                       tctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCa     >  1:1653580/1‑71 (MQ=255)                                                       tctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCa     >  1:3111964/1‑71 (MQ=255)                                                       tctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCa     >  1:2889582/1‑71 (MQ=255)                                                           gCCTGTATCATCTGTTGACAATACCAGGCATAACGTGGGCAAAACCAGGTTCTATGTTCTTCATCCATGt  <  1:3617414/70‑1 (MQ=255)                                                           gCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt  <  1:68582/70‑1 (MQ=255)                                                           gCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt  <  1:1880804/70‑1 (MQ=255)                                                           gCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt  <  1:94705/70‑1 (MQ=255)                                                            ccTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt  <  1:88825/69‑1 (MQ=255)                                                                |                                                                 CAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGT  >  minE/2042419‑2042546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑