Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,892,0191.G100.0% 216.0 / NA 60intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/34);  total (26/34)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*minE1,892,0192.C100.0% 212.2 / NA 60intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/34);  total (26/34)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  minE/1891954‑1892077
                                                                  ||                                                          
gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                           >  1:3149395/1‑66 (MQ=255)
       ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAg                                                   <  1:274714/70‑1 (MQ=255)
        cTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAg                                                   <  1:1194302/69‑1 (MQ=255)
        cTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGaa                                                 >  1:1567973/1‑71 (MQ=255)
         tGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:438099/1‑71 (MQ=255)
           aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               <  1:946418/70‑1 (MQ=255)
           aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               <  1:2584707/70‑1 (MQ=255)
           aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                >  1:1967180/1‑69 (MQ=255)
            aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:2117864/1‑71 (MQ=255)
                attcgattcAGCACCGCTAAAACGACATTTACCGCTCTCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:865453/71‑1 (MQ=255)
                attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:762366/71‑1 (MQ=255)
                attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:2484282/71‑1 (MQ=255)
                 ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         >  1:2134493/1‑70 (MQ=255)
                  tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:1404647/1‑71 (MQ=255)
                  tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:1067384/1‑71 (MQ=255)
                   cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGta                                          >  1:2584298/1‑66 (MQ=255)
                   cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       <  1:2347765/70‑1 (MQ=255)
                   cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       >  1:9827/1‑70 (MQ=255)
                   cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa                                       <  1:15061/70‑1 (MQ=255)
                    gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      <  1:677214/70‑1 (MQ=255)
                    gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      <  1:2523329/70‑1 (MQ=255)
                      ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      >  1:469681/1‑68 (MQ=255)
                      ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      >  1:2907520/1‑68 (MQ=255)
                        cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                                 >  1:499171/1‑71 (MQ=255)
                        cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc                                 >  1:2417043/1‑71 (MQ=255)
                          gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                >  1:2848097/1‑70 (MQ=255)
                          gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                >  1:1284929/1‑70 (MQ=255)
                          gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                >  1:3114437/1‑70 (MQ=255)
                           cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               <  1:2851690/54‑1 (MQ=255)
                           cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc                                               <  1:3194805/54‑1 (MQ=255)
                           cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt                              >  1:1466056/1‑71 (MQ=255)
                             ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcg                            <  1:32177/71‑1 (MQ=255)
                               gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:2295821/1‑52 (MQ=255)
                                cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         <  1:391737/71‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg                                        <  1:2156088/53‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc                      <  1:103112/71‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:214885/70‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:1243313/69‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:2296670/69‑1 (MQ=255)
                                   aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:2448871/69‑1 (MQ=255)
                                     aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                   <  1:2460992/56‑1 (MQ=255)
                                      cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:2803573/40‑1 (MQ=255)
                                        aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg                 <  1:2020763/71‑1 (MQ=255)
                                         cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcg                            >  1:2406274/1‑59 (MQ=255)
                                         cATTTACAGCTCGCTGAACATATCAGCTAAGcaca                                                    >  1:3173038/1‑35 (MQ=16)
                                           tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  <  1:2403462/51‑1 (MQ=255)
                                           tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:3267846/61‑1 (MQ=255)
                                           tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:1901975/61‑1 (MQ=255)
                                             tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc                      >  1:1155029/1‑61 (MQ=255)
                                             tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc                      >  1:2344949/1‑61 (MQ=255)
                                             tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       >  1:969620/1‑60 (MQ=255)
                                             tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt             <  1:1133313/70‑1 (MQ=255)
                                              aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:2778739/1‑45 (MQ=255)
                                              aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc               >  1:819850/1‑67 (MQ=38)
                                               ccGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAt          <  1:2406718/71‑1 (MQ=255)
                                               ccGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAt          <  1:1545812/71‑1 (MQ=255)
                                                cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAt                    <  1:1852718/60‑1 (MQ=255)
                                                cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAt          <  1:1612565/70‑1 (MQ=255)
                                                  ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt       <  1:2415822/71‑1 (MQ=255)
                                                      ctGAACATATCAGCTAAGCACAGAACCGGGGCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTaa   <  1:2830603/71‑1 (MQ=255)
                                                       tGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAc  >  1:3214068/1‑71 (MQ=39)
                                                                  ||                                                          
GCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  minE/1891954‑1892077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: