Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,763,556 T→C 40.3% noncoding (288/1542 nt) rrsG ← 16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,763,5560TC40.3% 31.4 / 68.0 57noncoding (288/1542 nt)rrsG16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (15/19);  new base C (16/7);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGC  >  minE/1763502‑1763624
                                                      |                                                                    
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCtt                                                       <  1:1665936/70‑1 (MQ=31)
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCtt                                                       <  1:400527/70‑1 (MQ=14)
ggAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCtt                                                       <  1:1812578/70‑1 (MQ=14)
  aGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGt                                                    >  1:19134/1‑71 (MQ=21)
    tCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGa                                                  <  1:451838/71‑1 (MQ=34)
    tCTGGACCGTGTCTCAGTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTGa                                                  <  1:393430/71‑1 (MQ=11)
     cTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAg                                                 >  1:130010/1‑71 (MQ=34)
         acagtgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                             >  1:1497165/4‑71 (MQ=21)
          ccGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGt                                             >  1:145556/1‑70 (MQ=35)
           cGTGTCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGcctt                                             <  1:1139543/69‑3 (MQ=11)
             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcc                                         >  1:1250149/1‑71 (MQ=34)
             tgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAc                                          <  1:1629028/70‑1 (MQ=35)
              gtCACAGTTCCAGTGTGGCTGGTCATCCTGTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTAccc                                        >  1:1935637/1‑71 (MQ=11)
               tctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAccc                                        >  1:1764118/1‑70 (MQ=35)
                ctcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCcca                                      >  1:284009/1‑71 (MQ=34)
                 tcAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccac                                     >  1:797423/1‑71 (MQ=21)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacc                                    >  1:1671498/1‑70 (MQ=35)
                   aGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCccacc                                    >  1:2135057/1‑70 (MQ=35)
                    gtcccAATGTGGCTGATCATCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCAtt                                            <  1:1218584/58‑1 (MQ=11)
                     ttCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCTACTGATCGTCGCCTTGGTGAGCCTTTACCTCACcaac                                 >  1:373570/1‑71 (MQ=14)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTgg                                                     <  1:580298/51‑1 (MQ=35)
                     ttCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                 <  1:604336/71‑1 (MQ=39)
                      tCCAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                 >  1:559577/1‑70 (MQ=255)
                       ccAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                               >  1:326904/1‑55 (MQ=255)
                       ccAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                 >  1:616620/1‑69 (MQ=255)
                       ccAGTGTGGCTGGTCATCCTCTCAGACCAGCCAGGCATCGTCGCCTTGGTGAGCCGTTAccc                                        >  1:1313875/1‑62 (MQ=255)
                          gtgtGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGt                                                    >  1:1134826/1‑47 (MQ=255)
                          gtgtGGCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCt                            <  1:1450601/71‑1 (MQ=255)
                               gCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                          >  1:618990/1‑68 (MQ=255)
                               gCTGGTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATc                        <  1:148132/70‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTATGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   <  1:2003371/71‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCa                     <  1:1107812/69‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCAt                    <  1:1915691/70‑1 (MQ=255)
                                   gTCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAg                                                 <  1:2053106/41‑1 (MQ=255)
                                    tCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCt                  <  1:447726/71‑1 (MQ=255)
                                    tCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAg                                                 <  1:920412/40‑1 (MQ=255)
                                    tCATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCTTCt                  >  1:1770593/1‑71 (MQ=255)
                                     cATCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGcc                                               >  1:647133/1‑41 (MQ=255)
                                      aTCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcccc                                       >  1:924616/1‑48 (MQ=38)
                                      aTCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAcccc                                       >  1:548777/1‑48 (MQ=38)
                                      aTCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                          >  1:307187/1‑61 (MQ=255)
                                      aTCCTCTCAGACAAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                          >  1:1820755/1‑61 (MQ=255)
                                       tCCTCTCAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTgg                >  1:1463060/1‑70 (MQ=255)
                                        ccTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTAc                                          <  1:834535/43‑1 (MQ=38)
                                           ctcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATc                        <  1:329499/58‑1 (MQ=255)
                                            tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                <  1:1647221/49‑1 (MQ=255)
                                            tcAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaaca                                <  1:1343490/49‑1 (MQ=255)
                                            tcAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAg                              >  1:890083/1‑51 (MQ=255)
                                             cAGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATc         >  1:1419288/1‑71 (MQ=255)
                                              aGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATcc        >  1:946237/1‑71 (MQ=255)
                                                aCCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACcaac                                 <  1:242493/44‑1 (MQ=255)
                                                aCCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGa      >  1:54856/1‑71 (MQ=255)
                                                  cAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCccacca                                   >  1:1342052/1‑40 (MQ=255)
                                                  cAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                          >  1:1443972/1‑49 (MQ=255)
                                                   aGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTaa                          <  1:1466603/48‑1 (MQ=255)
                                                    gCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGc  <  1:2060297/71‑1 (MQ=255)
                                                     cTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATc                   <  1:1014925/53‑1 (MQ=255)
                                                      |                                                                    
GGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGC  >  minE/1763502‑1763624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑