Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 458,679 G→A G73S (GGC→AGC)  nei → endonuclease VIII

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE458,6790GA100.0% 367.1 / NA 112G73S (GGC→AGC) neiendonuclease VIII
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (73/39);  total (73/39)

Only 100 of 112 total aligned reads displayed.

GCGTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACGGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTAAAA  >  minE/458612‑458741
                                                                   |                                                              
gcgTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCg                                                             >  1:421151/1‑71 (MQ=255)
gcgTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCg                                                             >  1:2398082/1‑71 (MQ=255)
gcgTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCg                                                             >  1:2154484/1‑71 (MQ=255)
gcgTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCg                                                             >  1:4338333/1‑71 (MQ=255)
gcgTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCg                                                             >  1:3019488/1‑71 (MQ=255)
gcgTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCg                                                             >  1:1173111/1‑71 (MQ=255)
        aGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGgcgc                                                     >  1:4065321/1‑71 (MQ=255)
         ggCGTTGTTAACTCATTTTTCCAACGACTTAACG‑‑CTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTg                                                  <  1:3757783/71‑1 (MQ=255)
           cGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTg                                                  <  1:3600273/71‑1 (MQ=255)
           cGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTg                                                  <  1:3233736/71‑1 (MQ=255)
               gttAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:4162436/70‑1 (MQ=255)
                  aaCTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGAt                                            >  1:1315890/1‑70 (MQ=255)
                  aaCTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATa                                           >  1:402648/1‑71 (MQ=255)
                    cTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATAcc                                         <  1:4911397/71‑1 (MQ=255)
                    cTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATAcc                                         <  1:4608074/71‑1 (MQ=255)
                    cTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATAcc                                         <  1:1753983/71‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:1175089/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:2158516/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:2315922/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:3345013/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:1408285/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:1141401/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:137601/61‑1 (MQ=255)
                        tttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:4718984/61‑1 (MQ=255)
                         ttttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:336683/60‑1 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:1086066/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:3187998/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:2805215/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:2838966/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:3831407/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:3891957/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:4424928/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:4820040/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:494981/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:2462233/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:1315504/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:2641469/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTgg                                                 >  1:1383440/1‑57 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCg                                     >  1:1976737/1‑69 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCg                                     >  1:4038926/1‑69 (MQ=255)
                          tttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCg                                     >  1:2856747/1‑69 (MQ=255)
                           ttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGaa                                   <  1:2868078/70‑1 (MQ=255)
                           ttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGaa                                   <  1:941810/70‑1 (MQ=255)
                           ttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGaa                                   <  1:67758/70‑1 (MQ=255)
                           ttCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGaa                                   <  1:1426167/70‑1 (MQ=255)
                                 cGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCg                             >  1:4280682/1‑70 (MQ=255)
                                 cGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCg                             >  1:4866270/1‑70 (MQ=255)
                                 cGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCg                             >  1:4688845/1‑70 (MQ=255)
                                 cGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCg                             >  1:3255668/1‑70 (MQ=255)
                                 cGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCg                             >  1:2366023/1‑70 (MQ=255)
                                 cGACTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCg                             >  1:1055503/1‑70 (MQ=255)
                                   aCTTAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGtt                                               <  1:947056/50‑1 (MQ=255)
                                      tAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGa                         <  1:748458/69‑1 (MQ=255)
                                      tAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGa                         <  1:776814/69‑1 (MQ=255)
                                      tAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGa                         <  1:4781433/69‑1 (MQ=255)
                                      tAACGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGa                         <  1:3741938/69‑1 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCg                                        >  1:3390026/1‑52 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:4046271/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:2766577/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:1385068/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:1810384/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:441256/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:3884011/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:1889083/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:3823589/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:3726745/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:4778044/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:1282167/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:3499409/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:590052/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:2427268/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:3233299/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:2677899/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGAcc                       >  1:26976/1‑69 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCAc                     >  1:1753911/1‑71 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCAc                     >  1:2905390/1‑71 (MQ=255)
                                        aCGCTCTACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCAc                     >  1:1412739/1‑71 (MQ=255)
                                              tACAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAg                >  1:3974957/1‑70 (MQ=255)
                                               aCAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGa                         <  1:84653/60‑1 (MQ=255)
                                                cAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAg                <  1:1390616/68‑1 (MQ=255)
                                                cAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAg                <  1:1139038/68‑1 (MQ=255)
                                                cAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTAt             <  1:2998815/71‑1 (MQ=255)
                                                cAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTAt             <  1:2057635/71‑1 (MQ=255)
                                                cAGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTAt             <  1:3547599/71‑1 (MQ=255)
                                                 aGCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTAt             <  1:418779/70‑1 (MQ=255)
                                                  gCCATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTg           >  1:3165604/1‑71 (MQ=255)
                                                    cATAATCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCg         <  1:1524130/71‑1 (MQ=255)
                                                        aTCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGt      >  1:2795883/1‑70 (MQ=255)
                                                         tCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    <  1:3366300/71‑1 (MQ=255)
                                                         tCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    <  1:284581/71‑1 (MQ=255)
                                                         tCAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    <  1:4299614/71‑1 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:3491152/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:359961/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:2300997/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:3222824/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:723604/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:3902982/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:4571141/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:820953/1‑70 (MQ=255)
                                                          cAGCTTTACAGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTaa    >  1:4565349/1‑70 (MQ=255)
                                                                   |                                                              
GCGTGGTAAGGCGTTGTTAACTCATTTTTCCAACGACTTAACGCTCTACAGCCATAATCAGCTTTACGGCGTCTGGCGCGTGGTTGATACCGGCGAAGAGCCGCAGACCACGCGAGTATTGCGGGTAAAA  >  minE/458612‑458741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑