| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,736,586 | G→T | 7.4% | A55E (GCG→GAG) | rnc ← | RNase III |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,736,586 | 0 | G | T | 7.4% | 228.1 / 3.5 | 67 | A55E (GCG→GAG) | rnc | RNase III |
| Reads supporting (aligned to +/- strand): ref base G (32/30); new base T (3/2); total (35/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCCACGGACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGC > minE/1736516‑1736656 | gCCACGGACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGCGAAACGGTGATAAAgcg > 1:790360/1‑71 (MQ=255) ccACGGACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAgcgc < 1:1033213/71‑1 (MQ=255) aCGGACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCAt > 1:1221253/1‑71 (MQ=255) aCGGACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCAt > 1:19545/1‑71 (MQ=255) aCGGACCAGCGTGGCGCGCATCCGGCTCATATCGCATTCATCCACACGAGGGAAACGGTGATAAAGCGCAt > 1:1544319/1‑71 (MQ=255) ggACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTg < 1:1062133/71‑1 (MQ=255) ggACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTg > 1:208588/1‑71 (MQ=255) ggACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTg < 1:1369670/71‑1 (MQ=255) ggACCAGCGTGGCGCGCATCCGGCTCATATAGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTg > 1:910798/1‑71 (MQ=255) gACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTgg > 1:885264/1‑71 (MQ=255) aCCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGc < 1:68693/71‑1 (MQ=255) aCCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGc < 1:494076/71‑1 (MQ=255) aCCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGc < 1:1639107/71‑1 (MQ=255) tGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg < 1:95491/71‑1 (MQ=255) gcgcATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg > 1:146382/1‑67 (MQ=255) aTCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg < 1:2373027/63‑1 (MQ=255) aTCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg < 1:1238474/63‑1 (MQ=255) aTCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAg < 1:154441/71‑1 (MQ=255) tCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCg < 1:2379161/56‑1 (MQ=255) cGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg > 1:180440/1‑60 (MQ=255) cGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg > 1:271853/1‑60 (MQ=255) cGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGaa < 1:537613/70‑1 (MQ=255) cGGCTCATATCGCCTTCATCCACACGAGGGAAACGGAGATAAAGCGCATTGGCGATaa > 1:321135/1‑58 (MQ=255) cGGCTCATATCGCCTTAATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg > 1:2552428/1‑60 (MQ=255) ggCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAg > 1:310659/1‑62 (MQ=255) ggCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAg > 1:2748833/1‑62 (MQ=255) atatCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCTCATTGGCGATAACGTAGCTCAGaa > 1:3227617/1‑64 (MQ=255) atatCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATagag < 1:775034/69‑1 (MQ=255) tatCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCTCATTGGCGATAACg < 1:2907160/53‑1 (MQ=255) atCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATag < 1:3232428/65‑1 (MQ=255) tctccTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGcc > 1:764369/4‑71 (MQ=255) tCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCTCATTGGCGATAACGTAGCTCAGAATAGAGTCGc > 1:1762110/1‑70 (MQ=255) tCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATa < 1:2421551/63‑1 (MQ=255) tCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATa < 1:2717594/63‑1 (MQ=255) tCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGcc > 1:2303604/1‑71 (MQ=255) cTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTaaa < 1:200772/71‑1 (MQ=255) cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATaa > 1:2097859/1‑42 (MQ=255) cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg > 1:261607/1‑44 (MQ=255) cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACg > 1:88246/1‑44 (MQ=255) cATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTaaa < 1:3032948/68‑1 (MQ=255) aTCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAAt < 1:3145344/70‑1 (MQ=255) tCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATaga < 1:2698181/56‑1 (MQ=255) cacaCGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAAt > 1:2770968/1‑51 (MQ=255) cacaCGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTc > 1:826446/1‑57 (MQ=255) cacGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTc > 1:2609329/1‑67 (MQ=255) cGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATa > 1:347714/1‑48 (MQ=255) cGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATa > 1:883178/1‑48 (MQ=255) cGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACg < 1:493909/71‑1 (MQ=255) gggAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTc < 1:519912/71‑1 (MQ=255) gataCGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGt < 1:3200786/68‑1 (MQ=255) gAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGt < 1:2870161/71‑1 (MQ=255) gAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGt < 1:2403772/71‑1 (MQ=255) gAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGt < 1:182821/71‑1 (MQ=255) aaaCGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATa > 1:2740081/1‑42 (MQ=255) aaCGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTa < 1:1600234/71‑1 (MQ=255) gTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGtt < 1:1009971/71‑1 (MQ=255) gTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGtt < 1:1079629/71‑1 (MQ=255) aTAAAGCTCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGttt < 1:3365777/69‑1 (MQ=255) aTAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTAc > 1:761650/1‑71 (MQ=255) aTAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTAc > 1:2079369/1‑71 (MQ=255) tAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTc < 1:436218/59‑1 (MQ=255) aaaGCTCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTAc > 1:1997124/1‑69 (MQ=255) aaaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTaaa > 1:1430272/1‑53 (MQ=255) aaaGCGAATTGGCGATAACGTAGCTCAGAATAGAGTCg > 1:1131898/1‑38 (MQ=255) aaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTc > 1:780922/1‑36 (MQ=255) aaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCg > 1:1242191/1‑37 (MQ=255) aaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCg > 1:1749556/1‑37 (MQ=255) aaGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCg > 1:2187188/1‑37 (MQ=255) aGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACg < 1:602386/53‑1 (MQ=255) cgcATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTgg < 1:1825211/71‑1 (MQ=255) cgcATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTgg < 1:1536930/71‑1 (MQ=255) gcATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCt < 1:3136866/45‑1 (MQ=255) gcATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGc < 1:3149470/71‑1 (MQ=255) gcATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGc < 1:1292637/71‑1 (MQ=255) gcATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGc < 1:117790/71‑1 (MQ=255) | GCCACGGACCAGCGTGGCGCGCATCCGGCTCATATCGCCTTCATCCACACGAGGGAAACGGTGATAAAGCGCATTGGCGATAACGTAGCTCAGAATAGAGTCGCCTAAAAATTCTAAACGCTCGTTATGTTTACTGCTGGC > minE/1736516‑1736656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |