| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,493,363 | G→A | E720E (GAG→GAA) | gyrB → | DNA gyrase, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,363 | 0 | G | A | 100.0% | 50.9 / NA | 17 | E720E (GAG→GAA) | gyrB | DNA gyrase, subunit B |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/7); total (10/7) | |||||||||||
CGTGGCTTGCTGGAAGAAGATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAGCAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCCTCTCCATCCAGCGTTATAAAGGTCTG > minE/2493295‑2493429 | cGTGGCTTGCTGGAAGAAGATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACa < 1:2832429/71‑1 (MQ=255)cGTGGCTTGCTGGAAGAAGATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACa < 1:3239853/71‑1 (MQ=255) aagaagATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGActgg > 1:1275209/1‑71 (MQ=255) aagaagATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGActgg > 1:1609534/1‑71 (MQ=255) aagaagATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGActgg > 1:360567/1‑71 (MQ=255) aagaagATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGActgg > 1:4502530/1‑71 (MQ=255) cGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGACTGGCTGGTGaaa < 1:1186478/71‑1 (MQ=255) cGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGACTGGCTGGTGaaa < 1:953397/71‑1 (MQ=255) aTCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCTAACAGGCGCTGGACTGGCTGGTGAAAGAGt < 1:670094/70‑1 (MQ=255) gCGAGCGTCGTCAGCCGGTAGCCGGCTTCGAACAGGCGCTGGACTGGCTGGTGAAaga > 1:3843175/1‑58 (MQ=39) gCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGACTGGCTGGTGAAaga > 1:678102/1‑58 (MQ=255) gCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGACTGGCTGGTGAAaga > 1:1124290/1‑58 (MQ=255) gAGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGACTGGCTGGTGaaa < 1:3445356/54‑1 (MQ=255) aGCGTCGTCAGCCGGTAGCCAGCTTCGAACAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCctc < 1:1630992/71‑1 (MQ=255) tCGAACAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCCTCTCCATCCAGCGTTATAAAGGTCTg > 1:4040806/1‑71 (MQ=255) tCGAACAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCCTCTCCATCCAGCGTTATAAAGGTCTg > 1:2752645/1‑71 (MQ=255) tCGAACAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCCTCTCCATCCAGCGTTATAAAGGTCTg > 1:996137/1‑71 (MQ=255) | CGTGGCTTGCTGGAAGAAGATGCGTTTATCGAACGTGGCGAGCGTCGTCAGCCGGTAGCCAGCTTCGAGCAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCCTCTCCATCCAGCGTTATAAAGGTCTG > minE/2493295‑2493429 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |