| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,968,173 | T→G | 8.1% | M153L (ATG→CTG) ‡ | tktA ← | transketolase 1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,968,173 | 0 | T | G | 8.1% | 216.6 / 17.8 | 87 | M153L (ATG→CTG) ‡ | tktA | transketolase 1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base T (33/47); new base G (4/3); total (37/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
CCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTT > minE/1968104‑1968241 | ccAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg < 1:3230136/71‑1 (MQ=255) cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg > 1:247989/1‑70 (MQ=255) cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg > 1:845661/1‑70 (MQ=255) aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg > 1:2606726/1‑69 (MQ=255) aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg > 1:2610651/1‑69 (MQ=255) aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg > 1:2691993/1‑69 (MQ=255) aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGaa < 1:427668/71‑1 (MQ=255) gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAg > 1:2594630/1‑71 (MQ=255) gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAg > 1:3072947/1‑71 (MQ=255) cAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc < 1:3096455/69‑1 (MQ=255) cAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc < 1:1178532/69‑1 (MQ=255) atcGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTa < 1:2030367/69‑1 (MQ=255) aGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc < 1:2654315/68‑1 (MQ=255) aGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTa < 1:1958560/71‑1 (MQ=255) tACCCGCCAGAGAGCAAACTTCGTGGGAGATTCCTTCCATCATGCAGCCGTCGCCCATg < 1:2020731/59‑1 (MQ=255) tACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg < 1:3140370/59‑1 (MQ=255) tACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg < 1:3254777/59‑1 (MQ=255) tACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGaa < 1:3078283/61‑1 (MQ=255) tACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGaa < 1:389248/61‑1 (MQ=255) cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt < 1:1348888/70‑1 (MQ=255) cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt < 1:3218328/70‑1 (MQ=255) cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt < 1:2516158/70‑1 (MQ=255) cccGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt < 1:351745/70‑1 (MQ=255) agagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTc < 1:1766970/71‑1 (MQ=255) gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCg < 1:1318057/71‑1 (MQ=255) gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCg < 1:591497/71‑1 (MQ=255) gagagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCg < 1:1864581/71‑1 (MQ=255) agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAgg > 1:2664212/1‑59 (MQ=255) agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAgg > 1:987709/1‑59 (MQ=255) gagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGAc < 1:2442227/71‑1 (MQ=255) agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAg > 1:47163/1‑56 (MQ=255) agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACa < 1:2500579/71‑1 (MQ=255) agCAAACTTCGTGGGAGATGCCTTCCATCATCCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACa < 1:588497/71‑1 (MQ=255) gCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg < 1:584748/58‑1 (MQ=255) aaCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGaa < 1:2456592/45‑1 (MQ=255) aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTc < 1:105465/71‑1 (MQ=255) cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg < 1:3076438/41‑1 (MQ=255) tCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTg > 1:939055/1‑71 (MQ=255) tCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTg > 1:3228926/1‑71 (MQ=255) agaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg < 1:2713901/44‑1 (MQ=255) agaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCgg > 1:2201190/1‑70 (MQ=255) aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg < 1:2363072/42‑1 (MQ=255) aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg < 1:862404/42‑1 (MQ=255) gCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACa > 1:266404/1‑52 (MQ=255) gCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGt > 1:3164596/1‑71 (MQ=255) ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTa < 1:2864262/35‑1 (MQ=255) ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt < 1:2898709/40‑1 (MQ=255) ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGAc < 1:365292/50‑1 (MQ=255) cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:2998872/71‑1 (MQ=255) cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:1314880/71‑1 (MQ=255) cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:2532176/71‑1 (MQ=255) cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:651863/71‑1 (MQ=255) ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAgg < 1:3110940/35‑1 (MQ=255) ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc > 1:1354808/1‑61 (MQ=255) ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc > 1:192236/1‑61 (MQ=255) ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc > 1:1553926/1‑61 (MQ=255) ttCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc > 1:1870855/1‑61 (MQ=255) ccATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg > 1:1096484/1‑35 (MQ=255) ccATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTgg > 1:3126093/1‑56 (MQ=255) ccATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTgg > 1:2222264/1‑56 (MQ=255) ccATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTgg > 1:1925630/1‑56 (MQ=255) ccATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTaa < 1:2390893/69‑1 (MQ=255) ccATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAAc > 1:837664/1‑71 (MQ=255) tcatGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTc < 1:3151942/40‑1 (MQ=255) tcatGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTc < 1:3155845/40‑1 (MQ=255) tcatGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTc < 1:2912398/40‑1 (MQ=255) tcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAg < 1:1659893/35‑1 (MQ=255) tcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAg < 1:3015124/35‑1 (MQ=255) catGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCg > 1:1623396/1‑49 (MQ=255) atGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTCGa > 1:2901893/1‑40 (MQ=38) atGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTCGa > 1:151091/1‑40 (MQ=38) atGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTCGa > 1:1090580/1‑40 (MQ=38) atGCAGCCGTCGCCGAGGAAGGCGTAGGTGTAGTGGTCGa > 1:3036865/1‑40 (MQ=38) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCg > 1:2106534/1‑48 (MQ=255) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCg > 1:895493/1‑48 (MQ=255) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCg > 1:2457194/1‑48 (MQ=255) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc > 1:1338810/1‑54 (MQ=255) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc > 1:2238623/1‑54 (MQ=255) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTgcgc > 1:1063905/1‑71 (MQ=255) atGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTgcgc > 1:2358366/1‑71 (MQ=255) cGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGc > 1:2413018/1‑71 (MQ=255) gCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtt < 1:657213/70‑1 (MQ=255) cccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttt < 1:61412/71‑1 (MQ=255) cccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttt < 1:1298228/71‑1 (MQ=255) cATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:801916/49‑1 (MQ=255) cATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:2632289/49‑1 (MQ=255) cATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttttt < 1:470905/71‑1 (MQ=255) tGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTa < 1:977623/47‑1 (MQ=255) | CCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTT > minE/1968104‑1968241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |