Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	298,431	G→A	M364I (ATG→ATA)	mdlA →	fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	298,431	0	G	A	100.0%	150.1 / NA	47	M364I (ATG→ATA)	mdlA	fused predicted multidrug transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/13);  total (34/13)

TTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTT  >  minE/298363‑298496                                                                     |                                                                  ttCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACt                                                                 <  1:93556/71‑1 (MQ=255) ttCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACt                                                                 <  1:1961760/71‑1 (MQ=255)              aGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCggg                                                    <  1:3683520/71‑1 (MQ=255)                 cTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGcc                                                  >  1:1079580/1‑70 (MQ=255)                 cTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGcc                                                  >  1:903759/1‑70 (MQ=255)                 cTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGcc                                                  >  1:1312781/1‑70 (MQ=255)                 cTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGcc                                                  >  1:869861/1‑70 (MQ=255)                 cTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGcc                                                  >  1:2255228/1‑70 (MQ=255)                          cTGCGCTGGAAAATGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:369918/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:3044543/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:2677139/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:4165508/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:4361901/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:4815276/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:831417/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:1522145/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTcc                                        >  1:1099962/1‑71 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTc                                         >  1:199547/1‑70 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTc                                         >  1:3499487/1‑70 (MQ=255)                          cTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGATCAGATACTGGGTATCTGCGGGCCGACTGGTTc                                         >  1:3642746/1‑70 (MQ=255)                          cTGCCCAGGAAAACGTCAAATTCGCCCTGAACCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGATc                                         >  1:155/1‑70 (MQ=255)                                 ggAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCaa                                   >  1:1079926/1‑69 (MQ=255)                                 ggAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCaa                                   >  1:1934567/1‑69 (MQ=255)                                 ggAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCaa                                   >  1:2126096/1‑69 (MQ=255)                                      aCGTCAATTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTc                                         >  1:311080/1‑58 (MQ=255)                                           aaTTTCGCCCTGAAACCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCtgtt                       <  1:3021647/71‑1 (MQ=255)                                                       aaaCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCAtt             <  1:4776872/69‑1 (MQ=255)                                                         aCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAg          >  1:418292/1‑70 (MQ=255)                                                         aCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc         >  1:2490632/1‑71 (MQ=255)                                                         aCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc         >  1:3816947/1‑71 (MQ=255)                                                         aCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc         >  1:539923/1‑71 (MQ=255)                                                         aCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc         >  1:2446492/1‑71 (MQ=255)                                                         aCCCGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGc         >  1:2414870/1‑71 (MQ=255)                                                            cgttCAGATACTGGGTATCTGCGGGCCGACTTGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:391576/68‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:1111590/71‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:1271248/71‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:816560/71‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:582536/71‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:3971245/71‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:3860615/71‑1 (MQ=255)                                                            cGGTCAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTc      <  1:2458640/71‑1 (MQ=255)                                                                cAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCGTTCAGCGTCAttt  >  1:4593450/1‑71 (MQ=255)                                                                cAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAttt  >  1:4195662/1‑71 (MQ=255)                                                                cAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAttt  >  1:3703342/1‑71 (MQ=255)                                                                cAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAttt  >  1:2025205/1‑71 (MQ=255)                                                                cAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAttt  >  1:2929237/1‑71 (MQ=255)                                                                cAGATACTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCAttt  >  1:345624/1‑71 (MQ=255)                                                                     |                                                                  TTCACGTATCCGCAGACTGACCATCCTGCGCTGGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTT  >  minE/298363‑298496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑